Animal Models, Stem Cells and Cell Therapy

动物模型、干细胞和细胞疗法

• 批准号: 9133724
• 负责人: SANJEEV GUPTA
• 金额: $ 23.64万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE, INC
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2019-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9133724
• 关键词: Acute; Animal Model; Animals; Attention; Autophagocytosis; Breeding; Cell Culture Techniques; Cell Therapy; Cellular biology; Chronic; Consultations; Cost Savings; Development; Disease; Disease model; Educational Activities; Experimental Designs; Gene Expression; Generations; Growth; Hepatic; Hepatocyte; Hepatolenticular Degeneration; Hereditary Disease; Human; Inflammation; Liver; Liver Failure; Liver diseases; Metabolic; Metabolic Diseases; Methods; Pluripotent Stem Cells; Positioning Attribute; Procedures; Protocols documentation; Radiation Injuries; Rare Diseases; Research; Research Personnel; Resources; Services; Source; Staging; Technology; Therapeutic Studies; Translational Research; Viral hepatitis; cell preparation; cell type; cost effective; gene therapy; human stem cells; insight; new technology; novel; novel diagnostics; novel therapeutics; response; stem; stem cells; therapeutic development; tool

The Animal Models, Stem Cells and Cell Therapy Core facilitates translational research in the Marion Bessin Liver Research Center by providing unique resources, technologies and scientific expertise at cost savings. The Core was reorganized since previous the Center renewal, preserving all previous services and including additional services in response to the evolving needs of Center Investigators. The Core has accumulated extensive expertise in cutting-edge technologies and resources for studies using various liver cell types, including stem cells, as well as unique animal models. This benefits Center Investigators pursuing basic studies of pathophysiological mechanisms or cell and gene therapy. For instance, exciting opportunities are generated for Center Investigators through expertise in disease models, distribution of high quality cell preparations, which were instrumental for novel therapeutic developments in metabolic deficiency states, genetic diseases, radiation injury and acute or chronic liver failure. Simultaneously, numerous studies were advanced in hepatic gene expression, stem/progenitor cell biology, inflammation, autophagy, liver growth control, pathophysiological mechanisms of liver transport. Similarly, educational activities position the Core as an important resource. The Aims of this Core are as follows: To breed, maintain and provide animals that are not readily available from other sources, " To develop or reproduce relevant induced animal models. To isolate and distribute high quality primary animal or human liver cells, " To provide liver cells generated from pluripotent stem cells using highly efficient protocols, " To instruct investigators in stem cell methods and commonly used animal procedures, " To offer consultation for experimental design for animal studies To provide optimized, cost-effective liver cell culture conditions and additives, " To incorporate new technologies for animal and human stem cell and cell therapy studies, " To encourage use of the Core by early-stage investigators or investigators new to liver research.

动物模型，干细胞和细胞治疗核心通过提供独特的资源，技术和科学专业知识，以节省成本，促进Marion Bessin肝脏研究中心的转化研究。自上一次中心更新以来，对核心进行了重组，保留了所有先前的服务，并纳入了额外的服务，以应对中心研究者不断变化的需求。The Core在尖端技术和资源方面积累了广泛的专业知识，用于使用各种肝细胞类型（包括干细胞）以及独特的动物模型进行研究。这有利于从事病理生理机制或细胞和基因治疗基础研究的中心研究人员。例如，通过疾病模型方面的专业知识、高质量细胞制剂的分发，为中心研究人员创造了令人兴奋的机会，这对于代谢缺陷状态、遗传性疾病、辐射损伤和急性或慢性肝衰竭的新型治疗开发至关重要。与此同时，在肝脏基因表达、干/祖细胞生物学、炎症、自噬、肝脏生长控制、肝脏转运的病理生理机制等方面也有大量的研究进展。同样，教育活动将核心 作为重要的资源。该核心的目标如下： 繁殖、饲养和提供不易从其他来源获得的动物， “开发或复制相关的诱导动物模型。 为了分离和分配高质量的原代动物或人肝细胞， “为了提供使用高效方案从多能干细胞产生的肝细胞， “为了指导研究人员使用干细胞方法和常用的动物程序， the results show that this method can be used to determine the effects of different methods on the effects of different methods on the effects of different methods.“ 为了提供优化的、具有成本效益的肝细胞培养条件和添加剂， “为了将新技术用于动物和人类干细胞和细胞治疗研究， “鼓励早期研究者或肝脏研究新手使用核心。