University of Utah Core Vision Research Grant

项目摘要 该视觉研究核心（VRC）的总体目标是提供： ·获得个人PI及其R 01奖励范围之外的资源 ·获得单一实验室范围之外的技术专长 ·员工培训，以消除有效转化研究和合作的障碍 ·VRC实验室之间的合作计划 VRC支持的研究领域涵盖视网膜变性的分析和治疗， 发展障碍，青光眼和其他疾病，以及一系列尖端的基础科学 举措我们已经实现了四个资源模块，继续自然演变， 研究小组一起工作，为17名研究人员提供服务，持有22个NEI R 01奖项。 成像模块提供一系列成像（TEM、共焦、扫描光学）和计算服务 （成像，数据库，数学）基于核心实验室的优势和卓越的传统， UU计算机学院，其后代创立了Adobe Systems，Silicon Graphics，Netscape和Pixar。 这些小组之间的合作已经改变了TEM和共焦成像的软件工具。 细胞生物学模块源于莫兰眼科中心和高等医学中心在小鼠遗传学方面的优势。 组织分析的需求。几乎每个JMEC实验室都需要高质量的冷冻切片， 对位，小鼠基因分型，免疫染色训练，免疫细胞化学，定量PCR，原位 杂交技术 . 生理学模块是VRC教师用于动物模型验证的一套强大的工具， 疾病分析：ERG设置（LKC Big Shot和较旧的LKC设置），多焦ERG（Phoenix），体内/体内 体外双光子成像、OptoMotry和Rotarod行为测试，以及一系列眼成像工具（OCT， 微米）。 生物化学模块从基因测序/引物合成服务发展成为真正的 蛋白质组学更准确地反映了我们目前的研究优势（超浓缩，制备 离心、Odyssey定量免疫印迹、凝胶定量）和代谢组学资源（专业 GC-MS、HPLC-MS、表面等离子体共振和蛋白质-配体相互作用）。 行政模块为自愿退休委员会提供专业的赠款管理。

Project Summary The OVERALL aims of this Vision Research Core (VRC) are to provide: • access to resources outside the scope of individual PIs and their R01 awards • access to technical expertise outside the scope a single laboratory • staff training to remove barriers to efficient translational research and collaboration • collaboration initiatives among VRC labs The research areas supported by the VRC span the analysis and treatments of retinal degenerations, developmental disorders, glaucoma and other disorders, as well as a range of cutting-edge basic science initiatives. We have implemented four resource modules that continue the natural evolution of how this research group works together, serving 17 investigators holding 22 NEI R01 awards. The Imaging Module provides a range of imaging (TEM, confocal, scanning optical) and computing services (imaging, database, mathematics) based on strengths of core laboratories and the tradition of excellence of the UU School of Computing, whose descendants founded Adobe Systems, Silicon Graphics, Netscape and Pixar. Collaborations among these groups have transformed software tools for TEM and confocal imaging. The Cell Biology Module derives from the strengths in mouse genetics at the Moran Eye Center and the high demand for tissue profiling. Almost every JMEC laboratory has need of high quality cryosectioning, paratoming, mouse genotyping, immunostaining training, immunocytochemistry, quantitative PCR, in-situ hybridization technologies. . The Physiology Module is a powerful set of tools that VRC faculty use for animal model validation and disease profiling: ERG set-ups (LKC Big Shot and an older LKC setup), multi-focal ERG (Phoenix), in vivo / in vitro 2-photon imaging, OptoMotry and Rotarod behavioral testing, and a range of ocular imaging tools (OCT, Micron). The Biochemistry Module evolved from a gene sequencing/primer synthesis service towards a true proteomics more accurately reflecting our current research strengths (ultracentrifugation, preparative centrifugation, Odyssey quantitative immunoblotting, gel quantitation) and metabolomics resource (specialized GC-MS, HPLC-MS, surface plasmon resonance and protein-ligand interactions). The Administrative Module provides professional grants management for the VRC.