National Biomedical EPR Center

描述(由申请人提供)：美国国立卫生研究院资助的赠款的主要用途是使用氮氧化物自由基自旋标记来探测分子结构的位点定向自旋标记(SDSL)领域。本申请的技术研究与开发(TR&D)部分继续支持国家生物医学EPR中心，P41研究资源，重点是EPR的SDSL应用的技术增强。研发部分与协作部分紧密相连，大多数合作都属于SDSL类别。涉及金属离子的应用是NLH支持的下一个使用EPR的赠款，在本提交中，服务部分特别强调向该社区提供EPR能力。培训和传播部分具有广泛的基础和活力。我们介绍了 非绝热快速扫描(NARS)光谱学技术，作为EPR光谱学中相敏检测(PSD)的通用替代品。NAR能力将在L、X波段、Q波段、W波段和D波段建立。在本资助期间，在W波段论证了绝热快速扫描(ARS)的扩展，建立了将ARS技术与任意波形发生器(AWG)扫描相结合用于傅立叶变换EPR的可行性。磁场扫描的中间“通道”范围将被用来探测非常慢的运动。将在Q、W和D频段开发微波扫频。研发项目1专注于L、-X波段和Q波段的新技术开发；项目3将该技术推广到W和D波段；项目2专注于L波段的两个应用：室温至40A的距离测定和L波段电子-电子双共振(ELDOR)技术用于蛋白质和膜静电势的测量。~(14)N自旋标记的M=0谱线很窄，而且非常接近各向同性，这使它成为这两个实验的理想探针。提出了为NARS探测量身定做的新型谐振器。这十个合作与三个研发项目联系得非常紧密。值得注意的是，加州大学洛杉矶分校韦恩·L·哈贝尔教授对该项目的强烈支持，他也是该中心外部咨询委员会的主席。合作显示了SDSL社区的广泛兴趣。本意见书中的研发主题是用于SDSL研究的广泛微波频率的非绝热、绝热和快速通道技术开发。NIH记者数据库显示了124项使用EPR的R0I研究拨款。我们为这些拨款的持有者提供服务，以加强国家的生物医学研究。在本供资期间取得的进展包括220篇论文。

DESCRIPTION (provided by applicant): The dominant usage in NIH-funded grants is in the field of site-directed spin-labeling (SDSL) using nitroxide radical spin labels to probe molecular structure. The Technology Research and Development (TR&D) component of this application for continued support of the National Biomedical EPR Center, a P41 Research Resource, focuses on technology enhancement for SDSL applications of EPR. The TR&D component is tightly linked to the Collaborative component, with most collaborations falling in the SDSL classification. Applications involving metal ions are next among NlH-supported grants using EPR, and in this submission, special emphasis is placed in the Service component on making EPR capabilities available to this community. Training and Dissemination components are broadly based and vigorous. We introduced the technology of non-adiabatic rapid sweep (NARS) spectroscopy in the current funding period as a general purpose replacement for phase-sensitive detection (PSD) in EPR spectroscopy. NARS capabilities will be built at L-, X-, Q-, W-, and D-band. Extension to adiabatic rapid sweep (ARS) was demonstrated at W-band during the current funding period, establishing feasibility for Fourier transform EPR using ARS technology with arbitrary waveform generator (AWG) sweep, which is proposed. The intermediate "passage" range of magnetic field sweep will be used to probe very slow motions. Microwave frequency sweep will be developed at Q-, W-, and D-band. TR&D Project 1 focuses on development of the new technology at L,- X-, and Q-band; Project 3 carries the technology to W- and D-band; and Project 2 focuses on two L-band applications: distance determination at room temperature to 40 A and L-band electron-electron double resonance (ELDOR) for measurement of protein and membrane electrostatic potentials. The M = 0 line of perdeutero 14N spin labels is narrow and very nearly isotropic, making it an ideal probe for these two experiments. New resonators tailored for NARS detection are proposed. The ten collaborations are very tightly linked to the three TR&D projects. The strong support letter for this project from Prof. Wayne L. Hubbell, UCLA, who is also chairman of the External Advisory Committee for the Center, is notable. Collaborations demonstrate widespread interest in the SDSL community. The TR&D theme in this submission is non-adiabatic, adiabatic, and rapid passage technology development across a wide range of microwave frequencies for SDSL research. The NIH RePORTER database reveals 124 R0I research grants that use EPR. We serve the holders of these grants to enhance the nation's biomedical research. Progress in the current funding period includes 220 papers.