Isolation and in vitro maintenance of intact colonic crypts from normal human col

描述（由申请人提供）：摘要本文提出的研究的长期目标是开发从组织学正常的人结肠组织和腺瘤性息肉/恶性肿瘤中分离和体外维持完整结肠隐窝的方法。在培养中保持的分离的结肠隐窝将提供独特的培养系统，用于检查潜在的化学预防剂在临床前环境中的作用，以及用于检查结肠中生长调节的各个方面。同样的培养系统，希望，证明是有用的分离和表征干细胞从正常和异常的隐窝。为了达到这个目标，我们将做以下工作：具体目标：从组织学正常的人结肠组织和腺瘤性息肉中分离出隐窝;- 开发培养条件，所述培养条件维持隐窝组织学结构和生物化学功能，并且允许组织学上正常的隐窝以空间和时间上适当的方式通过增殖、分化和凋亡进展，同时允许源自癌前腺瘤性息肉的组织继续生长和扩张。

DESCRIPTION (provided by applicant): Abstract the long-term goal of the research proposed here is to develop methods for the isolation and in vitro maintenance of intact colonic crypts from histologically normal human colon tissue and from adenomatous polyps / malignant tumors. The isolated colonic crypts maintained in culture will provide a unique culture system for examining the effects of potential chemopreventive agents in a preclinical setting as well as for examining various aspects of growth regulation in the colon. The same culture system will, hopefully, prove useful for isolation and characterization of stem cells from normal and aberrant crypts. To achieve the goals of this proposal, we will do the following: Specific Aim: Isolate crypts from histologically normal human colon tissue and from adenomatous polyps; develop culture conditions that maintain crypt histological structure and biochemical function and that allow for progression of the histologically-normal crypts through proliferation, differentiation an apoptosis in a spatially and temporally appropriate manner, while allowing for continued growth and expansion of the tissue derived from premalignant adenomatous polyps.