Protein and Small Molecule Chemistry

项目总结/摘要：核心C 蛋白质和小分子化学核心的作用是中央的所有研究人员的需求， 计划项目提案。核心将为项目研究人员提供纯化的蛋白质和病毒样物质， 颗粒（VLP）以及小分子和肽的定制合成。核心还将建造 根据研究者的需要，通过定点诱变获得突变形式的蛋白质。最后，核心将 表征和定量蛋白质-蛋白质和蛋白质-配体相互作用。核心活动的例子包括 纯化诺如病毒蛋白酶，以促进药物发现和VLP的纯化，用于研究 抗体与衣壳的相互作用。活性还包括合成小分子蛋白酶抑制剂 生物化学和基于细胞的测试以及结构研究的候选人。此外，核心将 测量小分子与诺如病毒蛋白酶的结合亲和力以及抗体-VLP的亲和力 蛋白质相互作用和其他蛋白质-蛋白质相互作用。最后，核心将 根据需要在诺如病毒蛋白酶、p22和p41基因以及其他基因中构建定点突变， 项目调查员的功能研究。核心人员在方法方面具有丰富的经验 提供这些服务所需的所有设备和资源， 核心提供蛋白质纯化和小分子合成、诱变、 核心设施中的约束性测量避免了单个调查人员获取 这是一种有效的手段，可促进在实现 该项目

PROJECT SUMMARY/ABSTRACT: Core C The role of the Protein and Small Molecule Chemistry Core is central to the needs of all the investigators in the Program Project Proposal. The Core will provide project investigators access to purified proteins and virus-like particle (VLPs) as well as custom synthesis of small molecules and peptides. The Core will also construct mutant versions of proteins by site-directed mutagenesis as needed by investigators. Finally, the Core will characterize and quantitate protein-protein and protein-ligand interactions. Examples of Core activities include purification of norovirus protease to facilitate drug discovery and purification of VLPs for investigations of antibody interactions with capsid. Activities also include synthesis of small molecule protease inhibitor candidates for biochemical and cell-based testing as well as structural studies. In addition, the Core will measure binding affinity of small molecules with norovirus protease as well as affinity of antibody-VLP interactions and other protein-protein interactions as needed by project investigators. Finally, the Core will construct site-directed mutations in the norovirus protease, p22, and p41 genes and others as needed for functional studies by project investigators. The Core personnel have extensive experience in the methods required for providing these services and all of the equipment and resources necessary are available in the Core. The performance of services such as protein purification and small molecule synthesis, mutagenesis, and binding measurements in a Core facility avoids the need for individual investigators to acquire the necessary expertise and equipment and is an efficient means of facilitating progress on the scientific goals of the project.