Bioengineered Scaffolds for Spinal Cord Injury

用于脊髓损伤的生物工程支架

• 批准号: 8840584
• 负责人: Jeffrey Sakamoto
• 金额: $ 46.22万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-15 至 2016-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8840584
• 关键词: American; Axon; Behavioral; Biodegradation; Biomedical Engineering; Brain-Derived Neurotrophic Factor; Caregivers; Cells; Chemical Engineering; Clinical; Clinical Trials; Development; Drug Delivery Systems; Generations; Grant; Growth Factor; Hydrogels; Implant; Laboratories; Lead; Length; Lesion; Michigan; Modeling; Motor; Nanotechnology; Natural regeneration; Nervous system structure; Neurons; Neurosciences; Patients; Property; Rattus; Recovery; Recovery of Function; Rehabilitation therapy; Reporting; Research; Rodent; Science; Sepharose; Site; Spinal; Spinal Cord; Spinal Cord transection injury; Spinal cord injury; Staging; Stem cells; System; Technology; Testing; Tissue Engineering; Translations; United States National Aeronautics and Space Administration; Universities; Vision; Work; axon growth; axon regeneration; base; behavioral outcome; controlled release; design; economic impact; experience; improved; in vivo; in vivo Model; injured; multimodality; nonhuman primate; psychologic; scaffold; stem cell biology; success

DESCRIPTION (provided by applicant): Approximately 1.2 million Americans have sustained some form of spinal cord injury (SCI), with an estimated annual economic impact of $20 billion. Our overarching vision is to use a multimodality approach to promote axon regeneration and improve behavioral outcomes in the most challenging and clinically predictive SCI model, complete spinal transection. We will optimize and reduce to practicality bioengineered, template agarose guidance scaffolds for axonal regeneration based on substantial experience from a collaborative, multi-PI effort that pairs leaders in bioengineering at Michigan State University with a top national laboratory in the field of SCI research at UC San Diego. Specific Aim 1: Optimize Templated Agarose Scaffold Design Optimize scaffold design by adding: a)drug delivery capabilities using layer by layer (LBL) technology to control release of growth factors, and b) biodegradability properties by integration of hydrogels or agarose/polyelectrolyte composites. Specific Aim 2: UseTemplated Agarose Scaffolds to Promote Host Axonal Regeneration After T3 Complete Spinal Cord Injury Aim 2 will use optimized scaffolds from Aim 1 to enhance axonal growth into and beyond SCI lesions in vivo. Success in this model can lead to clinical translation. Specific Aim 3: Use Templated Agarose Scaffolds to Promote Formation of Neuronal Relay Circuits After T3 Complete Spinal Cord Injury Aim 3 will combine two cutting edge technologies - bioengineered scaffolds and stem cells - to formulate a new generation of therapies that could constitute the most promising approach yet for treating SCI. The natural progression of this work can lead to translation to our non-human primate model of SCI, and then to clinical trials.

描述（由申请人提供）：大约有120万美国人患有某种形式的脊髓损伤（SCI），估计每年的经济影响为200亿美元。 我们的总体愿景是在最具挑战性和临床预测性的SCI模型（完全脊髓横断）中使用多模态方法促进轴突再生并改善行为结果。 我们将优化和减少到实用性生物工程，模板琼脂糖指导支架轴突再生的基础上大量的经验，从合作，多PI的努力，对领导人在生物工程在密歇根州立大学与顶级国家实验室在SCI研究领域在加州大学圣地亚哥分校。 具体目标1：优化模板化琼脂糖支架设计通过增加以下内容优化支架设计：a）使用逐层（LBL）技术控制生长因子释放的药物递送能力，以及B）通过整合水凝胶或琼脂糖/琼脂糖复合材料的生物降解性能。 具体目标二：T3完全脊髓损伤后促进宿主轴突再生的琼脂糖模板支架Aim 2将使用Aim 1的优化支架来增强体内SCI损伤内和损伤外的轴突生长。 这种模式的成功可以导致临床翻译。 具体目标3：使用模板琼脂糖支架促进T3完全性脊髓损伤后神经元中继回路的形成Aim 3将联合收割机结合两种尖端技术-生物工程支架和干细胞-制定新一代的治疗方法，可能构成治疗SCI最有前途的方法。 这项工作的自然进展可以导致我们的非人类灵长类动物SCI模型的翻译，然后进行临床试验。