Mouse Resource Core

鼠标资源核心

• 批准号: 8811087
• 负责人: Edward A Clark
• 金额: $ 36.75万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-05-01 至 2019-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8811087
• 关键词: Adaptor Signaling Protein; Affect; Antiviral Agents; B-Lymphocytes; Breeding; CD4 Positive T Lymphocytes; Cells; Communities; Confocal Microscopy; Critical Pathways; Dendritic Cells; Differentiation Antigens; Elements; Family; Flavivirus; Flavivirus Infections; Flow Cytometry; Gene Targeting; Genes; Genotype; Goals; Immune; Immune Response Genes; Immune response; Immunology; Infection; Interferon Type I; Interferon-beta1; Interferons; Investigation; Knock-in Mouse; Knock-out; Knockout Mice; Mitochondria; Mus; Natural Immunity; Neurons; Phenotype; Proteins; RNA; Reporter; Reporter Genes; Research; Research Personnel; Resource Sharing; Resources; Signal Transduction; Signaling Molecule; Testing; Transgenic Mice; Tretinoin; Viral; Virus; Virus Diseases; adaptive immunity; cell type; chromophore; cytokine; immunopathology; macrophage; melanoma; member; novel; programs; recombinase; red fluorescent protein; response; sensor

The major sensors of flaviviruses, retinoic acid-inducible gene 1 protein (RlG-1), melanoma differentiation antigen 5 (MDA5; also known as 1F1H1) and LGP2 (also known as DHX58), are expressed basally in nearly all cell types in the body and are induced to increased levels during viral infection and in response to type I IFNs The downstream adaptor protein, mitochondrial antiviral signaling (MAVS) is also broadly expressed. This makes it difficult to define exactly in which cells and when these sensor pathways are critical for innate immunity or immunopathology. In spite of the advances of using conditionally knocked in (Kl) mice, floxed mice for innate immune signaling components either have not been gene rated or have not been used widely. This Core will help investigators by developing and providing investigators with Kl mice in which key viral sensors (RIG-1, MDA5, LPG2), signaling elements (MAVS, IFNR, IL28RA, 1L-1R) or cytokines (BAFF, IL-IBeta) have been selectively knocked out in innate immune cells such as dendritic cells (DC) or macrophages or in B cells, CD4 T cells or neurons. The Core will also develop a set of novel reporter mice so that innate immune cytokines like IFNB, 1L-1B and BAFF can be readily detected after viral infections. This Core is likely to significantly impact the field of innate immunity and viral immunology by providing these novel mouse resources.

黄病毒的主要传感器，视黄酸诱导基因1蛋白（RlG-1），黑色素瘤分化