An integrated X-ray data collection system

 描述(由申请人提供)：1999年，阿尔伯特·爱因斯坦医学院成功地获得了名为“集成X射线图像平板探测器系统”的共享仪器补助金(1S10RR013658-01；Almo，PI)。在过去的14年里，该系统一直在持续使用，并支持着一个非常广泛和富有成效的结构生物学社区。阿尔伯特·爱因斯坦医学院现在正在申请资金，以购买最先进的X射线数据收集系统，包括MicroMax“-007 HF旋转阳极发生器、VariMax”光学元件、AFC11四轴测角仪、Dectris Pilatus P200K探测器、牛津冷冻系统Cryostream和相关外围设备。这种仪器满足了四个关键需求：1)我们现有的设备已经老化到无法保证维修和更换部件的程度；2)需要增强内部数据收集能力，以支持学院的众多结构生物学项目；3)由于国家同步加速器光源(NSLS)即将关闭，需要寻找替代数据收集机会，国家同步加速器光源是学院整个结构生物学社区的主要数据收集场所；以及4)需要满足日益增长的院校教育使命。MicroMax“-007的相当大的流量与Pilatus P200K探测器的光子计数能力相结合，使数据采集率比我们现有系统提供的数据采集率高两个数量级。这一改进为内部数据收集提供了新的机会，这将显著影响阿尔伯特·爱因斯坦医学院许多结构生物学项目的起诉效率和速度。这些能力将使纽约地区5所机构(爱因斯坦、布鲁克林CUNY、洛克菲勒、霍夫斯特拉和布鲁克海文国家实验室)10个系(爱因斯坦生物化学、解剖学和结构生物学、生理学和生物物理学、医学、微生物学和免疫学以及神经科学；附近研究所的4个系)的24个实验室的研究计划受益。这些计划涵盖了广泛的尖端生物医学领域，包括人类免疫、癌症、自身免疫、传染病、大分子组装、功能和调节、过渡态分析、药物发现、RNA生物学、表观遗传学、功能注释、环境科学和系统生物学。所要求的X射线数据收集系统将产生广泛的科学影响，对于阿尔伯特·爱因斯坦医学院和邻近机构结构生物学的持续活力和发展至关重要。

 DESCRIPTION (provided by applicant): In 1999 the Albert Einstein College of Medicine successfully competed for a Shared Instrumentation Grant entitled "Integrated X-ray Image Plate Detector System" (1S10RR013658-01; Almo, PI). This system has been in continuous use and supported an enormously broad and productive structural biology community for the past ~14 years. The Albert Einstein College of Medicine is now requesting funds to purchase a state-of-the-art X-ray data collection system, including a MicroMax"-007 HF rotating anode generator, a VariMax" optic, AFC11 4-axis goniometer, Dectris Pilatus P200K detector, Oxford Cryosystems Cryostream and associated peripherals. This instrumentation fills four critical needs: 1) our existing equipment has aged to the point that service and replacement parts can no longer be guaranteed; 2) the need for enhanced in-house data collection capabilities to support the numerous structural biology programs at the College; 3) the need to identify alternative data collection opportunities due to the imminent closure of the National Synchrotron Light Source (NSLS), which is the major data collection venue for the entire structural biology community at the College; and 4) the need to meet the growing institutional educational mission. The considerable flux of the MicroMax"-007 coupled with the photon counting capabilities of the Pilatus P200K detector results in data collection rates that are two orders of magnitude greater than those afforded by our existing system. This enhancement provides new opportunities for in-house data collection that will significantly impact the efficiency and rate at which the many structural biology programs are prosecuted at the Albert Einstein College of Medicine. These capabilities will benefit the research programs of 24 laboratories in 10 departments (Einstein Biochemistry, Anatomy & Structural Biology, Physiology & Biophysics, Medicine, Microbiology & Immunology, and Neuroscience; 4 departments at nearby institutes) at 5 New York area institutions (Einstein, Brooklyn CUNY, Rockefeller, Hofstra, and Brookhaven National Laboratory). These programs span a wide range of cutting-edge biomedical areas, including human immunity, cancer, autoimmunity, infectious disease, macromolecular assembly, function and regulation, transition state analysis, drug discovery, RNA biology, epigenetics, functional annotation, environmental science and systems biology. The requested X-ray data collection system will have broad scientific impact and is essential for the continued vigor and development of structural biology at the Albert Einstein College of Medicine and neighboring institutions.