Spatiotemporal Integration of Mechanical Information in the Rat Brainstem

大鼠脑干机械信息的时空整合

• 批准号: 8909521
• 负责人: Nicholas Edward Bush
• 金额: $ 3.67万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-05-01 至 2018-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8909521
• 关键词: Air; Animals; Area; Behavior; Brain; Brain Stem; Cell Nucleus; Cells; Characteristics; Complex; Data; Exhibits; Glare; Inferior; Investigation; Knowledge; Lead; Mechanics; Medial; Modality; Modeling; Monitor; Motion; Motivation; Neurons; Olives - dietary; Output; Pathway interactions; Pattern; Physiological; Pontine structure; Positioning Attribute; Preparation; Property; Rattus; Reporting; Rest; Role; Running; Sensory; Sensory Process; Septal Region; Series; Shapes; Signal Transduction; Speed; Staging; Stimulus; Structure; Structure of trigeminal nerve spinal tract nucleus; Surface; Tactile; Testing; Thalamic structure; Time; Touch sensation; Trigeminal System; Vibrissae; Video Recording; Work; awake; base; public health relevance; receptive field; relating to nervous system; research study; response; sensory integration; spatiotemporal; superior colliculus Corpora quadrigemina; zona incerta

 DESCRIPTION (provided by applicant): Early stages of sensory processing responsible for the integration of tactile information across multiple whiskers remains poorly understood despite frequent reports of thalamic and cortical multiwhisker responses. Here we propose to fill two major gaps in the characterization of the synthesis of multiple-whisker receptive field by recording from projection neurons in the rostral region of Spinal Trigeminal Nucleus Interpolaris (SpVir)-a brainstem region which receives primary afferent input from multiple whiskers. First, there has been no characterization of SpVir neuron responses to quantified mechanical stimuli. Second, only one study reported recording from these neurons in the awake animal and this study did not attempt to characterize these neurons. The work proposed here will fill both these gaps. First, to characterize these cells' responses to carefully quantified spatial and temporal mechanical inputs, we will record from SpVir neurons in the anaesthetized animal while presenting a variety of mechanical stimuli to the whiskers. Simultaneously, we will record high-speed video from multiple angles to extract the 3D shape of the whiskers during contact. This will allow us to quantify contact timing and force inputs to all whiskers and compare to neural recordings. Second, we will record from neurons in SpVir while an awake animal performs a series of localization tasks. During awake, naturalistic exploration, the patterns of contact with objects change drastically and top down modulatory inputs may alter receptive field structure. We will record from multiple single neurons simultaneously while the rat performs localization tasks with varying physical stimuli. Using multiple angle high speed video recordings we will determine the patterns of contact during naturalistic investigation of different objects. We will then compare the contact patterns with neural spiking to determine the spatial and temporal structure of the receptive field during awake exploration. These experiments promise to fill the two glaring gaps in our understanding of spatiotemporal integration of tactile information: what are the precise relationships between mechanical input and spiking output in these cells; and what general characteristics do these cells exhibit in the awake, behaving animal.