Flow Cytometry and Cell Separation Shared Resource (FC-SR)

流式细胞术和细胞分离共享资源 (FC-SR)

• 批准号: 8855797
• 负责人: JAMES F. LEARY
• 金额: $ 1.91万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8855797
• 关键词: Ablation; Biology; Cancer Center Support Grant; Cell Separation; Cells; Characteristics; Client; Cytometry; Data; Data Analyses; Development; Dimensions; Equipment; Flow Cytometry; Fluorescence; Funding; Grant; Human; Human Resources; Image; Image Analysis; Lasers; Licensing; Life; Malignant Neoplasms; Measures; Mus; NCI Center for Cancer Research; Optics; Organelles; Peer Review; Property; Research; Research Personnel; Research Project Grants; Resource Sharing; Resources; Scanning; Services; Site; Sorting - Cell Movement; Technical Expertise; Training; United States National Institutes of Health; Universities; analytical tool; anticancer research; cell analyzer; cost effective; design; experience; instrument; member; particle; programs; research study

Flow Cytometry and Cell Separation Shared Resource: Project Summary Biology Shared Resource Group The Purdue Flow Cytometry and Cell Separation Shared Resource (FC-SR) provides advanced cell and particle analysis, and sorting capabilities to Purdue University Center for Cancer Research (PCCR) members. As an analytical tool, flow cytometry allows users to identify and quantitate cellular features, organelles, or structural components using optical measures. When combined with sorting capabilities, it is possible to identify and isolate single cells that have specific properties or express specific molecules and which can then be further examined and characterized. In cancer research, where the identification and characterization of unique, specific cells with cancer-like characteristics is critical, flow cytometry and cell sorting are extremely vital and useful to today's cancer researcher. Purdue's FC-SR provides a broad range of services that supported 24 Purdue Center for Cancer Research (PCCR) investigators' research projects from all four of PCCR's Research Programs in this current year. The FC-SR, located in the Bindley Bioscience Center (BBC) of Discovery Park, has grown considerably in both its technical expertise and instrument capabilities since its total re-organization in March 2009. A major emphasis of the facility is live cell sorting of mouse and human cells under BSL-2 conditions. In 2010 with support from an NIH S10 Shared Instrument Grant, the FC-SR obtained a high-throughput LEAP image scanning cytometer. This instrument adds an additional dimension to sorting of attached cells by laser ablation and serves as a bridge between flow cytometry and image analysis capabilities. The FC-SR also operates a FACS Aria III Sorter that serves as the primary workhorse for analysis and cell sorting. Currently, the facilty also operates a FC500 Cell Analyzer for routine cell analyses. The data analysis capabilities of the facility have advanced greatly with the addition of many FlowJo site licenses and the development of new advanced data analysis capabilities. Perhaps the most important resource provided by the FC-SR is the expertise that the staff shares. Many of FC-SR's clients have little, if any, experience conducting cytometry analysis or designing experiments using the BD FACS Aria III cell sorter. In the absence of the FC-SR and its experienced staff, these investigators would have tremendous difficulty in generating cytometry data necessary for competitive external funding.

流式细胞术和细胞分离共享资源： 项目摘要 生物共享资源群 普渡流式细胞术和细胞分离共享资源(FC-SR)提供高级细胞和 为普渡大学癌症研究中心(PCCR)成员提供粒子分析和分类能力。 作为一种分析工具，流式细胞术允许用户识别和定量细胞特征、细胞器或 使用光学测量的结构组件。当与排序功能相结合时，可以 鉴定和分离具有特定性质或表达特定分子的单个细胞，然后这些细胞可以 有待进一步研究和表征。在癌症研究中，识别和表征 具有肿瘤样特征的独特的、特定的细胞至关重要，流式细胞术和细胞分选非常重要 对今天的癌症研究人员来说是至关重要和有用的。普渡的FC-SR提供广泛的服务， 支持普渡癌症研究中心(PCCR)研究人员的24个研究项目，来自所有四个 PCCR今年的研究计划。 FC-SR位于探索公园的宾德利生物科学中心(BBC)，在 自2009年3月全面重组以来，其技术专长和仪器能力都得到了提高。一位少校 该设施的重点是在BSL-2条件下对小鼠和人类细胞进行活细胞分离。在2010年， 在NIH S10共享仪器拨款的支持下，FC-SR获得了高通量的LEAP映像 扫描细胞仪。该仪器为激光消融对附着细胞的分选增加了一个额外的维度 并作为流式细胞术和图像分析能力之间的桥梁。FC-SR还运行 FACS Aria III分选器，是分析和细胞分选的主要工具。目前，设施 还使用FC500细胞分析仪进行常规细胞分析。该设施的数据分析能力具有 通过添加许多FlowJo站点许可证和开发新的高级数据，实现了巨大的进步 分析能力。 也许FC-SR提供的最重要的资源是工作人员共享的专业知识。许多. FC-SR的客户几乎没有进行细胞分析或设计实验的经验 BD FACS Aria III细胞分选机。在FC-SR及其经验丰富的工作人员缺席的情况下，这些调查人员 在产生竞争性外部资金所需的细胞学数据方面将会有极大的困难。