Biochemistry

项目摘要 该愿景研究核心(VRC)的总体目标是提供： ·获得个人R01奖励范围以外的资源 ·获得单一实验室范围以外的技术专长 ·员工培训，以消除高效翻译研究和协作的障碍 VRC支持的研究领域涵盖视网膜变性的分析和治疗， 发育障碍、青光眼和其他障碍，以及一系列尖端的基本 科学倡议。我们已经实现了四个资源模块，它们延续了 这个研究小组是如何合作的，为拥有21个NEI R01奖项的15名研究人员服务。 生物化学模块从基因测序服务向真正的蛋白质组学(ULTRAN- 三分离，凝胶定量)和代谢组学资源(专门的GC-MS，LC-MS，表面平台- MON共振)更准确地反映了我们目前的研究优势。 具体地说，生化模块提供： ·基于主力机器Beckman XL-90的超速离心法 ·免疫印迹和杂交印迹的红外成像和定量 ·高容量Western blotting和ISH筛查； ·用于电化学发光和放射性检测的X射线film ·新生儿体内DNA构建体电穿孔进入细胞 ·小分子(维甲酸、类胡萝卜素、脂褐素成分、脂肪酸)的高效液相色谱-气相色谱/质谱仪 ·利用SPR生物传感器技术进行蛋白质/配体相互作用的定量 ·培训和全面的数据归档。

Project Summary The OVERALL aims of this Vision Research Core (VRC) are to provide: · access to resources outside the scope of individual R01 awards · access to technical expertise outside the scope a single laboratory · staff training to remove barriers to efficient translational research and collaboration The research areas supported by the VRC span the analysis and treatments of retinal degenerations, developmental disorders, glaucoma and other disorders, as well as and a range of cutting-edge basic science initiatives. We have implemented four resource modules that continue the natural evolution of how this research group works together, serving 15 investigators holding 21 NEI R01 awards. The Biochemistry Module evolved from a gene sequencing service towards a true proteomics (ultracen- trifugation, gel quantitation) and metabolomics resource (specialized GC-MS, HPLC-MS, surface plas- mon resonance) more accurately reflecting our current research strengths. Specifically the Biochemistry Module provides: · Ultracentrifugation based on a workhorse Beckman XL-90 · IR imaging and quantitatiion of immunoblots and hybridization blots · High-capacity Western blotting and ISH screening; · X-ray film developing for ECL and radioactivity detection · Neonatal in vivo electroporation of DNA constructs into cells · HPLC-GC/MS of small molecules (retinoids, carotenoids, lipofuscin components, fatty acids) · Protein/ligand interaction quantitation with SPR biosensor technologies · training and comprehensive data archiving.