TOPOGRAPHY, CONSTITUENTS, AND TOXICITY OF WATERPIPE TOBACCO SMOKE UNDER REALISTIC CONDITIONS

摘要 在西方，水烟斗吸烟作为一种行为/娱乐/社交活动越来越受欢迎 国家。几乎没有监管科学可以告知食品和药物管理局(FDA)关于水管的信息 烟草对健康的影响。目前，缺乏对水烟的适当监管政策。 根据地形描述这些产品的实际使用和由此产生的健康影响的数据， 化学成分和毒理学研究。在此，我们的多学科团队建议确定排放和 水烟吸入剂量，并评价其对肺毒性的影响。我们建议确定 不同的地形会影响吸入成分的水平，从而导致肺部中毒。 我们将测试这样一种假设，即测量用户的吹气地形产生的机器排放将 现实条件下与常规条件下水烟烟雾对肺部毒性的影响 抽烟。 为了验证这一假设，我们设计了三个具体目标： (1)根据自然环境确定吸水烟者的地形和毒性； (2)根据水管测定用户在实际使用条件下吸入的化学成分 冒烟的地形；以及 (3)测定水管烟雾对小鼠肺毒性和遗传毒性的影响。 由这项提案产生的研究结果预计将提供科学基础，以告知 FDA关于水烟产品的制造、分销和营销的规定 保护公众健康。

SUMMARY The popularity of waterpipe smoking as a behavioral/recreational/social activity is increasing in western countries. Little regulatory science is available to inform the Food and Drug Administration (FDA) on waterpipe tobacco health effects. Appropriate regulatory policy for waterpipe tobacco is currently hindered by the lack of data describing realistic use of these products and the resulting health impacts based on topography, constituents, and toxicology research. Herein, our multidisciplinary team proposes to determine emissions and dosages of waterpipe smoke, and evaluate the effects on pulmonary toxicity. We propose to determine the different topographies that can affect the levels of inhaled constituents, thereby causing pulmonary toxicities. We will test the hypothesis that measures of users' puffing topography generated machine emissions will affect pulmonary toxicities by waterpipe tobacco smoke under realistic conditions versus conventional smoking. To test this hypothesis, we designed three specific aims: (1) determine waterpipe smoking topography and toxicity in users based on naturalistic environment; (2) determine the chemical constituents inhaled by users under realistic usage conditions based on waterpipe smoking topography; and (3) determine the impact of waterpipe smoke on pulmonary toxicity and genotoxicity. The research findings generated from this proposal are expected to provide scientific underpinnings to inform the FDA on the regulation of the manufacture, distribution, and marketing of waterpipe tobacco products to protect public health.