权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

High Throughput Single-Cell Phenotype Isolation by Protrusion Analysis Chip (PAC)

通过突出分析芯片 (PAC) 进行高通量单细胞表型分离

基本信息

批准号：
8929634
负责人：
Lidong Qin
金额：
$ 20.81万
依托单位：
METHODIST HOSPITAL RESEARCH INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-08-15 至 2018-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8929634
关键词：
Adhesions Behavior Bioinformatics Biological Biological Assay Biological Markers Breast Cancer Cell Breast Cancer Patient Cancer Patient Cancer cell line Cell Separation Cell Survival Cell physiology Cells Clinical Collection Cost Analysis Data Development Devices Diagnosis Disease Event Filopodia Fluorescence-Activated Cell Sorting Fluorescent in Situ Hybridization Future Gene Expression Gene Expression Profiling Genes Genotype Goals Heterogeneity Label Length MDA MB 231 Malignant - descriptor Malignant Neoplasms Mediating Membrane Methods Mutation Phase Phenotype Play Population Printing Process RNA Sequences Research Retrieval Role Sampling Science Solid Neoplasm Specimen Staging Surface Suspension substance Suspensions Techniques Technology Development Study Testing Time Variant Vial device Work anticancer research base biophysical properties cancer cell cancer diagnosis cancer prevention cancer therapy cell motility migration next generation sequencing personalized medicine prevent public health relevance single cell analysis technological innovation tool trait transcriptome sequencing tumor

项目摘要

DESCRIPTION (provided by applicant): High Throughput Single-Cell Phenotype Isolation by Protrusion Analysis Chip (PAC) There is increasing evidence that solid tumors are likely comprised of many subpopulations of cells with distinct genotypes and phenotypes, which is a phenomenon termed intratumor heterogeneity. Such heterogeneity becomes a major obstacle to effective cancer treatment and personalized medicine. Because of this inherent heterogeneity, data collected from cancer cell population-averaged assays likely hides valuable but rare events such as dramatic variations in gene expression at the single cell level. Therefore, understanding cellular heterogeneity from cancer biospecimens, especially in the study of phenotype-genotype correlation, will facilitate identification of new cell subsets, and assist in cancer prevention, diagnosis, and therapy. In this proposal, we focus on developing a high throughput approach for single-cell isolation based on cells' capability in generating protrusions. To be able to retrieve the desired single adherent cell, a Protrusion Analysis Chip (PAC) is proposed, in which the single cell is captured by a single hook and physically isolated by a barrier. The PAC is rapid, operationally simple, highly efficient, and requires low-volume sample introduction. After adhesion and spreading, cell phenotype is identified microscopically and then the desired cell is retrieved for genotype analysis. The proposed technology developments and study plans may potentially strengthen the understanding of the relationship between phenotype and genotype at the single cell level.

描述（由申请人提供）：通过突出分析芯片（PAC）进行高通量单细胞表型分离越来越多的证据表明实体瘤可能由许多具有不同基因型和表型的细胞亚群组成，这是一种称为肿瘤内异质性的现象。这种异质性成为有效癌症治疗和个性化医疗的主要障碍。由于这种固有的异质性，从癌细胞群体平均检测中收集的数据可能隐藏了有价值但罕见的事件，例如单细胞水平上基因表达的巨大变化。因此，从癌症生物样本中了解细胞异质性，特别是在表型-基因型相关性的研究中，将有助于识别新的细胞亚群，并有助于癌症的预防、诊断和治疗。在本提案中，我们专注于开发一种基于细胞产生突起的能力的高通量单细胞分离方法。为了能够检索所需的单个贴壁细胞，提出了一种突出分析芯片（PAC），其中单个细胞由单个钩捕获并由屏障物理隔离。 PAC 速度快、操作简单、效率高，并且需要少量样品引入。粘附和扩散后，通过显微镜鉴定细胞表型，然后检索所需细胞进行基因型分析。拟议的技术开发和研究计划可能会加强对单细胞水平表型和基因型之间关系的理解。