Development of High-expressing Ion Channels for Research and Therapeutic Applicat

描述（由申请人提供）：离子通道，如Kv1.3和Nav1.7，与许多人类疾病有关，包括自身免疫和疼痛，每年影响100亿美国人，但治疗不力。然而，由于其结构的复杂性和毒性，离子通道通常特别难以在高水平上表达。因此，在需要高水平膜蛋白的情况下，这些靶点的许多研究和治疗应用都非常有限。例如，对于人电压门控钾（Kv）和钠（Nav）离子通道，没有解决晶体结构，没有治疗性单克隆抗体（mab）被FDA批准，甚至没有临床试验，尽管这类单克隆抗体作为治疗药物具有很高的制药兴趣。这些应用通常都需要高水平的蛋白质表达，而大多数离子通道的表达通常低于成功所需的数量级。在这里，我们建议开发一个表达高水平构象离子通道蛋白的通用平台。我们在本提案范围内使用该平台将用于分离针对Kv1.3和Nav1.7的唯一单克隆抗体。我们希望这个平台可以扩展到其他离子通道，也可以用于结构研究、高通量筛选和生物医学研究试剂。

DESCRIPTION (provided by applicant): Ion channels, such as Kv1.3 and Nav1.7, are involved in a number of human disorders, including autoimmunity and pain that affect >100 million Americans annually and are poorly treated. However, ion channels are typically exceptionally difficult to express at high levels due to their structural complexity and toxicity. s a result, many research and therapeutic applications for these targets have been very limited where high-levels of membrane protein are required. For example, for human voltage-gated potassium (Kv) and sodium (Nav) ion channels, there are no solved crystal structures and no therapeutic monoclonal antibodies (MAbs) approved by the FDA or even in clinical trials, despite the high level of pharmaceutical interest in such MAbs as therapeutics. These applications all typically require high levels of protein expression, and the expression of most ion channels is usually orders of magnitude below what is required for success. Here we propose to develop a versatile platform for expressing high levels of conformational ion channel proteins. Our use of this platform within the scope of this proposal will be for isolating unique MAbs against Kv1.3 and Nav1.7. We expect that this platform will be extensible to other ion channels and will also have utility for structural research, high-throughput screening, and as biomedical research reagents.