Mouse Resource Core

黄病毒的主要感受器，视黄酸诱导基因1蛋白（RlG-1），黑色素瘤分化 抗原5（MDA 5;也称为1F 1H 1）和LGP 2（也称为DHX 58），在几乎所有的哺乳动物中基本表达。 在体内的所有细胞类型，并在病毒感染期间和响应I型 IFN下游衔接蛋白，线粒体抗病毒信号传导（MAVS）也广泛表达。 这使得很难准确地定义哪些细胞以及何时这些传感器通路对先天免疫至关重要。 免疫或免疫病理学。尽管使用条件性敲入（KI）小鼠的进展， 小鼠的先天免疫信号传导成分要么没有被基因评级，要么没有被广泛使用。 该核心将通过开发和为研究者提供Kl小鼠来帮助研究者，在这些小鼠中， 传感器（RIG-1、MDA 5、LPG 2）、信号传导元件（MAVS、IFNR、IL 28 RA、IL-1 R）或细胞因子（BAFF、IL-IBeta） 在先天免疫细胞如树突状细胞（DC）或巨噬细胞中或在 B细胞、CD 4 T细胞或神经元。核心还将开发一套新颖的报告小鼠， 免疫细胞因子如IFNB、IL-1B和BAFF可在病毒感染后容易地检测到。这个核心很可能 通过提供这些新型小鼠，对先天免疫和病毒免疫学领域产生重大影响 资源

The major sensors of flaviviruses, retinoic acid-inducible gene 1 protein (RlG-1), melanoma differentiation antigen 5 (MDA5; also known as 1F1H1) and LGP2 (also known as DHX58), are expressed basally in nearly all cell types in the body and are induced to increased levels during viral infection and in response to type I IFNs The downstream adaptor protein, mitochondrial antiviral signaling (MAVS) is also broadly expressed. This makes it difficult to define exactly in which cells and when these sensor pathways are critical for innate immunity or immunopathology. In spite of the advances of using conditionally knocked in (Kl) mice, floxed mice for innate immune signaling components either have not been gene rated or have not been used widely. This Core will help investigators by developing and providing investigators with Kl mice in which key viral sensors (RIG-1, MDA5, LPG2), signaling elements (MAVS, IFNR, IL28RA, 1L-1R) or cytokines (BAFF, IL-IBeta) have been selectively knocked out in innate immune cells such as dendritic cells (DC) or macrophages or in B cells, CD4 T cells or neurons. The Core will also develop a set of novel reporter mice so that innate immune cytokines like IFNB, 1L-1B and BAFF can be readily detected after viral infections. This Core is likely to significantly impact the field of innate immunity and viral immunology by providing these novel mouse resources.