Monkey Alcohol Tissue Research Resource (MATRR)

 描述（由申请人提供）："猴子酒精组织研究资源"（MATRR）是俄勒冈州健康与科学大学、贝勒大学和维克森林健康科学的多项PI研究资源奖。MATRR的建立是为了向酒精研究界提供从接受相同自愿酒精自我给药方案的猴子队列中产生的组织和数据。在该程序中，猴的平均每日乙醇摄入量（g/kg）分布广泛，包括低、暴饮暴食、重度和非常重度慢性摄入模式。我们的尸检程序是完全完善的，并允许收获大脑切片，快速冷冻或冷冻。 制备用于离体电生理学和神经化学分析。在尸检之前，联系注册用户和其他相关方进行任何定制制备，否则冷冻组织将保留在MATRR中，直到通过专用网络资源提出请求。所有组织都附有有机体和酒精摄入量数据，以帮助快速公布结果。重要的是，MATRR为在神经科学，器官病理学和遗传分析方面具有特定专业知识的实验室提供了一个机会，能够将其研究扩展到具有与人类相似遗传学，发育过程，内分泌学，免疫学，生理学和神经解剖学的灵长类动物模型。因此，MATRR是独特的酒精研究领域，促进酒精诱导的病理机制研究的翻译资源。在组织生成、库存控制、组织请求、组织利用、数据分析、生成的新数据、出版物、新资助项目和新研究方向方面，MATRR资源超出了所有预期。在组织生成方面，目前有来自13个猴群的组织和数据可供索取。在这次更新中，我们建议再增加10个队列，使猴的总数达到248只（182只乙醇，66只对照），以允许遗传和神经成像数据集的临界数量。库存控制得到了独特的基于网络的界面的帮助，该界面还用于管理评估和处理组织请求。我们提供了超过2，100份组织样本，在发送之前提交了MTA，并自动要求及时提交进度报告。该资源在11个研究中心的26名主要研究者的出版物（42篇）和资助申请（33篇）数量方面非常成功。在进一步建立和完善这一资源方面仍有很大的机会。我们的目标是让MATRR生物信息学整合跨学科，器官和个人的数据，以产生慢性酒精使用障碍的已知知识。最后，我们承认，除了为大规模数据分析建立关键数字外，还有机会研究其他组织，如胎儿酒精暴露，以及其他数据集，如静息状态功能成像MRI。

 DESCRIPTION (provided by applicant): The "Monkey Alcohol Tissue Research Resource" (MATRR) is a multiple PI research resource award from Oregon Health & Science University, Baylor University, and Wake Forest Health Science. The MATRR was established to provide the alcohol research community access to tissue and data generated from cohorts of monkeys that have been subjected to the same voluntary alcohol self-administration protocol. In this procedure, monkeys show a wide distribution of average daily ethanol intakes (g/kg) encompassing low, binge, heavy and very heavy chronic intake patterns. Our necropsy procedure is fully refined and allows for the harvesting of brain slices that are rapidly frozen or prepared for ex vivo electrophysiological and neurochemical analyses. Prior to necropsy, registered users and other interested parties are contacted for any custom preparations, otherwise the frozen tissue remain in the MATRR until requests are made through a dedicated web resource. All tissues are accompanied by organismal and alcohol intake data to aid in rapid publication of findings. Importantly, the MATRR provides an opportunity for laboratories with specific expertise in neuroscience, organ pathology, and genetic analyses the ability to extend their research to a primate model with similar genetics, developmental processes, endocrinology, immunology, physiology and neuroanatomy to humans. Thus, the MATRR is unique to the alcohol research field in promoting a translational resource for mechanistic studies of alcohol-induced pathologies. The MATRR resource has exceeded all expectations in terms of tissue generation, inventory control, tissue requests, tissue utilization, data analytics, novel daa generated, publications, newly funded projects and new research directions. In terms of tissue generation, currently there are tissues and data from 13 cohorts of monkeys available for request. In this renewal, we propose to add 10 more cohorts, bringing the total number of monkey to 248 (182 ethanol, 66 control) to allow a critical number for genetic and neuroimaging datasets. The inventory control has been aided by a unique web-based interface that also serves to administratively evaluate and act upon tissue requests. We have provided over 2,100 tissue samples, with MTAs filed prior to sending and timely progress reports automatically requested. The resource has been extremely successful in the number of publications (42) and funded applications (33) from 26 Principal Investigators at 11 sites. There remain great opportunities in further building and refining this resource. Our goal is to have the MATRR bioinformatics integrate data across disciplines, organs and individuals to generate knew knowledge of chronic alcohol use disorders. Finally, we acknowledge that in addition to building critical numbers for large-scale data analyses, the opportunity exists to study additional tissue, such as fetal alcohol exposure, and additional datasets such as resting state functional imaging MRI.