Fluorescence Microscopy

荧光显微镜

• 批准号: 9143529
• 负责人: JAMES W PUTNEY
• 金额: $ 119.02万
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9143529
• 关键词: ATP binding cassette transporter 1; American; Americas; Astrocytes; Base Excision Repairs; Bibliography; Biochemical Pharmacology; Biochemistry; Biology; Breast Cancer Cell; Calcium; Calcium Channel; Cell Survival; Cellular biology; Chemotactic Factors; Chemotaxis; Collaborations; Communication; Complement Receptor; Complex; DNA Polymerase beta; DNA Repair; Dendritic Cells; Development; Dipeptidyl Peptidases; Dipeptidyl-Peptidase IV; Disease; Dissociation; Dominant-Negative Mutation; Ensure; Epigenetic Process; Equipment; Fibroblasts; Fluorescence; Fluorescence Microscopy; Gene Expression; Glutamates; Goals; Hematopoietic; Hippocampus (Brain); Human; Hydrogen Peroxide; Image; Infiltration; Journals; Knowledge; Lactation; Lysine; Maneb; Mediating; Mediator of activation protein; Mission; Mitochondria; Modeling; Molecular; Molecular Genetics; Molecular Neurobiology; Molecular and Cellular Biology; Monitor; Mus; NADPH Oxidase; National Institute of Environmental Health Sciences; Nature; Neuroglia; Nicotinic Receptors; Nuclear Translocation; Oocytes; Optics; Ovarian; Paraquat; Parkinson Disease; Phosphorylation; Polycomb; Proteins; Proteomics; Protocols documentation; Psoriasis; Publications; Receptor Activation; Receptor Signaling; Regulation; Research; Research Personnel; Role; STIM1 gene; Science; Scientist; Services; Signal Transduction; Single Strand Break Repair; Skin; Societies; Spliced Genes; Substance P; Substance P Receptor; Substantia nigra structure; Techniques; Technology; The Sun; Toll-like receptors; Training; Translation Initiation; United States National Academy of Sciences; Variant; Work; XRCC1 gene; alpha synuclein; base; density; egg; fluorescence imaging; granulosa cell; histone modification; human CYP2C9 protein; human RGS2 protein; investigator training; irradiation; macrophage; meetings; migration; neutrophil; oxidative DNA damage; premature; programs; promoter; respiratory; skills; theca cell; tool; transmission process

In meeting the goals and objectives of FMIC, the center continues to: -Service and maintain state-of-the-art equipment for unlimited access by intramural scientists. -Provide investigator training in the use and analysis of fluorescence and optical-based techniques. -Develop and optimize experimental protocols in collaboration with intramural scientists. -Maintain and develop state-of-the-art analysis tools and protocols in collaboration with investigators -Monitor and incorporate emerging fluorescence and optical technologies that will support the mission of NIEHS into FMIC. During this fiscal year, FMIC has collaborated and supported 50 Principle Investigators from all 5 programs within the NIEHS DIR. Some publications from this FY resulting from work in the center is listed in the bibliography below: Bibliography Bernhardt, M.L., Lowther, K.M., Padilla-Banks, E., McDonough, C.E., Lee, K.N., Evsikov, A.V., Uliasz, T.F., Chidiac, P., Williams, C.J., and Mehlmann, L.M. (2015). Regulator of G-protein signaling 2 (RGS2) suppresses premature calcium release in mouse eggs. Development 142, 2633-2640. Chen, S.H., Oyarzabal, E.A., Sung, Y.F., Chu, C.H., Wang, Q., Chen, S.L., Lu, R.B., and Hong, J.S. (2015). Microglial regulation of immunological and neuroprotective functions of astroglia. Glia 63, 118-131. Cheng, Q., and Yakel, J.L. (2015). The effect of alpha7 nicotinic receptor activation on glutamatergic transmission in the hippocampus. Biochemical pharmacology. Chowdhury, S.M., Zhu, X., Aloor, J.J., Azzam, K.M., Gabor, K.A., Ge, W., Addo, K.A., Tomer, K.B., Parks, J.S., and Fessler, M.B. (2015). Proteomic Analysis of ABCA1-Null Macrophages Reveals a Role for Stomatin-Like Protein-2 in Raft Composition and Toll-Like Receptor Signaling. Molecular & cellular proteomics : MCP 14, 1859-1870. Davis, F.M., Janoshazi, A., Janardhan, K.S., Steinckwich, N., D'Agostin, D.M., Petranka, J.G., Desai, P.N., Roberts-Thomson, S.J., Bird, G.S., Tucker, D.K., et al. (2015). Essential role of Orai1 store-operated calcium channels in lactation. Proceedings of the National Academy of Sciences of the United States of America 112, 5827-5832. Desai, P.N., Zhang, X., Wu, S., Janoshazi, A., Bolimuntha, S., Putney, J.W., and Trebak, M. (2015). Multiple types of calcium channels arising from alternative translation initiation of the Orai1 message. Science signaling 8, ra74. Englert, N.A., Luo, G., Goldstein, J.A., and Surapureddi, S. (2015). Epigenetic modification of histone 3 lysine 27: mediator subunit MED25 is required for the dissociation of polycomb repressive complex 2 from the promoter of cytochrome P450 2C9. The Journal of biological chemistry 290, 2264-2278. Gassman, N.R., Coskun, E., Stefanick, D.F., Horton, J.K., Jaruga, P., Dizdaroglu, M., and Wilson, S.H. (2015). Bisphenol a promotes cell survival following oxidative DNA damage in mouse fibroblasts. PloS one 10, e0118819. Gassman, N.R., and Wilson, S.H. (2015). Micro-irradiation tools to visualize base excision repair and single-strand break repair. DNA repair 31, 52-63. Horton, J.K., Gassman, N.R., Dunigan, B.D., Stefanick, D.F., and Wilson, S.H. (2015). DNA polymerase beta-dependent cell survival independent of XRCC1 expression. DNA repair 26, 23-29. Kumar, A., Leinisch, F., Kadiiska, M.B., Corbett, J., and Mason, R.P. (2015). Formation and Implications of Alpha-Synuclein Radical in Maneb- and Paraquat-Induced Models of Parkinson's Disease. Molecular neurobiology. Liu, C., Peng, J., Matzuk, M.M., and Yao, H.H. (2015). Lineage specification of ovarian theca cells requires multicellular interactions via oocyte and granulosa cells. Nature communications 6, 6934. Nakano, H., Moran, T.P., Nakano, K., Gerrish, K.E., Bortner, C.D., and Cook, D.N. (2015). Complement receptor C5aR1/CD88 and dipeptidyl peptidase-4/CD26 define distinct hematopoietic lineages of dendritic cells. J Immunol 194, 3808-3819. Steinckwich, N., Myers, P., Janardhan, K.S., Flagler, N.D., King, D., Petranka, J.G., and Putney, J.W. (2015). Role of the store-operated calcium entry protein, STIM1, in neutrophil chemotaxis and infiltration into a murine model of psoriasis-inflamed skin. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29, 3003-3013. Wang, Q., Oyarzabal, E., Wilson, B., Qian, L., and Hong, J.S. (2015a). Substance P enhances microglial density in the substantia nigra through neurokinin-1 receptor/NADPH oxidase-mediated chemotaxis in mice. Clin Sci (Lond) 129, 757-767. Wang, S., Chu, C.H., Stewart, T., Ginghina, C., Wang, Y., Nie, H., Guo, M., Wilson, B., Hong, J.S., and Zhang, J. (2015b). alpha-Synuclein, a chemoattractant, directs microglial migration via H2O2-dependent Lyn phosphorylation. Proceedings of the National Academy of Sciences of the United States of America 112, E1926-1935. Yang, J., Bennett, B.D., Luo, S., Inoue, K., Grimm, S.A., Schroth, G.P., Bushel, P.R., Kinyamu, H.K., and Archer, T.K. (2015). LIN28A Modulates Splicing and Gene Expression Programs in Breast Cancer Cells. Molecular and cellular biology 35, 3225-3243. Yong, S.T., Nguyen, H.N., Choi, J.H., Bortner, C.D., Williams, J., Pulloor, N.K., Krishnan, M.N., and Shears, S.B. (2015). Identification of a functional nuclear translocation sequence in hPPIP5K2. BMC cell biology 16, 17. Young, M.J., Humble, M.M., DeBalsi, K.L., Sun, K.Y., and Copeland, W.C. (2015). POLG2 disease variants: analyses reveal a dominant negative heterodimer, altered mitochondrial localization and impaired respiratory capacity. Human molecular genetics 24, 5184-5197.

在达到FMIC的目标和目标时，该中心继续： - 服务和维护最先进的设备，以无限制地访问壁内科学家。 - 提供和分析荧光和基于光学技术的研究者培训。 - 与壁内科学家合作开发和优化实验方案。 - 与调查人员合作培养和开发最先进的分析工具和协议 -Monitor并结合了新兴的荧光和光学技术，这些技术将支持NIEHS的任务为FMIC。 在这个财政年度，FMIC合作并支持Niehs Dir中所有5个计划的50名主要调查人员。 以下书目中列出了该中心工作中该FY的一些出版物： 参考书目 Bernhardt，M.L.，Lowther，K.M.，Padilla-Banks，E. G蛋白信号2（RGS2）的调节剂抑制了小鼠卵中的过早钙释放。开发142，2633-2640。 Chen，S.H.，Oyarzabal，E.A.，Sung，Y.F.，Chu，C.H.，Wang，Q.，Chen，S.L.，Lu，R.B。和Hong，J.S。 （2015）。星形胶质细胞的免疫学和神经保护功能的小胶质调节。 Glia 63，118-131。 Cheng，Q。和Yakel，J.L。（2015）。 α7烟碱受体激活对海马中谷氨酸能传播的影响。生化药理学。 Chowdhury，S.M.，Zhu，X.，Aloor，J.J.，Azzam，K.M.，Gabor，K.A.，GE，W.，Addo，K.A.，Tomer，K.B. （2015）。 ABCA1-NULL巨噬细胞的蛋白质组学分析揭示了类似烟蛋白样蛋白-2在筏组成和Toll样受体信号传导中的作用。分子和细胞蛋白质组学：MCP 14，1859-1870。 Davis，F.M.，Janoshazi，A. （2015）。 Orai1商店经营的钙通道在泌乳中的重要作用。美利坚合众国国家科学院论文集112，5827-5832。 Desai，P.N.，Zhang，X.，Wu，S.，Janoshazi，A.，Bolimuntha，S.，Putney，J.W。和Trebak，M。（2015年）。由ORAI1消息的替代翻译启动引起的多种类型的钙通道。科学信号8，RA74。 N.A. Englert，Luo，G.，Goldstein，J.A。和Surapureddi，S。（2015年）。组蛋白3赖氨酸27的表观遗传学修饰：介体亚基Med25是从细胞色素P450 2C9启动子中解离Polycomb抑制复合物2所必需的。生物化学杂志290，2264-2278。 加斯曼（Gassman） （2015）。在小鼠成纤维细胞中氧化性DNA损伤后，双足A促进细胞存活。 PLOS ONE 10，E0118819。 Gassman，N.R。和S.H. Wilson （2015）。可视化基础切除修复和单链断裂修复的微辐照工具。 DNA维修31，52-63。 霍顿（J.K. （2015）。 DNA聚合酶β依赖性细胞存活与XRCC1表达无关。 DNA维修26，23-29。 库马尔（A.在Maneb和Paraquat诱导的帕金森氏病模型中，α-核蛋白自由基的形成和含义。分子神经生物学。 Liu，C.，Peng，J.，Matzuk，M.M。和Yao，H.H。（2015）。卵巢theca细胞的谱系规范需要通过卵母细胞和颗粒细胞进行多细胞相互作用。自然通讯6，6934。 Nakano，H.，Moran，T.P.，Nakano，K.，Gerrish，K.E.，Bortner，C.D。和Cook，D.N。（2015）。补体受体C5AR1/CD88和二肽基肽酶-4/CD26定义了树突状细胞的不同造血谱系。 J Immunol 194，3808-3819。 Steinckwich，N.，Myers，P.，Janardhan，K.S。，N.D. Flagler，King，D.，Petranka，J.G。和J.W. Putney （2015）。储存量的钙进入蛋白STIM1在中性粒细胞趋化性和浸润中的作用，成为牛皮癣感染皮肤的鼠模型。 FASEB杂志：美国实验生物学联合会的官方出版物29，3003-3013。 Wang，Q.，Oyarzabal，E.，Wilson，B.，Qian，L。和Hong，J.S。 （2015a）。 PESTANCE P通过Neurokinin-1受体/NADPH氧化酶介导的小鼠中的Nigra中的小胶质细胞密度增强。 Clin Sci（Lond）129，757-767。 Wang，S.，Chu，C.H.，Stewart，T.，Ginghina，C.，Wang，Y.，Nie，H.，Guo，M.，Wilson，B.，Hong，J.S。和Zhang，J。（2015b）。 α-核蛋白是一种趋化剂，通过H2O2依赖性Lyn磷酸化指导小胶质细胞迁移。美国美国国家科学院论文集112，E1926-1935。 Yang，J.，Bennett，B.D.，Luo，S.，Inoue，K.，Grimm，S.A.，Schroth，G.P.，Bushel，P.R。，Kinyamu，H.K。和Archer，T.K。 （2015）。 LIN28A调节乳腺癌细胞中的剪接和基因表达程序。分子和细胞生物学35，3225-3243。 Yong，S.T.，Nguyen，H.N.，Choi，J.H.，Bortner，C.D.，Williams，J.，Pulloor，N.K.，Krishnan，M.N。和Shears和S.B. （2015）。 HPPIP5K2中功能性核转运序列的鉴定。 BMC细胞生物学16，17。 Young，M.J.，Humble，M.M.，Debalsi，K.L.，Sun，K.Y。和W.C. Copeland （2015）。 POLG2疾病变异：分析显示出主要的负异二聚体，线粒体定位改变和呼吸能力受损。人分子遗传学24，5184-5197。