权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Diffuse illumination in fiber bundle microendoscopy for deep tissue imaging

纤维束显微内窥镜深部组织成像中的漫射照明

基本信息

批准号：
8788926
负责人：
Timothy J Muldoon
金额：
$ 6.78万
依托单位：
UNIVERSITY OF ARKANSAS AT FAYETTEVILLE
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-01-01 至 2015-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8788926
关键词：
Applications Grants Basement membrane Biopsy Cells Cellular Structures Characteristics Clinical Collagen Complement Contrast Media Coupled Data Devices Diagnosis Diagnostic Diffuse Diffuse Pattern Dimensions Distal Dyes Dysplasia Endoscopes Endoscopy Epithelial Epithelium Evaluation Face Fiber Fluorescence Fluorescent Dyes Goals Gold Hand Health Hemoglobin Heterogeneity Image Imaging Techniques In Situ Label Lesion Light Lighting Malignant Neoplasms Maps Measurement Melanins Metals Methods Microscope Modeling Molecular Monte Carlo Method Neoplasms Operative Surgical Procedures Optics Pathway interactions Pattern Penetration Performance Photons Physiological Pilot Projects Procedures Property Publications Resolution Resources Screening for cancer Source Structure Surface Suspension substance Suspensions System Techniques Thick Time Tissues absorption base bioimaging cellular imaging clinical decision-making flexibility fluorescence imaging fluorescence microscope image guided imaging system improved innovation light scattering millimeter molecular imaging nanocage nanomaterials nanoparticle novel optical fiber point of care

项目摘要

DESCRIPTION (provided by applicant): Microendoscopic imaging is rapidly becoming an increasingly important field of biomedical imaging. With the ability to provide clinicians histologc information at the point of care during endoscopic procedures, it will be possible to better target surgical biopsies and improve clinical decision-making. One approach employs a flexible, coherent fiber bundle image guide coupled to a simple fluorescent microscope to generate images when in contact with fluorescently labeled epithelial tissues. A significant limitation of this epi-illumination strategy is lack of penetration depth in highly scattering tissues. In order o improve the diagnostic efficacy of this device, we propose a fiber bundle microendoscope imaging system employing a novel off-axis near infrared illumination fiber; the fiber bundle will be used to image the remitted diffusely reflected light. With the use of well-developed Monte Carlo forward models of photon propagation through tissue, we can effectively predict the pattern of diffuse reflection in three dimensions for a given distribution of absorbers in scatterig tissue. The pattern of diffusely reflected light remitted from the surface of tissue and collected y the image guide bundle can be predicted using this modeling approach. Using absorbing materials, it will also be possible to develop layered optical phantoms with discrete heterogeneities that can be quantitatively analyzed using the modified microendoscope. These optical phantoms will consist of systems of increasing complexity, comprising simple absorbing dyes, tuned NIR absorbed gold nanocages, and labeled cells in a collagen suspension. These phantoms will enable clear comparisons between simulated Monte Carlo estimates of performance, and actual experimental measurements. Imaging of diffusely reflected light will demonstrate the use of optically absorbing contrast agents, including molecule-specific nanomaterials, which have been optimized to absorb in the NIR wavelength range. The proposed imaging system will allow the use of these agents using a simple fiber bundle microendoscope device, and enable interrogation of deep structures within epithelial tissues. We believe this approach will greatly broaden the range of available contrast agents to fiber bundle microendoscopes, and significantly improve the capability of this technique across a range of translational applications.

描述（申请人提供）：显微内镜成像正迅速成为生物医学成像的一个重要领域。在内窥镜手术过程中，有能力在护理点向临床医生提供组织学信息，这将有可能更好地定位