Animal Models, Stem Cells and Cell Therapy

动物模型、干细胞和细胞治疗核心通过以节省成本的方式提供独特的资源、技术和科学专业知识，促进马里恩·贝辛肝脏研究中心的翻译研究。自上一次中心更新以来，对核心进行了重组，保留了以前的所有服务，并增加了服务，以应对中心调查人员不断变化的需求。Core在尖端技术和资源方面积累了广泛的专业知识，用于使用包括干细胞在内的各种肝细胞类型以及独特的动物模型进行研究。这有利于中心研究人员从事病理生理机制或细胞和基因治疗的基础研究。例如，中心研究人员在疾病模型、高质量细胞制剂的分配方面的专业知识为中心研究人员创造了令人兴奋的机会，这些制剂有助于代谢缺陷状态、遗传疾病、辐射损伤和急性或慢性肝功能衰竭的新治疗开发。与此同时，在肝脏基因表达、干/祖细胞生物学、炎症、自噬、肝生长调控、肝运输的病理生理机制等方面的研究也取得了进展。同样，教育活动定位于核心 作为一种重要的资源。这一核心的目标如下： 繁育、饲养和提供其他来源难以获得的动物， 开发或复制相关的诱导动物模型。 为了分离和分布高质量的原代动物或人类肝细胞， “为了使用高效的方案提供由多能干细胞产生的肝细胞， “指导研究人员使用干细胞方法和常用的动物程序， 为动物研究的实验设计提供咨询 为了提供优化的、成本效益高的肝细胞培养条件和添加剂， “为了整合动物和人类干细胞及细胞治疗研究的新技术， 鼓励早期研究人员或肝脏研究新手使用Core。

The Animal Models, Stem Cells and Cell Therapy Core facilitates translational research in the Marion Bessin Liver Research Center by providing unique resources, technologies and scientific expertise at cost savings. The Core was reorganized since previous the Center renewal, preserving all previous services and including additional services in response to the evolving needs of Center Investigators. The Core has accumulated extensive expertise in cutting-edge technologies and resources for studies using various liver cell types, including stem cells, as well as unique animal models. This benefits Center Investigators pursuing basic studies of pathophysiological mechanisms or cell and gene therapy. For instance, exciting opportunities are generated for Center Investigators through expertise in disease models, distribution of high quality cell preparations, which were instrumental for novel therapeutic developments in metabolic deficiency states, genetic diseases, radiation injury and acute or chronic liver failure. Simultaneously, numerous studies were advanced in hepatic gene expression, stem/progenitor cell biology, inflammation, autophagy, liver growth control, pathophysiological mechanisms of liver transport. Similarly, educational activities position the Core as an important resource. The Aims of this Core are as follows: To breed, maintain and provide animals that are not readily available from other sources, " To develop or reproduce relevant induced animal models. To isolate and distribute high quality primary animal or human liver cells, " To provide liver cells generated from pluripotent stem cells using highly efficient protocols, " To instruct investigators in stem cell methods and commonly used animal procedures, " To offer consultation for experimental design for animal studies To provide optimized, cost-effective liver cell culture conditions and additives, " To incorporate new technologies for animal and human stem cell and cell therapy studies, " To encourage use of the Core by early-stage investigators or investigators new to liver research.