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Modeling Neural Development Using Human iPSCs from TSC Patients

使用 TSC 患者的人类 iPSC 进行神经发育建模

基本信息

批准号：
8878367
负责人：
Timothy M Gomez
金额：
$ 18.81万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-07-01 至 2018-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8878367
关键词：
Animal Model Animals Autistic Disorder Axon Behavior Biological Assay Biological Models Biological Neural Networks Cell Line Cells Child Complex Cues Data Defect Dendrites Development Disease Environment Exhibits Fibroblasts Fragile X Syndrome Future Genes Growth Cones Hamartoma Health Hereditary Disease Human In Vitro Individual Knockout Mice Location Mediating Methods Modeling Molecular Morphogenesis Mutation Nervous system structure Neurons Pathway interactions Patients Pediatric Hospitals Pilot Projects Population Preclinical Drug Evaluation Protein Biosynthesis Proteins Regulation Research Personnel Resources Retinal Ganglion Cells Role Sampling Signal Transduction Site Skin Somatic Cell Source Symptoms Synapses TSC1 gene TSC1/2 gene TSC2 gene Testing Thalamic structure Therapeutic Therapeutic Intervention Tuberous sclerosis protein complex Universities Wisconsin Work autism spectrum disorder axon guidance base cell growth cell motility cell type developmental disease experience human TSC1 protein human TSC2 protein human embryonic stem cell induced pluripotent stem cell innovation mTOR protein migration mutant nervous system development neurodevelopment neuron development neuronal growth pluripotency protein function response

项目摘要

DESCRIPTION (provided by applicant): The development of the nervous system requires the proper differentiation, migration and morphogenesis of neurons. The morphological differentiation of individual neurons and assembly of the trillions of neuronal connections that compose the human nervous system occurs through guided extension of axons and dendrites. Molecular guidance cues in the environment of developing neurons guide neuronal growth cones at the tips of extending axons and dendrites. mTOR-mediated local synthesis of new proteins within growth cones as emerged as an important mechanism that controls axon guidance. Mutations in genes involved local protein synthesis are responsible for several human autism spectrum disorders, including Fragile X syndrome and Tuberous Sclerosis Complex (TSC). While modulation of mTOR-dependent protein synthesis is known to be required downstream of both attractive and repulsive axon guidance in several animal model systems, it is unknown if similar mechanisms function in developing human neurons. This proposal will first test whether human Retinal Ganglion Cells (RGCs) derived from human induced pluripotent stem cells (hiPSCs) use mTOR-mediated protein synthesis to respond to positive and negative axon guidance cues. In Aim 2 we will test the role of the TSC1/TSC2 complex, which is a key upstream negative regulator of mTOR. For this we will generate new lines of hiPSCs by reprograming fibroblast cells from patients with TSC. While this proposal will focus on RGCs, a wide variety of other cell and neuronal types can be studied using these new hiPSC lines. Therefore, these important new cell lines will be a valuable resource for many investigators and will be made available for distribution through WiCell. In Aim 3 we will test whether RGCs derived from TSC hiPSCs exhibit abnormal response to axon guidance cues tested in Aim 1.

描述(申请人提供)：神经系统的发展需要神经元的适当分化、迁移和形态发生。单个神经元的形态分化和组成人类神经系统的数万亿个神经元连接的组装，是通过轴突和树突的引导延伸发生的。发育中的神经元环境中的分子引导线索引导延伸轴突和树突顶端的神经元生长锥体。MTOR介导的生长锥体内新蛋白质的局部合成是控制轴突引导的重要机制。涉及局部蛋白质合成的基因突变导致了几种人类自闭症谱系疾病，包括脆性X综合征和结节性硬化症(TSC)。虽然已知在几个动物模型系统中，吸引和排斥轴突引导的下游都需要调节mTOR依赖的蛋白质合成，但类似的机制是否在人类神经元发育中发挥作用尚不清楚。这项提议将首先测试来源于人类诱导多能干细胞(HiPSCs)的人视网膜神经节细胞(RGC)是否使用mTOR介导的蛋白质合成来对积极和消极的轴突指导信号做出反应。在目标2中，我们将测试TSC1/TSC2复合体的作用，TSC1/TSC2复合体是mTOR的关键上游负调控因子。为此，我们将通过对TSC患者的成纤维细胞进行重新编程来产生新的HiPSCs系。虽然这项建议将重点放在视网膜节细胞上，但使用这些新的HiPSC株可以研究各种各样的其他细胞和神经元类型。因此，这些重要的新细胞系将成为许多研究人员的宝贵资源，并将通过WiCell提供分布。在目标3中，我们将测试来自TSC hPSC的RGCs是否对目标1中测试的轴突引导线索表现出异常反应。