The flagellar motor as a sensor

作为传感器的鞭毛马达

• 批准号: 8916804
• 负责人: Rasika M Harshey
• 金额: $ 27.86万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2016-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8916804
• 关键词: Bacteria; Bacterial Infections; Behavior; Binding; Binding Sites; Cells; Chemicals; Chemotaxis; Complex; Environment; Escherichia coli; Experimental Designs; Film; Flagella; Health; Knowledge; Lead; Light; Liquid substance; Locales; Microbial Biofilms; Modeling; Molecular; Molecular Conformation; Monitor; Motor; Organelles; Output; Pathogenesis; Pathway interactions; Play; Power Sources; Property; Proteins; Protons; Recruitment Activity; Role; Rotation; Running; Salmonella; Sensory; Signal Transduction; Signaling Molecule; Solid; Speed; Surface; Swimming; Testing; Transcriptional Activation; Variant; base; cell motility; insight; microbial community; migration; receptor; research study; response; sensor

DESCRIPTION (provided by applicant): The Flagellar Motor as a Sensor Bacteria use rotation of helical flagella to propel themselves either through bulk liquid (swimming), or through a thin film of liquid on a solid surface (swarming). Chemosensory pathways normally communicate environmental information to the bidirectional rotary motor, modulating its CW/CCW bias to optimize bacterial migration towards favorable locales. These pathways are well-understood. In E. coli and other bacteria, the motor is also itself a sensory organelle, allowing bacteria to adapt to chemical signals, respond directly to increased c-di- GMP, and mechanically 'sense' a solid surface. These pathways, particularly mechanosensing, are not understood. In light of recent evidence showing that the motor restructures its stator and rotor components during both chemosensing and mechanosensing, we propose a new testable model for how the motor acts as a mechanosensor. The model identifies the rotor-stator interface as the sensory hub of the motor. We consider a finite number of structural components that could receive and transduce sensory signals from this hub. These are: MotA/B, FliG, FliM, FliL, YcgR and H-NS. The first two aims of this proposal investigate remodeling of a subset of these components under situations where the motor elicits a distinct response to two internal signals - CheY~P and c-di-GMP. The third aim benefits from this knowledge to monitor remodeling of these components in response to mechano-signals. The insights derived from these studies are expected to eventually trace the input signal(s) at the motor to output responses that promote either motility or sessility (biofilms), both of which play critical roles i bacterial infection, surface colonization, pathogenesis, and persistence.

描述（由申请人提供）：鞭毛马达作为传感器细菌利用螺旋鞭毛的旋转推动自己通过散装液体（游泳）或通过液体

项目成果

Swarming bacteria migrate by Lévy Walk.

• DOI: 10.1038/ncomms9396
• 发表时间: 2015-09-25
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Ariel G;Rabani A;Benisty S;Partridge JD;Harshey RM;Be'er A
• 通讯作者: Be'er A