Structured illumination multispectral fluorescence lifetime microendoscopy to detect oral premalignancies

结构照明多光谱荧光寿命显微内窥镜检测口腔癌前病变

• 批准号: 8976837
• 负责人: Bilal Malik
• 金额: $ 7.43万
• 依托单位: TEXAS ENGINEERING EXPERIMENT STATION
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-12-02 至 2018-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8976837
• 关键词: Accounting; American; American Cancer Society; Basal Cell; Benign; Biochemical; Biological; Biological Markers; Carcinoma; Cervical; Characteristics; Cheek structure; Collagen; Collection; Colorectal; Detection; Development; Devices; Diagnosis; Diagnostic; Disease; Drops; Early identification; Endoscopes; Endoscopy; Epithelial; Epithelium; Evaluation; Exhibits; Family suidae; Fluorescence; Generations; Goals; Hamsters; Health; Image; Imaging Techniques; In Vitro; Keratin; Lamina Propria; Lesion; Light; Lighting; Malignant - descriptor; Malignant Neoplasms; Metabolic; Modeling; Modification; Molecular; NADH; Optics; Oral; Oral Examination; Oral cavity; Outcome; Patients; Performance; Pharyngeal structure; Physiological; Porphyrins; Premalignant; Process; Reporting; Research; Screening for Oral Cancer; Sensitivity and Specificity; Signal Transduction; Site; Specificity; Speed; Squamous cell carcinoma; Staging; Structural Genes; Structure; Survival Rate; System; Techniques; Testing; Three-Dimensional Image; Tissue imaging; Tissues; United States; Work; adaptive optics; attenuation; base; carcinogenesis; cofactor; design; fluorophore; imaging system; improved; in vivo; instrument; intraepithelial; liquid crystal; malignant mouth neoplasm; molecular marker; optical imaging; oral carcinogenesis; oral lesion; oral premalignancy; screening; tissue phantom

 DESCRIPTION (provided by applicant): The American Cancer Society estimates that more than 40,000 new cases of invasive cancer in the oral cavity and pharynx are reported each year in the US, and five-year survival rates remain below 40% for advanced stage disease. The goal of this research is to design, build and test a structured illumination-enabled multispectral widefield fluorescence lifetime imaging microendoscope for detection of oral precancer in vivo. Combining structured illumination (SI) with fluorescence lifetime imaging (FLIM) would enable generation of high fidelity three dimensional volumes of the fluorescence intensity and lifetime of the epithelial tissue. We believe that the ability to collect 'optical sections' across the epithelum would allow characterization of tissue biochemical composition due to differential distribution of molecular markers, such as structural proteins and metabolic cofactors, within tissue and will ultimately aid in high sensitivity and specificity detection of early oral cancer development. The specific aims of this proposal are to: 1) construct a structured illumination multispectral FLIM endoscope (SI-FLIM) system to provide optical depth sectioning; 2) characterize the performance and optimize the design specifications of the SI-FLIM endoscope by imaging in vitro and ex vivo; and 3) validate and demonstrate the capability of SI-FLIM by imaging tissue in vivo. The successful completion of this work will enable the development of the first multispectral SI-FLIM microendoscope with the ability to selectively image specific depths within the tissue, and will demonstrate its potential for non-invasive characterization of oral premalignant and malignant lesions. With the wealth of specific molecular information encoded in the collected fluorescence intensity and lifetime in multiple spectral channels, such a device can serve to provide an objective screening of the oral cavity in addition to the clinician's subjective evaluation. Although the proposed endoscope is designed for application of this device in the oral cavity, the SI-FLIM system can be applicable to detect and diagnose other carcinomas (e.g. basal cell, colorectal, and cervical) as well with slight modifications to the endoscope.



项目成果

Volumetric structured illumination microscopy enabled by a tunable-focus lens.

• DOI: 10.1364/ol.40.004943
• 发表时间: 2015-11-01
• 期刊: Optics letters
• 影响因子: 3.6
• 作者: Hinsdale T;Malik BH;Olsovsky C;Jo JA;Maitland KC
• 通讯作者: Maitland KC

Objective Detection of Oral Carcinoma with Multispectral Fluorescence Lifetime Imaging In Vivo.

• DOI: 10.1111/php.12627
• 发表时间: 2016-09
• 期刊: Photochemistry and photobiology
• 影响因子: 3.3
• 作者: Malik BH;Lee J;Cheng S;Cuenca R;Jabbour JM;Cheng YS;Wright JM;Ahmed B;Maitland KC;Jo JA
• 通讯作者: Jo JA