Evaluating NLR Modulation of Canonical and Non-Canonical NF-kB Signaling in IBD

评估 IBD 中规范和非规范 NF-kB 信号传导的 NLR 调制

基本信息

  • 批准号:
    8943147
  • 负责人:
  • 金额:
    $ 8.05万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2015
  • 资助国家:
    美国
  • 起止时间:
    2015-06-01 至 2017-05-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Crohn's disease (CD) and ulcerative colitis (UC) are common manifestations of inflammatory bowel disease (IBD). These two debilitating disorders afflict approximately 1.4 million Americans and over 4 million people worldwide. Previous work from our lab has identified a novel sub-group of cellular proteins from the NLR family of pattern recognition receptors that function to negatively regulate inflammation and tumor development during IBD. We have previously shown that the NLR NLRP12 is a potent modulator of experimental colitis and inflammation driven tumorigenesis in pre-clinical mouse models. During IBD, NLRP12 modulates gastrointestinal inflammation through the attenuation of either canonical or non-canoincal NF-κB signaling. While the role of the canonical NF-κB signaling pathway during IBD is well established, the contribution of the non-canonical NF-κB cascade is relatively uncharacterized. Our previous findings indicate that a pair of chemokines associated with non-canonical NF-κB signaling is significantly up-regulated in the absence of NLRP12 and associated with increased IBD pathobiology in mice. These same chemokines are also dysregulated in human IBD patients. For example, our preliminary data revealed that the NLRP12 regulated chemokine CXCL13 is up-regulated 42- and 23-fold in CD and UC patients that are unresponsive to infliximab, respectively. Together, these data illustrate the importance of NLRP12 and non-canonical NF-κB signaling during IBD; however, the underlying mechanism/s is still unresolved. One hypothesis suggests that NLRP12 attenuates canonical NF-κB signaling during IBD; whereas, a second hypothesis that is supported by our preliminary data, indicates that NLRP12 inhibits the non-canoncial NF-κB signaling cascade. The apparent discrepancies in the hypothesized mechanisms can be reconciled by considering that the canonical NF-κB pathway can be significantly influenced by noncanoncial NF-κB signaling. Likewise, it is possible that these pathways are differentially modulated through cell type and/or temporal specific mechanisms that can significantly impact IBD pathogenesis. Here, I propose an extension to research project (K01-DK092355) to better evaluate these two prevailing hypotheses. The overall goal of this proposal is to generate a panel of mice that are deficient in canonical and non-canonical NF-κB signaling following cell specific Cre-recombinase disruption to better characterize the mechanism associated with NLRP12 modulation of IBD. NLRs with negative regulatory functions, such as NLRP12, and non-canonical NF-κB signaling during IBD represent areas that are currently understudied and not well defined. The mouse lines and data generated by the proposed studies will serve as preliminary data for a future R01 submission. The evaluation of these unique pathways in the context of IBD represent a novel direction that will contribute to future discoveries associated with immune system homeostasis in the gut and lead to new therapeutic strategies targeting these diseases.
描述(由申请人提供):克罗恩病(CD)和溃疡性结肠炎(UC)是炎症性肠病(IBD)的常见表现。这两种使人衰弱的疾病折磨着大约140万美国人和全世界超过400万人。我们实验室之前的工作已经从NLR模式识别受体家族中发现了一个新的细胞蛋白亚群,该亚群在IBD期间对炎症和肿瘤的发展起负性调节作用。我们之前已经证明NLR NLRP12在临床前小鼠模型中是实验性结肠炎和炎症驱动的肿瘤发生的有效调节剂。在IBD期间,NLRP12通过减弱典型或非典型NF-κB信号来调节胃肠道炎症。虽然典型NF-κB信号通路在IBD中的作用已经确立,但非典型NF-κB级联的作用相对不明确。我们之前的研究结果表明,在NLRP12缺失的情况下,一对与非规范NF-κB信号相关的趋化因子显著上调,并与小鼠IBD病理生物学增加有关。这些趋化因子在人类IBD患者中也出现了失调。例如,我们的初步数据显示,NLRP12调节的趋化因子CXCL13在对英夫利昔单抗无反应的CD和UC患者中分别上调42倍和23倍。总之,这些数据说明了NLRP12和非典型NF-κB信号在IBD中的重要性;然而,潜在的机制仍未得到解决。一种假说认为NLRP12在IBD期间减弱了典型的NF-κB信号传导;然而,我们的初步数据支持的第二个假设表明,NLRP12抑制非标准NF-κB信号级联。考虑到典型的NF-κB通路可以受到非典型NF-κB信号的显著影响,这些假设机制的明显差异可以得到调和。同样,这些途径可能通过细胞类型和/或时间特异性机制进行差异调节,从而显著影响IBD的发病机制。在这里,我建议对研究项目(K01-DK092355)进行扩展,以更好地评估这两种流行的假设。本研究的总体目标是建立一组在细胞特异性cre -重组酶破坏后缺乏规范和非规范NF-κB信号的小鼠,以更好地表征NLRP12调节IBD的相关机制。具有负调控功能的nlr,如NLRP12和IBD期间的非规范NF-κB信号传导,代表了目前研究不足且未明确定义的领域。拟议研究产生的小鼠系和数据将作为未来R01提交的初步数据。在IBD背景下对这些独特途径的评估代表了一个新的方向,将有助于未来发现与肠道免疫系统稳态相关的问题,并导致针对这些疾病的新治疗策略。

项目成果

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Irving C Allen其他文献

Irving C Allen的其他文献

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{{ truncateString('Irving C Allen', 18)}}的其他基金

Optimization of High Frequency Irreversible Electroporation (H-FIRE) for tumor ablation and immune system activation in pancreatic cancer applications
高频不可逆电穿孔 (H-FIRE) 的优化,用于胰腺癌应用中的肿瘤消融和免疫系统激活
  • 批准号:
    10659581
  • 财政年份:
    2023
  • 资助金额:
    $ 8.05万
  • 项目类别:
Deploying Histotripsy Based Tumor Ablation Strategies to Treat Pancreatic Cancer
部署基于组织解剖学的肿瘤消融策略来治疗胰腺癌
  • 批准号:
    10418848
  • 财政年份:
    2022
  • 资助金额:
    $ 8.05万
  • 项目类别:
Deploying Histotripsy Based Tumor Ablation Strategies to Treat Pancreatic Cancer
部署基于组织解剖学的肿瘤消融策略来治疗胰腺癌
  • 批准号:
    10612053
  • 财政年份:
    2022
  • 资助金额:
    $ 8.05万
  • 项目类别:
Employing Novel Porcine Models of Orthotopic Pancreatic Cancer to Evaluate Histotripsy Based Tumor Ablation Strategies
采用新型猪原位胰腺癌模型来评估基于组织解剖的肿瘤消融策略
  • 批准号:
    9807506
  • 财政年份:
    2019
  • 资助金额:
    $ 8.05万
  • 项目类别:
NLR Regulation of Gastrointestinal Inflammation and Tumorigenesis
NLR 对胃肠道炎症和肿瘤发生的调节
  • 批准号:
    8468173
  • 财政年份:
    2011
  • 资助金额:
    $ 8.05万
  • 项目类别:
NLR Regulation of Gastrointestinal Inflammation and Tumorigenesis
NLR 对胃肠道炎症和肿瘤发生的调节
  • 批准号:
    8165280
  • 财政年份:
    2011
  • 资助金额:
    $ 8.05万
  • 项目类别:
NLR Regulation of Gastrointestinal Inflammation and Tumorigenesis
NLR 对胃肠道炎症和肿瘤发生的调节
  • 批准号:
    8572668
  • 财政年份:
    2011
  • 资助金额:
    $ 8.05万
  • 项目类别:
NLR Regulation of Gastrointestinal Inflammation and Tumorigenesis
NLR 对胃肠道炎症和肿瘤发生的调节
  • 批准号:
    8731867
  • 财政年份:
    2011
  • 资助金额:
    $ 8.05万
  • 项目类别:
NLR Regulation of Gastrointestinal Inflammation and Tumorigenesis
NLR 对胃肠道炎症和肿瘤发生的调节
  • 批准号:
    8331448
  • 财政年份:
    2011
  • 资助金额:
    $ 8.05万
  • 项目类别:

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