Crosstalk Between Metabolism and Inflammation in Pulmonary Hypertension

肺动脉高压代谢与炎症之间的串扰

• 批准号: 8800338
• 负责人: Kurt R. Stenmark
• 金额: $ 49.22万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-12-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8800338
• 关键词: Address; Affinity; Animal Model; Animals; Anti-Inflammatory Agents; Anti-inflammatory; Apoptosis; Binding; Blood Vessels; C-Terminal Binding Protein 1; C-terminal binding protein; Cattle; Cells; Chronic; Clinical Management; Complex; DNA Binding; Data; Disease; Environment; Enzymes; Exhibits; Fibroblasts; Figs - dietary; Gene Expression Profile; Genes; Genetic Transcription; Goals; Histones; Human; Hypoxia; IL6 gene; In Vitro; Inflammation; Inflammation Mediators; Inflammatory; Interleukin-10; Investigation; Link; Lung; Macrophage Activation; Malignant Neoplasms; Mediating; Metabolic; Metabolism; Methodology; Molecular; Mus; NADH; Oxidation-Reduction; Pathogenesis; Pathway interactions; Patients; Phenotype; Play; Process; Proteins; Pulmonary Hypertension; Pulmonary artery structure; Reagent; Recruitment Activity; Repression; Research; Resistance; Rodent; Role; STAT3 gene; Sensory; Signal Induction; Signal Transduction; Syndrome; Testing; Therapeutic Intervention; Transcription Repressor/Corepressor; Tunica Adventitia; Vascular Diseases; Vascular remodeling; aerobic glycolysis; cancer cell; cell behavior; cell type; chemokine; cytokine; design; histone modification; hypoxia inducible factor 1; in vivo; innovation; macrophage; novel; prevent; public health relevance; response; sensor; transcription factor

DESCRIPTION (provided by applicant): Inflammatory processes are increasingly appreciated to play a critical role in vascular remodeling. This proposal addresses the role of inflammation, and specifically the role of macrophages in the vascular remodeling that characterize hypoxic forms of pulmonary hypertension (PH). Observations in most forms of PH show that macrophages accumulate primarily in the adventitial and perivascular regions of the vessel. Strong preliminary data demonstrate that fibroblasts resident in the adventitia of PH vessels undergo significant metabolic changes leading to increases in intracellular NADH concentration and subsequent activation of the transcriptional repressor C-terminal binding protein 1 (CtBP1), a transcriptional redox sensor with high binding affinity for NADH. CtBP1 acts specifically to repress expression of anti-inflammatory genes including, hemoxygenase-1 (HMOX-1). These "activated" fibroblasts recruit and activate macrophages toward a pro- inflammatory/remodeling phenotype largely through IL6, STAT3, HIF1 signaling and induction of aerobic glycolysis. We propose that both the pro-inflammatory fibroblast and fibroblast activated macrophage phenotype in hypoxic PH is driven by CtBP1. During hypoxia and/or in conditions of aerobic glycolysis we have shown that NADH-activated CtBP1 acts as a transcriptional co-repressor and complexes with DNA-binding transcription factors and histone modification enzymes (HDACs, HMTs). CtBP1 therefore links the metabolic status of the cell to specific coordination of gene transcription and, as we have shown, exerts a dominant role in determining cell behavior. We now address the novel hypothesis that CtBP1 drives the pro-inflammatory functions of fibroblasts in PH, leading to fibroblast-mediated macrophage activation. The inflammatory environment, caused by the interaction of fibroblasts and macrophages, leads to PH and pulmonary vascular remodeling. Our approach relies on in vitro and in vivo studies using lung adventitial fibroblasts and primary na�ve macrophages from hypoxic cows, rodents, and human controls and PH patients, and novel genetically modified mice. AIM 1: Determine the role of CtBP1 in "locking" the fibroblast into a pro-inflammatory phenotype. AIM 2: Determine the role of CtBP1 in controlling the pro-inflammatory/pro-remodeling phenotype of fibroblast-activated macrophages. AIM 3: Determine whether CtBP1/HO-1 signaling can be targeted in a cell-type-specific manner in vivo to prevent or even reverse hypoxia-induced PH. Our investigations will ultimately provide key data in elucidating the role of inflammation as a cause/contributor, a bystander, or whether or when it is simply the end result of the disease process. This is a critica step to advance our understanding of chronic pulmonary vascular disease in order to significantly impact the clinical management of PH.

描述（由申请人提供）：炎症过程在血管重塑中扮演着重要的角色。本研究旨在探讨炎症的作用，特别是巨噬细胞在缺氧型肺动脉高压（PH）血管重构中的作用。在大多数形式的PH中观察到，巨噬细胞主要积聚在血管的外膜和血管周围区域。强有力的初步数据表明，居住在PH血管外膜的成纤维细胞发生显著的代谢变化，导致细胞内NADH浓度增加，随后激活转录抑制因子c -末端结合蛋白1 (CtBP1)，这是一种对NADH具有高结合亲和力的转录氧化还原传感器。CtBP1特异性抑制抗炎基因的表达，包括血氧合酶-1 （HMOX-1）。这些被“激活”的成纤维细胞通过IL6、STAT3、HIF1信号和诱导有氧糖酵解来招募和激活巨噬细胞，使其向促炎症/重塑表型发展。我们提出促炎成纤维细胞和成纤维细胞活化的巨噬细胞表型在缺氧PH下都是由CtBP1驱动的。在缺氧和/或有氧糖酵解条件下，我们已经证明nadh激活的CtBP1作为转录共抑制因子并与dna结合转录因子和组蛋白修饰酶（hdac, hmt）复合物。因此，CtBP1将细胞的代谢状态与基因转录的特定协调联系起来，并且如我们所示，在决定细胞行为方面发挥主导作用。我们现在提出了一个新的假设，即CtBP1驱动PH中成纤维细胞的促炎功能，导致成纤维细胞介导的巨噬细胞活化。由成纤维细胞和巨噬细胞相互作用引起的炎症环境导致PH和肺血管重塑。我们的方法依赖于体外和体内研究，使用来自缺氧奶牛、啮齿动物、人类对照和PH患者以及新型转基因小鼠的肺内膜成纤维细胞和原生巨噬细胞。目的1：确定CtBP1在将成纤维细胞“锁定”为促炎表型中的作用。目的2：确定CtBP1在控制成纤维细胞活化的巨噬细胞的促炎/促重塑表型中的作用。目的3：确定CtBP1/HO-1信号是否可以在体内以细胞类型特异性的方式靶向，以预防甚至逆转缺氧诱导的ph。我们的研究将最终提供关键数据，以阐明炎症作为原因/贡献者，旁观者，或者是否或何时仅仅是疾病过程的最终结果。这是促进我们对慢性肺血管疾病理解的关键一步，从而对PH的临床管理产生重大影响。