Molecular Cell and Analyitical Services Facility Core

分子细胞和分析服务设施核心

• 批准号: 9040176
• 负责人: Max Costa
• 金额: $ 14.98万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2017-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9040176
• 关键词: Air Pollution; Apoptosis; Bioinformatics; Biological Assay; Candidate Disease Gene; Carcinogens; Cell Cycle; Cells; Cellular biology; Consultations; Core Facility; Culture Media; DNA; DNA Methylation; DNA Sequence; Data; Disease; Documentation; Dose; Education; Educational workshop; Emerging Technologies; Environment; Environmental Exposure; Environmental Health; Epigenetic Process; Equipment; Evolution; Flow Cytometry; Fluorescence; Fluorescence Resonance Energy Transfer; Funding; Funding Agency; Gel; Gene Expression; Genes; Genome; Genomics; Health; Housing; Human; Human Genome; Imagery; Immune response; Metals; Methodology; Methylation; Microscopy; Molecular; Molecular Biology; Molecular Biology Techniques; Organism; Ozone; Particulate; Pathway Analysis; Pathway interactions; Play; Preparation; Preventive Intervention; Reagent; Research; Research Personnel; Research Support; Resources; Roentgen Rays; Role; Sentinel; Services; Signal Pathway; Signal Transduction; Site; Techniques; Technology; Testing; Therapeutic Intervention; Time; Tissues; Toxic Environmental Substances; Training; Training and Education; Work; Yeasts; absorption; biomarker discovery; cost efficient; design; discount; environmental agent; environmental stressor; epigenetic regulation; epigenome; epigenomics; fetal bovine serum; gene environment interaction; instrument; interdisciplinary approach; mammalian genome; member; predictive marker; protein expression; pyrosequencing; research study; response; statistics; tissue culture; tool; transcriptome; transcriptome sequencing; trend; whole genome

The overall objective of the Molecular Cell and Analytical Services Facility Core (MCASFC) is to provide Center members with education on, and access to, state-of-the-art tools for assessing the effects of the environment on human and mammalian genomes and epigenomes. The Core also provides analytical services, particularly those for metals analysis in cells and tissues of exposed organisms, since metal effects on the genome and epigenome are specialized research strengths of the Center. The rationale for, and continuing evolution of, this Core reflects current trends in environmental health research, and in particular the rapidly changing field of gene x environment interactions. Functionally important, dose-related perturbations to human health from environmental stressors (e.g. metals, carcinogens, air pollution particulates) are being actively analyzed by Center researchers who study changes in gene expression, epigenetic regulation at candidate gene and whole genome levels, cell cycle perturbations, protein expression and signaling pathway interactions. The MCASFC supports Center investigators' funded studies using genomics and epigenomics to identify biomarkers and predictive sentinels of toxic challenges to health, as well as to identify targets for preventive and therapeutic interventions. One new initiative implemented in 2008 and overseen by this core is the collaborative yeast screen project involving analysis of global gene expression changes induced by metals and ozone. This new project, supported in part by the Center Director's fund, involved multiple Center investigators with Dr. Thomas Begley at the GeNYsis Center in Albany, NY. It has become increasingly evident that the epigenome plays an enormous role in modulating the responses of organisms to environmental exposures. Thus, other new initiatives that have developed for this Core since the last renewal are the incorporation of ChlP-Chip, ChlP-Seq and RNA-Seq for epigenetic and transcriptome biomarker discovery and for investigating mechanisms underlying toxic and disease responses to environmental agents. Research on genome/epigenome x environment interactions requires multidisciplinary approaches and utilization of highly sensitive molecular techniques supported by state-of-the-art instruments and emerging technologies. The MCASFC provides Center investigators with evolving access to specialized methodologies and equipment, either on-site or off-site, by taking advantage of regional resources involving intra- and interuniversity research partnerships. The MCASFC acquires new equipment as deemed necessary, by continuing to leverage funds from competitive and institutional funding sources. Furthermore, since genome/epigenome studies require considerable training prior to implementation, the Facility Core provides Center investigators with consultation services on the uses, applications, limitations and challenges ofthe latest techniques.

分子细胞和分析服务设施核心（MCASFC）的总体目标是提供 中心成员接受教育，并获得最先进的工具，用于评估 环境对人类和哺乳动物基因组和表观基因组的影响。核心还提供分析 服务，特别是对暴露生物体的细胞和组织中的金属进行分析的服务， 基因组和表观基因组是该中心的专业研究优势。理由，和 该核心的不断演变反映了环境健康研究的当前趋势，特别是 快速变化的基因与环境相互作用领域。功能重要、剂量相关干扰， 环境压力（如金属、致癌物质、空气污染颗粒）对人类健康的影响正在 中心的研究人员积极分析，他们研究基因表达的变化， 候选基因和全基因组水平、细胞周期扰动、蛋白质表达和信号通路 交互. MCASFC支持中心研究人员资助的使用基因组学和表观基因组学的研究， 确定生物标志物和对健康的有毒挑战的预测性哨兵，以及确定目标， 预防和治疗干预。2008年实施并由该核心监督的一项新举措是 协作酵母筛选项目涉及分析金属诱导的全球基因表达变化 和臭氧。这个新项目，部分由中心主任的基金支持，涉及多个中心 研究人员与托马斯贝格利博士在纽约州奥尔巴尼的GeNYsis中心。已变得越来越 显然，表观基因组在调节生物体对 环境暴露。因此，自上次更新以来， 是ChIP-Chip、ChIP-Seq和RNA-Seq的掺入，用于表观遗传和转录组生物标志物 发现和调查机制潜在的毒性和疾病的反应，环境因素。 基因组/表观基因组x环境相互作用的研究需要多学科的方法， 利用高灵敏度的分子技术，由最先进的仪器和新兴的 技术. MCASFC为中心研究人员提供不断发展的专业方法 和设备，无论是现场或场外，通过利用区域资源，涉及内部和大学间 研究伙伴关系。MCASFC在认为必要时购买新设备， 从竞争性和机构资金来源筹集资金。由于基因组/表观基因组 研究在实施前需要大量培训，设施核心为中心研究者提供 就最新技术的使用、应用、局限性和挑战提供咨询服务。