Sample Processing and Analysis Core

这个核心的目的是为低温冷藏和分配提供集中化的实验室资源 给调查人员编程的病人样本。核心Virtll还维护一个带注释的数据库，该数据库与 每个样本的病理数据，并开发基因组和功能分析，以支持临床研究。 这一核心有4个具体目标： 1.从临床研究方案涉及的患者中获取原发白血病样本，并 分离和保存RNA、DNA和可存活的单个核细胞。 2.维护一个包含病理、细胞遗传学和分子信息的注释数据库 临床标本取自髓系白血病患者。 3.建立一套标准化的基因组和功能分析方法，为临床研究提供支持。 4.维护为体内研究优化的AML细胞库和AML库

The purpose ofthis core Is to provide a cen tialized laboratory resource for cryopresen/ation and distiibution of patient samples to program investigators. The core virtll also maintain an annotated database with relevant pathologic data for each sample and develop genomic and functional assays to support clinical investigation. This core has 4 Specific Aims: 1. To acquire primary leukemia samples from p atients ente red in clinical research protocols and to isolate and preserve RNA, DNA, and viable mononuclear cells. 2. To maintain an annotated database containing pathologic, cytogenetic and molecular infomiation for clinical samples obtained from patients witii myeloid leukemias. 3. To develop a set of standardized genomic and functional assays to support clinteal investigation. 4. To maintain a bank of AML cell lines optimized for in vivo studies and a bank of AML