Development of fluorescence-based receptor binding assays for the detection
开发基于荧光的受体结合检测方法
基本信息
- 批准号:8783099
- 负责人:
- 金额:$ 22.44万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2014
- 资助国家:美国
- 起止时间:2014-08-01 至 2016-01-31
- 项目状态:已结题
- 来源:
- 关键词:AcidsAffinityAgonistAlgaeAnimalsAreaBackBedsBindingBiological AssayCell CountCessation of lifeCiguatoxinsColorCyclophosphamideDetectionDevelopmentDissociationEatingEthical IssuesExtinction (Psychology)FisheriesFishesFluorescenceGeographic stateGoalsHealthHeatingHigh Pressure Liquid ChromatographyHumanInternationalLabelLengthLigandsMammalsMarine ToxinsMarinesMarketingMeasuresMethodsMexicoMonitorMusNeurotoxinsNew EnglandPacific OceanParalysedPhasePhytoplanktonPoisoningProcessProductionPublic HealthRadioactive WasteRadioactivityRadioligand AssayResearchResearch PersonnelResourcesRiskSamplingSanitationSavingsSaxitoxinSeafoodShellfishSmall Business Technology Transfer ResearchSpecificityTechniquesTechnologyTestingTimeToxic effectToxinToxin ConjugatesUnited StatesValidationWorkassay developmentauthoritybasebrevetoxincoastal watercookingcostdomoic acidfeedingfluorophoreharmful algal bloomshealth economicsinnovationkillingsmicroorganismmortalitynovelprogramsprototypepublic health relevanceradioligandreceptorreceptor bindingscreeningsymposiumuser-friendlywater sampling
项目摘要
DESCRIPTION (provided by applicant): Harmful algal blooms (HABs) are becoming frequent occurrences off the coasts of the United States, particularly in coastal waters of New England, the Gulf of Mexico, and the Pacific Ocean. Historically, HABs have been associated with fish kills and marine mammal mortalities; however, their effects on human health and economic loss due to HAB contamination of seafood are becoming more prevalent, with conservative estimates around $82 million annually. The toxic effects of HABs are precipitated by the production of toxins by various species of marine algae and microorganisms, which are then consumed by filter feeding shellfish and finfish. Accumulation in significant quantities can result
in toxicity and death in marine animals and humans. Many of the natural toxins produced by marine phytoplankton are heat and acid stable; therefore, cooking contaminated seafood does not eliminate the risk of poisoning. Currently, US state agencies monitor for the presence of toxic phytoplankton and when the cell count reaches a set level, shellfish beds and finfish are tested for the presence of toxin. When toxin levels reach FDA set limits, fishery resources are closed. However, current methods for detection of marine toxins most commonly associated with seafood poisonings in the US have serious drawbacks, including lengthy assay time, high cost, animal usage, low sensitivity and/or sample throughput, or small working ranges. The purpose of this project is to develop a fluorescence based receptor binding assay (FBA) for the detection of marine neurotoxins that cause paralytic shellfish poisoning (saxitoxin) and amnesic shellfish poisoning (domoic acid). These assays could be used as a rapid test alternative to current methods and they have the advantage of lower cost, high sensitivity and lower animal usage. The FBA will be an improvement over the radioligand receptor binding assay (RBA) for these toxins, as the fluorescence platform does not require the use of radioactivity and is thus safer and far less expensive. Using the same techniques as the FBA that we recently developed for brevetoxins and ciguatoxins, fluorophores will be conjugated to saxitoxin and domoic acid and used as the labeled ligand to examine interactions with the toxins' receptors. As RBAs have been used for a variety of sample matrices and been shown to strongly correlate with the mouse bioassay and HPLC results in samples, FBAs could be the next progressive step in the detection of toxins in seafood or coastal water samples thereby protecting human health and aiding in the monitoring of fishery resources.
描述(由申请人提供):有害藻华(HABs)在美国海岸附近越来越频繁地发生,特别是在新英格兰、墨西哥湾和太平洋的沿海水域。从历史上看,赤潮与鱼类死亡和海洋哺乳动物死亡有关;然而,它们对人类健康的影响和由于藻华污染海产品造成的经济损失正变得越来越普遍,保守估计每年损失约为8200万美元。有害藻华的毒性作用是由各种海洋藻类和微生物产生的毒素引起的,这些毒素随后被滤食性贝类和鱼类消耗。会导致大量的积累
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Jennifer Rettew McCall其他文献
Jennifer Rettew McCall的其他文献
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