OTHER FUNCTION - Cell-Free Synthesis of Site-specific Phosphothreonine Proteins

其他功能 - 位点特异性磷酸苏氨酸蛋白的无细胞合成

• 批准号: 8947530
• 负责人: CHRISTOPHER MURRAY
• 金额: $ 16.84万
• 依托单位: GALEN BIOTECHNOLOGIES, LLC
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-19 至 2015-06-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8947530
• 关键词: Antigens; Biological Assay; Cell physiology; Cells; Contracts; Generations; Goals; Immunoassay; Malignant Neoplasms; Modification; National Cancer Institute; Phospho-Specific Antibodies; Phosphorylation; Phosphothreonine; Play; Production; Protein Biosynthesis; Proteins; Role; Site; Specific qualifier value; Technology; Threonine; design; innovation; kinase inhibitor; polypeptide; protein protein interaction

As phosphorylated proteins play a significant role in normal and abnormal cellular function, there is a critical need for the production of pure, analytically characterized phospho-proteins/polypeptides for use in assays designed to capture the phosphorylation signatures of different cancers. Phospho-protein/polypeptide standards can serve as reference controls for quantitative immunoassays, immunogens for generating phospho-specific antibodies, and can National Cancer Institute (NCI) 65 be utilized in kinase inhibitor screens as well as protein-protein interaction studies.

由于磷酸化蛋白质在正常和异常细胞功能中发挥着重要作用，因此迫切需要生产纯的、经过分析表征的磷酸化蛋白质/多肽，用于旨在捕获不同癌症的磷酸化特征的测定。磷酸化蛋白/多肽标准品可用作定量免疫测定的参考对照、用于生成磷酸化特异性抗体的免疫原，并且可以将国家癌症研究所 (NCI) 65 用于激酶抑制剂筛选以及蛋白质-蛋白质相互作用研究。