Nitrogenase Assembly Mechanism

固氮酶组装机制

• 批准号: 8197578
• 负责人: Markus W Ribbe
• 金额: $ 28.94万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2012-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8197578
• 关键词: Address; Ammonia; Azotobacter vinelandii; Binding Sites; Bioavailable; Biochemical; Biogenesis; Biological; Biological Models; Chemicals; Complex; Development; Funding; Future; Genetic; Goals; Health; Human; In Situ; Investigation; Metalloproteins; Metals; Molecular; Molybdenum; Molybdoferredoxin; Nitrogen; Nitrogenase; Nucleic Acids; Nucleotides; Outcome Study; Pathway interactions; Population; Process; Proteins; Reaction; Research; Role; Site; biological systems; chemical synthesis; cost; economic value; interest; metalloenzyme

PROJECT SUMMARY/ABSTRACT Nitrogenase is a complex metalloenzyme that catalyzes one of the most remarkable chemical transformations in biological systems: the nucleotide-dependent reduction of atmospheric dinitrogen to bioavailable ammonia. There is an outstanding interest in decoding the assembly mechanism of nitrogenase, not only because of its significant relevance to nitrogenase-specific reactions, but also because of its tremendous implication for general metalloprotein biogenesis. Here we propose to greatly expand our understanding of the nitrogenase assembly process by combined genetic, biochemical, spectroscopic and structural approaches. The MoFe protein of the molybdenum nitrogenase of Azotobacter vinelandii will be the focus of the proposed investigation. Through our proposed studies, we expect to further refine the biosynthetic pathways of MoFe protein and its associated clusters, a continuous effort with an ultimate goal to unravel the molecular mechanism of nitrogenase assembly. Furthermore, using nitrogenase as a model system, we also hope to provide general themes of biological metal cluster assembly, which may, in the long run, prove instrumental in developing successful strategies for the chemical synthesis of complex metalloclusters. .

项目总结/文摘