Microbial Discovery and Immunity in ME/CFS

ME/CFS 中的微生物发现和免疫

• 批准号: 9102389
• 负责人: MADY HORNIG
• 金额: $ 76.62万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-14 至 2017-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9102389
• 关键词: Abbreviations; Advocate; Age; Animal Model; Antibodies; Arthralgia; Bacteria; Biological Assay; Biomedical Research; Blood; Borna disease virus; Borrelia burgdorferi; Candida albicans; Chronic Fatigue Syndrome; Clinical; Cognition; Communities; Coxiella burnetii; Data; Databases; Development; Diagnosis; Diagnostic; Diagnostic tests; Disease; Ensure; Enterovirus; Etiology; Family; Fatigue; Feces; Foundations; Future; Geography; Headache; Health; High-Throughput Nucleotide Sequencing; Human Herpesvirus 4; Human Herpesvirus 6; Immune; Immunity; Infection; Infectious Agent; Infectious Disease Epidemiology; Intervention; Laboratories; Lead; Link; Lower Gastrointestinal Tract; Lymphatic Diseases; Medical; Medical Care Costs; Memory; Methods; Microbe; Multicenter Studies; Myalgia; Mycoplasma pneumoniae; National Institute of Allergy and Infectious Disease; Night Sweating; Nucleic Acids; Nucleotides; Oral; Oropharyngeal; Patient advocacy; Patients; Peripheral Blood Mononuclear Cell; Physical Examination; Plasma; Population; Positioning Attribute; Prevalence; Prevention; Recording of previous events; Recruitment Activity; Reporting; Research; Research Personnel; Resource Allocation; Retroviridae; Ribosomal RNA; Risk; Sampling; Sequence Analysis; Serologic tests; Serological; Site; Socioeconomic Status; Sore Throat; Surveys; Swab; Symptoms; Testing; Therapeutic Intervention; Time; Translational Research; Viral; Virus; Work; case control; cost; experience; follow-up; fungus; microbial; pathogen; prevent; repository; sample collection; sex; virome

 DESCRIPTION (provided by applicant): Myalgic Encephalomyelitis/Chronic Fatigue Syndrome is an idiopathic disabling disorder characterized by persistent, unexplained fatigue in association with impaired memory or cognition, muscle or joint pain, headache, sore throat, tender lymphadenopathy and night sweats. The prevalence in the US is estimated at over 200 cases/100,000 population with annual medical care costs of $24 billion. There is no specific diagnostic test or treatment. Although the majority of cases are sporadic, there are reports of geographic and temporal clusters of CFS and many subjects report a viral prodrome and symptoms consistent with an infection. Efforts to identify causative agents have yielded inconsistent results and in some instances culminated in false leads that polarized the patient advocate and biomedical research communities, led to inappropriate allocation of resources and placed patients at risk for inappropriate therapeutic interventions. This project will test the hypothesis that CFS cases and controls differ with respect to bacterial, fungal or viral microflora in the oropharynx, lower gastrointestinal tract and blood in a well-powered study, using rigorously characterized cases and controls and state-of-the-art methods for microbial surveillance and discovery. Our proposal builds on the foundation established during the NIAID CFS multicenter study of XMRV/pMLV. It includes the expert clinicians who led work at the original six sites, thereby ensuring credibility within the scientific and CFS patient and advocacy communities and access to a network within which follow-up studies and translational research can be readily pursued. The laboratory team has extensive experience in infectious disease epidemiology, microbial discovery and de-discovery, as well as in the development of serological assays and animal models needed to test for causal relationships and investigate pathogenetic mechanisms.

 描述（由申请方提供）：肌痛性脑脊髓炎/慢性疲劳综合征是一种特发性致残性疾病，其特征为持续性、原因不明的疲劳，伴有记忆或认知受损、肌肉或关节疼痛、头痛、咽喉痛、压痛性淋巴结病和盗汗。美国的患病率估计超过200例/100，000人，每年的医疗保健费用为240亿美元。没有特定的诊断测试或治疗。虽然大多数病例是散发性的，但也有CFS的地理和时间集群的报告，许多受试者报告了与感染一致的病毒前驱症状和症状。努力确定病原体产生了不一致的结果，在某些情况下，最终导致错误的线索，使患者的倡导者和生物医学研究界两极分化，导致资源分配不当，并使患者处于不适当的治疗干预的风险之中。本项目将检验CFS病例和对照组在细菌、真菌或病毒菌群方面不同的假设 在一项有力的研究中，使用严格表征的病例和对照以及最先进的微生物监测和发现方法，我们的建议建立在NIAID CFS对XMRV/pMLV进行多中心研究的基础上。它包括在最初的六个地点领导工作的专家临床医生，从而确保科学和CFS患者和宣传的可信度。 社区和进入网络，在其中可以随时进行后续研究和转化研究。该实验室团队在传染病流行病学、微生物发现和去发现以及开发测试因果关系和调查致病机制所需的血清学测定和动物模型方面拥有丰富的经验。