Nitrogenase assembly mechanism

固氮酶组装机制

• 批准号: 8598093
• 负责人: Markus W Ribbe
• 金额: $ 30.87万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2016-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8598093
• 关键词: Binding; Binding Sites; Biochemical; Biomimetics; Carbon; Cluster Analysis; Complex; Core Assembly; Enzymes; Event; Genetic; Glutamine; Health; Homologous Gene; Human; Iron; Knowledge; Label; Location; Maps; Metals; Molybdenum; Nitrogen; Nitrogenase; Pathway interactions; Population; Reaction; Research; Scheme; Site; Source; Structure; Testing; Variant; biological systems; catalyst; chemical synthesis; comparative; homocitrate; insight; interstitial; metal complex; protein protein interaction; public health relevance; tetrathiomolybdate

DESCRIPTION (provided by applicant): Nitrogenase reaction represents a major source of the usable form of nitrogen that supports the existence of human population. As such, understanding how small building blocks are assembled into a functional nitrogenase entity is of significant relevance to human health. Using combined genetic, biochemical, spectroscopic and structural approaches, we propose to investigate how M-cluster, the active center of molybdenum nitrogenase, is assembled. Specifically, we will examine the formation of a Fe-S core of M-cluster, the maturation of Fe-S core into an M-cluster, and the transfer of M-cluster from the assembly site to its target location in great detail. Through our proposed studies, we expect to refine the biosynthetic pathway of M-cluster, which will provide crucial insights into th structural-functional relationship of nitrogenase and the general assembly mechanism of complex metal clusters in biological systems.

描述（由申请人提供）：氮酶反应代表了支持人口存在的氮的可用形式的主要来源。因此，了解如何将小的构建块组装到功能性氮酶实体中与人类健康具有重要意义。使用遗传，生化，光谱和结构方法的组合，我们建议研究如何组装钼氮基酶的活性中心M-Cluster。具体而言，我们将研究M-Cluster的Fe-S核心的形成，Fe-S Core的成熟到M-Cluster，以及将M-Cluster从组装站点转移到其目标位置的转移。通过我们提出的研究，我们希望完善M-Cluster的生物合成途径，这将为生物系统中氮气和复杂金属簇的大会机制提供至关重要的见解。