Rapid Identification of Active Agents and Metabolomics of Botanical Supplements

植物补充剂活性成分和代谢组学的快速鉴定

• 批准号: 9652301
• 负责人: RICHARD B VAN BREEMEN
• 金额: $ 9.08万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-01 至 2018-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9652301
• 关键词: Rapid; Identification; Active; Agents; Metabolomics

RFA-AT-11-001; Mechanistic Research on CAM Natural Products (R01) Title: Rapid Identification of Active Agents and Metabolomics of Botanical Supplements Summary: This application addresses the unmet need for high-throughput methods to identify active components inbotanical dietary supplements and to determine their mechanisms of action through metabolomics studies. Ultrafiltration mass spectrometry (PUF-MS), which was invented in this laboratory, is more efficient than conventional bioassay-guided fractionation for the screening, characterization, isolation, and identification of pharmacologically significant ligands contained in botanical dietary supplements or other natural product mixtures. We propose to enhance the productivity of this technology 100-fold using a new ultrafast PUF-MS (UPUF-MS) approach and to develop new validated assays for the standardization of botanical dietary supplements based on UHPLC-MS-MS that are at least 10-fold faster than previously possible. We will incorporate UHPLC-MS into metabolomics studies of archived serum and urine samples from clinical trials of the effects of botanical dietary supplements black cohosh, red clover, hops, Prempro, and placebo on hot flashes in menopausal women. It is our hypothesis that this metabolomics approach will facilitate the discovery of biomarkers indicative of vasomotor symptoms such as hot flashes and night sweats. Then, these biomarkers may be used for the objective, quantitative determination of the effects of botanical dietary supplements on hot flashes instead of relying on subjective self reporting of hot flashes by subjects in clinical trials. Overall, these studies will address the central hypothesis that botanical dietary supplements contain pharmacologically active compounds that can be identified using UPUF-MS, used for chemical standardization with UHPLC-MS-MS and that the in vivo effects of these active constituents can be detected using UHPLC-MS-MS in combination with metabolomics.

rfa - - 11 - 001;CAM天然产物机理研究（R01）

项目成果

Ion mobility-mass spectrometry for the separation and analysis of procyanidins.

• DOI: 10.1002/jms.4377
• 发表时间: 2020-02
• 期刊: Journal of mass spectrometry : JMS
• 作者: Rue EA;Glinski JA;Glinski VB;van Breemen RB
• 通讯作者: van Breemen RB

Drug discovery from natural products using affinity selection-mass spectrometry.

• DOI: 10.1016/j.ddtec.2021.10.005
• 发表时间: 2021-12
• 期刊: Drug discovery today. Technologies
• 作者: Muchiri RN;van Breemen RB
• 通讯作者: van Breemen RB