课题基金 / 基金详情

Global Identification of Mammalian Synaptic Caspase Targets

Global Identification of Mammalian Synaptic Caspase Targets
哺乳动物突触 Caspase 靶标的全局识别
批准号:
8413043
负责人:
James William Mandell
金额:
$18.58万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2012
资助国家:
美国
项目状态:
已结题
起止时间:
2012-04-01 至 2014-05-31

项目摘要

项目成果

James William Mandell的其他基金

相关文献

中文摘要
翻译
细胞凋亡,或称程序性细胞死亡,是所有多细胞生物的一个特征
英文摘要
Apoptosis, or programmed cell death, is a feature of all multicellular organisms and has critical roles in development, homeostasis, and disease. This intensely studied process is characterized by and requires the activation of a family of proteolytic enzymes known as caspases (cysteine-dependent aspartate-directed proteases). Caspases cleave a large number of specific target proteins, orchestrating the orderly transformation of intact cells into fragments that may be removed from the body by phagocytic cells. Recent evidence points to non-apoptotic roles for caspases in the brain, including the regulation of synaptic plasticity. Caspase activation was recently shown to precede tangle formation in a mouse model of Alzheimer's disease. However, surprisingly little is known about the global scope of neuronal synaptic proteins targeted by caspases, the specific sites of cleavage, and mechanisms by which caspase- mediated cleavages alter neuronal function. This proposal will N-terminus labeling approach to positively select cleaved proteins from the complex protein content of synaptosomes or brain lysates, allowing systematic identification caspase targets in synaptosomal preparations as well as in discrete brain regions and models of neuronal apoptotic induction. The analysis will not only identify the protein substrates of caspases in synapses, but will also reveal the precise site of proteolytic cleavage, providing immediate molecular insight into the function of the cleavage event. The information derived from this project will not only stimulate further research on the functional roles of caspase cleavage of synaptic targets, but will also provide immediate candidate biomarkers for human neurological disorders, including trauma, stroke, and neurodegenerative diseases.
期刊论文(1)
专著(0)
科研奖励(0)
会议论文
Circulating Non-coding RNAs as Biomarkers of Inclusion Body Myositis
  • 批准号:
    8893583
  • 项目类别:
  • 资助金额:
    $20.86万
  • 财政年份:
    2015
  • 负责人:
    James William Mandell
  • 依托单位:
Global Identification of Mammalian Synaptic Caspase Targets
  • 批准号:
    8302120
  • 项目类别:
  • 资助金额:
    $23.1万
  • 财政年份:
    2012
  • 负责人:
    James William Mandell
  • 依托单位:
Astroglial Phagocytosis of Degenerating Neurons and Axons: Role of Rac1 and ELMO1
  • 批准号:
    7835752
  • 项目类别:
  • 资助金额:
    $18.75万
  • 财政年份:
    2009
  • 负责人:
    James William Mandell
  • 依托单位:
Functions of ERK and p38 MAP Kinases in Astrogliosis
  • 批准号:
    6984087
  • 项目类别:
  • 资助金额:
    $31.94万
  • 财政年份:
    2003
  • 负责人:
    James William Mandell
  • 依托单位: