Monkey Alcohol Tissue Research Resource (MATRR)

项目总结 《猴酒组织研究资源》(MATRR)是由 俄勒冈健康与科学大学和贝勒大学。MATRR在#年取得了巨大的成功 为酒精研究社区提供从猴子队列产生的组织和数据的访问权限 已经完成了研究自愿酒精自我给药的标准方案。该过程产生一个 平均每日酒精摄入量(g/kg)的广泛分布和MATRR生物信息学已经建立 可靠、健壮的饮酒类别，包括低、暴饮暴食、重度和非常重度的慢性摄入量 模式。尸检程序是完全完善的，并允许最高质量的所有器官和 体内的组织。在验尸之前，注册用户和其他感兴趣的人被联系以进行定制 组织制剂，否则冷冻和固定的组织将保留在MATRR中，直到通过 专用Web资源。所有组织都附有关于有机和酒精摄入量变量的数据，以帮助 在快速发布调查结果方面。重要的是，MATRR为实验室提供了一个机会，让他们能够 器官病理学、神经科学和基因组学方面的专业知识能够将他们的研究扩展到灵长类动物 遗传、发育过程、内分泌学、免疫学、生理学和 人类的神经解剖学。因此，在酒精研究领域中，MATRR在促进翻译的 酒精引起的病理机制研究的资源。MATRR资源已超过所有 在组织生成、库存控制、组织请求、组织利用、数据分析、 产生的新数据、出版物、新资助的项目和新的研究方向。在这次更新中，我们 建议再增加3个队列，预计将积累20个队列的组织和数据 猕猴(共240只，162只乙醇，78只对照)。这将MATRR定位为填充实验设计 需要大量的个体，例如遗传和活体成像数据集。库存控制 由独特的基于网络的界面辅助，该界面还用于对组织进行管理性评估和操作 请求。我们总共有226个注册用户，从5590个组织中运送了超过3813个组织样本 和数据请求。对于每个接受的请求，都会提交一份MTA，并自动提交及时的进度报告 已请求。在过去的资助中，该资源在出版物数量(48份)方面极为成功 在48个月内)，来自13所独特的大学或研究所的22个不同的实验室。仍然有很棒的 进一步建设和提炼这一资源的机会。我们的目标是让MATRR生物信息学 整合跨学科、跨器官和跨个人的数据，以生成有关慢性饮酒的已知知识 精神错乱。最后，我们承认，除了为大规模数据分析建立关键数字之外， 有机会研究额外的组织(如胎儿)，方案中的变化(如在 禁欲)和其他数据集，如肝脏弹性的超声检查。

PROJECT SUMMARY The “Monkey Alcohol Tissue Research Resource” (MATRR) is a multiple PI research resource award from Oregon Health & Science University and Baylor University. The MATRR has been highly successful in providing the alcohol research community access to tissue and data generated from cohorts of monkeys that have completed a standard protocol to study voluntary alcohol self-administration. The procedure results in a wide distribution of average daily ethanol intakes (g/kg) and the MATRR bioinformatics has established reliable, robust categories of drinking that encompass low, binge, heavy and very heavy chronic intake patterns. The necropsy procedure is fully refined and allows for the highest quality harvesting of all organs and tissues in the body. Prior to necropsy, registered users and other interested parties are contacted for custom tissue preparations, otherwise frozen and fixed tissue remain in the MATRR until requests are made through a dedicated web resource. All tissues are accompanied by data on organismal and alcohol intake variables to aid in rapid publication of findings. Importantly, the MATRR provides an opportunity for laboratories with specific expertise in organ pathology, neuroscience and genomics the ability to extend their research to a primate model with similar genetics, developmental processes, endocrinology, immunology, physiology and neuroanatomy to humans. Thus, the MATRR is unique to the alcohol research field in promoting a translational resource for mechanistic studies of alcohol-induced pathologies. The MATRR resource has exceeded all expectations in terms of tissue generation, inventory control, tissue requests, tissue utilization, data analytics, novel data generated, publications, newly funded projects and new research directions. In this renewal, we propose to add 3 more cohorts, with an expected accumulation of tissues and data from 20 cohorts of monkeys (total n=240 (162 ethanol, 78 control)). This has positioned the MATRR to fill experimental designs requiring large number of individuals, for example genetic and in vivo imaging datasets. The inventory control is aided by a unique web-based interface that also serves to administratively evaluate and act upon tissue requests. In total, we have 226 registered users and have shipped over 3,813 tissue samples from 5,590 tissue and data requests. With each accepted request an MTA is filed and timely progress reports are automatically requested. In the past funding, the resource has been extremely successful in the number of publications (48 in 48 months) from 22 different laboratories at 13 unique universities or Institutes. There remain great opportunities in further building and refining this resource. Our goal is to have the MATRR bioinformatics integrate data across disciplines, organs and individuals to generate knew knowledge of chronic alcohol use disorders. Finally, we acknowledge that in addition to building critical numbers for large scale data analyses, the opportunity exists to study additional tissue (such as fetal), variations in the protocol (such as necropsy in abstinence) and additional datasets such as sonography for liver elasticity.