Novel single cell assay for quantitative analysis of cell heterogeneity by noninvasive probing of molecular composition of specific organelles in individual cells

通过无创探测单个细胞中特定细胞器的分子组成来定量分析细胞异质性的新型单细胞测定

• 批准号: 8976966
• 负责人: Andrey Kuzmin
• 金额: $ 14.81万
• 依托单位: ADVANCED CYTOMETRY INSTRUMENTATION SYS
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-01 至 2016-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8976966
• 关键词: Algorithms; Architecture; Biological Assay; Calibration; Cell Nucleolus; Cell physiology; Cells; Classification; Clinical; Computer software; Concentration measurement; Cytometry; DNA; Data; Data Set; Databases; Detection; Development; Equilibrium; Eukaryotic Cell; Fluorescence; Gene Expression; Goals; Heterogeneity; Individual; Label; Laboratories; Lasers; Life; Lipids; Measurement; Measures; Metabolic; Methods; Microscope; Mitochondria; Modification; Molecular; Molecular Probes; Molecular Profiling; Monitor; Nanotechnology; Organelles; Physiological; Power Sources; Proteins; Proteomics; Protocols documentation; RNA; Raman Spectrum Analysis; Regulatory Pathway; Resolution; Secondary Protein Structure; Signal Transduction; Site; Small Business Innovation Research Grant; Spectrometry; System; Techniques; Technology; Time; Validation; Variant; Work; base; cell behavior; commercialization; cost; design; instrumentation; interest; macromolecule; metabolomics; miniaturize; molecular marker; next generation; novel; novel marker; phase 1 study; photonics; public health relevance; research and development; response; single cell analysis; tool; transcriptomics; user-friendly

 DESCRIPTION (provided by applicant): Novel single cell assay for quantitative analysis of cell heterogeneity by noninvasive probing of molecular composition of specific organelles in individual cells Project Summary This current SBIR application in response to PA-13-140, is submitted by Advanced Cytometry Instrumentation Systems (ACIS), a company founded in 2001 to commercialize cutting-edge technologies via R&D in the fields of nanotechnology, photonics, and bio photonics. The current proposal is specifically focused on development and commercialization of next generation single cell assay for quantitative analysis of heterogeneity of eukaryotic cells by noninvasive probing of molecular composition in individual cells and/or specific organelles. This technology will enable real-time monitoring of the concentrations of major classes of macromolecules (proteins, lipids, DNA, RNA) as well secondary structures of proteins in regions of interests in live cells. The technology proposed here integrates (i) highly sensitive, high 3D resolution, low phototoxic, miniaturized and robust confocal Raman micro- spectrometry tool, designed to work in both academic and clinical laboratory and (ii) novel concept of user-friendly Biomolecular Component Analysis (BCA) of measured data sets to generate time-sequenced biomolecular composition profiles in individual cells. Based on the above goals the following specific aims are proposed for this Phase I study: Aim 1: Optimization of confocal Raman micro-spectrometer design for multi-cycle long- term measurements of individual eukaryotic cells. Aim 2: Software and database development for semi-automatic Biomolecular Component Analysis. Aim 3: Validation of the proposed micro-Raman-BCA technology for single cell analysis and quantitative characterization of cellular heterogeneity.



项目成果

Ramanomics: New Omics Disciplines Using Micro Raman Spectrometry with Biomolecular Component Analysis for Molecular Profiling of Biological Structures.

• DOI: 10.3390/bios7040052
• 发表时间: 2017-11-15
• 期刊: Biosensors
• 作者: Kuzmin AN;Pliss A;Prasad PN
• 通讯作者: Prasad PN

Macromolecular Profiling of Organelles in Normal Diploid and Cancer Cells.

• DOI: 10.1021/acs.analchem.7b02822
• 发表时间: 2017-10-17
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Levchenko SM;Kuzmin AN;Pliss A;Qu J;Prasad PN
• 通讯作者: Prasad PN

Molecular profiling of single organelles for quantitative analysis of cellular heterogeneity.

• DOI: 10.1038/s41598-017-06936-z
• 发表时间: 2017-07-26
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Kuzmin AN;Levchenko SM;Pliss A;Qu J;Prasad PN
• 通讯作者: Prasad PN