Nanotechnology-Based Detection of Mycoplasma pneumoniae

基于纳米技术的肺炎支原体检测

• 批准号: 8522149
• 负责人: DUNCAN C KRAUSE
• 金额: $ 19.07万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-15 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8522149
• 关键词: Accounting; Advanced Development; Asthma; Biosensing Techniques; Biosensor; Blinded; Bronchitis; Child; Chlamydophila pneumoniae; Chronic; Chronic Obstructive Airway Disease; Chronic lung disease; Clinical; Communities; Complex; Data; Deposition; Detection; Development; Diagnosis; Diagnostic tests; Disease; Electromagnetic Fields; Electromagnetics; Emerging Technologies; Exhibits; False Negative Reactions; Goals; Human; Infection; Infectious Agent; Lead; Lung; Methods; Moraxella (Branhamella) catarrhalis; Morbidity - disease rate; Mycoplasma; Mycoplasma pneumoniae; Nanotechnology; Pattern Recognition; Pharyngeal structure; Physicians; Pneumonia; Publishing; Raman Spectrum Analysis; Respiratory System; Respiratory tract structure; Sampling; Sensitivity and Specificity; Serologic tests; Serological; Serum; Silver; Simulate; Specimen; Streptococcus pyogenes; Surface; Swab; Testing; Virus; Visit; Walking; base; clinical application; clinically relevant; cohort; improved; inhibitor/antagonist; nanofabrication; nanorod; pathogen; rapid diagnosis; secondary infection; transmission process; vapor; young adult

DESCRIPTION (provided by applicant): Mycoplasma pneumoniae is a significant human respiratory tract pathogen, causing bronchitis and atypical or "walking" pneumonia. M. pneumoniae accounts for 20% of all community-acquired pneumonia and is the leading cause of pneumonia in older children and young adults. Serologic testing is the primary method for diagnosis due to the significant challenges posed by direct culture but suffers from severe limitations, including the need for paired sera obtained at separate physician visits, and is thus impractical for rapid diagnosis. PCR can exhibit high sensitivity and yield positive detection sooner but is prone to false-negatives from reaction inhibitors. The inability to provide rapid and definitive diagnosis delays initiation of appropriate treatment, prolongs morbidity, and increases the likelihood of continued transmission, secondary infections, and long-term sequelae, including chronic lung disease associated with COPD and asthma. Lack of a simple, reliable, rapid diagnostic test is thus a critical barrier to improved control of M. pneumoniae disease. Application of nanotechnology to biosensor development is yielding direct, rapid, and sensitive pattern-recognition approaches for detection of infectious agents. We have shown that nanofabrication by glancing angle vapor deposition produces Ag nanorod arrays (NA) exhibiting extremely high electromagnetic field enhancements for surface-enhanced Raman spectroscopy (SERS). Paired with chemometric analysis this platform can rapidly detect and distinguish with outstanding sensitivity and specificity the Raman spectra of viruses and mycoplasmas, and shows great promise in its potential to improve diagnosis of M. pneumoniae infections. Our overall goal is to advance the application of NA-SERS to rapid and sensitive mycoplasma detection in clinical specimens, building upon critical published data that demonstrate its capacity to detect M. pneumoniae at clinically relevant sensitivity in simulated and true clinical throat swab samples. Our application has two specific aims, shifting the project from proof-of-concept toward clinical application: (1) expand analysis of spiked and control samples using throat swabs from a broader cohort, including direct comparison to qPCR; analyze spectral signatures of commensal mycoplasmas and assess their impact on M. pneumoniae detection; and assess NA- SERS discriminatory ability in mixed infections with other bacterial pathogens including Chlamydophila pneumoniae; and (2) test by NA-SERS, true clinical throat swab samples previously established as positive or negative for M. pneumoniae by PCR and culture, in unblinded and blinded analyses; and characterize M. pneumoniae isolates obtained globally to determine whether strain diversity impacts detection by NA-SERS in spiked throat swab samples.

描述（由申请人提供）：肺炎支原体是一种重要的人类呼吸道病原体，可引起支气管炎和非典型或“行走”肺炎。肺炎支原体占所有社区获得性肺炎的20%，是大龄儿童和年轻人肺炎的主要原因。由于直接培养带来的重大挑战，血清学检测是诊断的主要方法，但存在严重的局限性，包括需要在单独的医生就诊时获得成对的血清，因此对于快速诊断是不切实际的。PCR可以表现出高灵敏度和产生阳性检测更快，但容易假阴性的反应抑制剂。无法提供快速和有效的服务

项目成果

Highly Sensitive Detection and Differentiation of Endotoxins Derived from Bacterial Pathogens by Surface-Enhanced Raman Scattering.

• DOI: 10.3390/bios11070234
• 发表时间: 2021-07-11
• 期刊: Biosensors
• 作者: Wu X;Zhao Y;Zughaier SM
• 通讯作者: Zughaier SM

Layer-by-layer polyelectrolyte encapsulation of Mycoplasma pneumoniae for enhanced Raman detection.

肺炎支原体的层层聚电解质封装用于增强拉曼检测。

• DOI: 10.1039/c4an00596a
• 发表时间: 2014
• 期刊: The Analyst
• 作者: Rivera-Betancourt,OmarE;Sheppard,EdwardS;Krause,DuncanC;Dluhy,RichardA
• 通讯作者: Dluhy,RichardA

Specificity and Strain-Typing Capabilities of Nanorod Array-Surface Enhanced Raman Spectroscopy for Mycoplasma pneumoniae Detection.

纳米棒阵列表面增强拉曼光谱检测肺炎支原体的特异性和菌株分型能力。

• DOI: 10.1371/journal.pone.0131831
• 发表时间: 2015
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Henderson,KelleyC;Benitez,AlvaroJ;Ratliff,AmyE;Crabb,DonnaM;Sheppard,EdwardS;Winchell,JonasM;Dluhy,RichardA;Waites,KenB;Atkinson,TPrescott;Krause,DuncanC
• 通讯作者: Krause,DuncanC

Culture-free diagnostics of Pseudomonas aeruginosa infection by silver nanorod array based SERS from clinical sputum samples.

• DOI: 10.1016/j.nano.2014.04.010
• 发表时间: 2014-11
• 期刊: NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
• 影响因子: 5.4
• 作者: Wu, Xiaomeng;Chen, Jing;Li, Xibo;Zhao, Yiping;Zughaier, Susu M.
• 通讯作者: Zughaier, Susu M.

The multivariate detection limit for Mycoplasma pneumoniae as determined by nanorod array-surface enhanced Raman spectroscopy and comparison with limit of detection by qPCR.

• DOI: 10.1039/c4an01141d
• 发表时间: 2014-12-21
• 期刊: The Analyst
• 作者: Henderson KC;Sheppard ES;Rivera-Betancourt OE;Choi JY;Dluhy RA;Thurman KA;Winchell JM;Krause DC
• 通讯作者: Krause DC