2D-HPLC-Tandem Mass Spectrometer for Proteomics

用于蛋白质组学的 2D-HPLC-串联质谱仪

• 批准号: 6876925
• 负责人: RICHARD B VAN BREEMEN
• 金额: $ 38.3万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-15 至 2007-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876925
• 关键词: biomedical equipment purchase; electrospray ionization mass spectrometry; high performance liquid chromatography; liquid chromatography mass spectrometry; proteomics

DESCRIPTION (provided by applicant): A 2-dimensional (2D) microcapillary HPLC-tandem mass spectrometer (2D mu LC-MS/MS) will be purchased and used to support the research of a multidisciplinary group of 10 major NIH-funded projects (including several multi-investigator program projects) and 5 minor users. Approximately 80% of the instrument time will be utilized by NIH-funded major users, and the remaining 20% of the time will be used by the minor users and new users from on campus or in the Chicago area. This instrument will complement the MALDI TOF mass spectrometer that is already devoted to proteomics research within the Research Resources Center (RRC) of the University of Illinois at Chicago and will provide unique microcapillary HPLC capabilities and on-line solution phase protein characterization and identification. In particular this instrument will be used by many NIH-funded investigators for proteomic profiling using techniques such as isotope-coded affinity tags (ICAT). Presently, our investigators must seek collaborators at other institutions for such studies, since there are no comparable proteomics instruments available on campus. The proposed instrument will be located on the medical campus of the University of Illinois at Chicago in recently renovated space under the supervision of the P.I. Access to the instrument by new users on and off campus will be assured through the administrative oversight of an advisory committee appointed by the RRC which is a centralized campus instrumentation facility that has been in operation for over 50 years. The RRC recently hired a Ph.D. level senior mass spectrometrist to oversee the MALDI TOP mass spectrometer, and a new proteomics mass spectrometrist will be recruited to operate and maintain the proposed 2D LC-MS/MS system and carry out ICAT and similar analyses. These positions are committed for at least the useful lifetimes of these proteomics instruments. Furthermore, the Mass Spectrometry Laboratory is under the oversight of the P.I. Dr. Richard B. van Breemen, who has more than 20 years of experience in biomedical mass spectrometry. These on-going personnel commitments represent substantial support by the RRC and the university. User fees (determined by the advisory committee) will be collected to defray the costs of consumable supplies and repairs. Overall, the proposed 2D mu LC-MS/MS instrument will complement the existing proteomics resources on campus and will enhance the current research of NIH-funded users by providing analytical capabilities currently unavailable on campus.

描述(由申请人提供)：将购买一台二维(2D)微毛细管高效液相色谱-串联质谱仪(2D MU LC-MS/MS)，并用于支持由10个NIH资助的主要项目(包括几个多研究者计划项目)和5个小用户组成的多学科小组的研究。大约80%的仪器时间将由NIH资助的主要用户使用，其余20%的时间将由校园内或芝加哥地区的次要用户和新用户使用。该仪器将补充已经在芝加哥伊利诺伊大学研究资源中心(RRC)内致力于蛋白质组学研究的MALDI TOF质谱仪，并将提供独特的微毛细管高效液相色谱功能和在线溶液相蛋白质表征和鉴定。特别是，许多由美国国立卫生研究院资助的研究人员将使用这种仪器，利用同位素编码亲和标签(ICAT)等技术进行蛋白质组学分析。目前，我们的研究人员必须在其他机构寻找合作伙伴进行此类研究，因为校园内没有类似的蛋白质组学工具可用。拟议的仪器将设在伊利诺伊大学芝加哥分校最近翻修的医疗园区内，在P.I.的监督下。校园内外的新用户将通过RRC任命的咨询委员会的行政监督来确保使用该仪器，RRC是一个已经运行了50多年的集中式校园仪器设施。RRC最近聘请了一名博士级别的高级质谱仪来监督MALDI TOP质谱仪，并将招聘一名新的蛋白质组质谱师来操作和维护拟议的2D LC-MS/MS系统，并进行ICAT和类似的分析。这些立场至少在这些蛋白质组学工具的有用寿命内有效。此外，质谱学实验室由P.I.理查德·B·范·布里曼博士监督，他在生物医学质谱学方面拥有20多年的经验。这些持续的人员承诺代表了RRC和大学的大力支持。将收取使用费(由咨询委员会确定)，以支付消耗品用品和维修费用。总体而言，拟议的2D MU LC-MS/MS仪器将补充校园内现有的蛋白质组学资源，并将通过提供目前校园内无法提供的分析能力，加强NIH资助用户的当前研究。