Tissue Oxygenation and Wound Healing

组织氧合和伤口愈合

• 批准号: 8838821
• 负责人: Chandan K Sen
• 金额: $ 30.03万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2016-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8838821
• 关键词: ACVR1B gene; ARNT gene; Address; Adult; Affect; Cell Hypoxia; Cell Respiration; Cells; Chronic; Clinical; Clinical Research; Code; Collagen; Disease; Endothelial Cells; Enrollment; Epidermis; Etiology; Future; Gene Targeting; Genes; Growth; Healed; Health; Human; Hypoxia; Immigration; Infection; Ischemia; Leg Ulcer; Letters; Longitudinal Studies; Metabolism; MicroRNAs; Mitochondria; Molecular; Mus; Nitric Oxide; Nobel Prize; Ohio; Outcome; Oxidation-Reduction; Oxygen; Pathway interactions; Patients; Peripheral; Reactive Oxygen Species; Research Infrastructure; Resources; Signal Transduction; Skin; Societies; System; Testing; TimeLine; Tissues; Translational Research; United States; Universities; Untranslated RNA; Up-Regulation; Vascular Diseases; Vascular blood supply; Visit; Wound Healing; clinical care; fighting; healing; human tissue; hypoxia inducible factor 1; in vivo; keratinocyte; migration; non-diabetic; novel; prospective; research study; response; tissue oxygenation; transcription factor; validation studies; wound

DESCRIPTION (provided by applicant): ABSTRACT Oxygenation state of the wound tissue is known to influence wound outcomes by a number of mechanisms ranging from enabling oxidative metabolism, fighting infection, hydroxylating collagen, generating nitric oxide, and generating reactive oxygen species which may support redox signaling. The etiology of chronic ischemic wounds is generally multi-factorial of which hypoxia caused by underlying peripheral vasculopathy is a common factor. Hypoxia is generally viewed as being angiogenic. So why does the ischemic wound not close even faster? Although hypoxia may have favorable effects on endothelial cells, the hypoxia-inducible transcription factor HIF specifically induces growth arrest of keratinocytes compromising wound closure. HIF1¿ is abundant in basal keratinocytes at the wound edge and causes growth arrest. Our observation directly implicates hypoxia-inducible microRNA (miRNA) or hypoxamir in arresting keratinocyte function. Specifically, we noted that the hypoxamir miR-210 silences target genes which would arrest growth/migration as well as repress mitochondrial metabolism in keratinocytes. In both mice and chronic wound patients, ischemic wounds showed elevated miR-210 levels. Next, we screened for miR-210 dependent pathways that would impair wound re-epithelialization. We studied the candidate pathways that emerged from murine studies for their relevance to human chronic wounds. Those specific pathways that emerged from murine studies and were successfully validated using human chronic wound-edge tissue were selected for study in this proposal. microRNA (miR or miRNA) represents a class of recently discovered non-coding (nc) genes that are of such extraordinary significance in human health and disease. Whether a set of coding gene would be functional or silenced is decided by specific miRs targeting that set of genes. As we seek to take the study of tissue oxygenation and wound healing to the future, we have chosen to focus on unveiling the significance of hypoxamir miR-210 in regulating wound closure. Three aims are proposed: Aim 1: Characterize mechanisms underlying miR-210 dependent compromised wound re-epithelialization of ischemic wounds. ¿1.1 Ischemic wound-associated elevation of miR-210 silences ISCU1/2 inhibiting mitochondrial metabolism; ¿1.2 Elevated miR-210 silences E2F3 causing p53 activation and inhibition of keratinocyte proliferation; ¿1.3 miR-210 up-regulation silences ACVR1B impeding keratinocyte migration. Aim 2: Determine molecular mechanisms underlying induction of miR-210 by ischemia; Central hypothesis: Wound ischemia induces keratinocyte miR-210 via HIF1¿ and lactate. ¿2.1 HIF-1¿/ARNT and novel co- activators drive hypoxia-induced miR-210 expression; ¿2.2 Lactate drives miR-210 expression. Aim 3: Elevated miR-210 in the epidermis of ischemic human wound edge tissue is associated with poor healing outcome in a setting of standard clinical care. We propose a prospective longitudinal study where adult non-diabetic patients with chronic leg ulcers visiting the OSU Comprehensive Wound Center will be enrolled.

描述（由申请人提供）：摘要已知伤口组织的氧合状态通过多种机制影响伤口结果，所述机制包括氧化代谢、抗感染、羟基化胶原、产生一氧化氮和产生可支持氧化还原信号的活性氧物质。慢性缺血性伤口的病因通常是多因素的，其中由潜在的外周血管病变引起的缺氧是常见因素。缺氧通常被认为是血管生成。那么为什么缺血性伤口不能更快地愈合呢？虽然缺氧可能对内皮细胞有有利的影响，但缺氧诱导型转录因子HIF特异性诱导角质形成细胞的生长停滞，从而影响伤口闭合。HIF 1 <$在伤口边缘的基底角质形成细胞中丰富，并导致生长停滞。我们的观察直接涉及低氧诱导的microRNA（miRNA）或hypoxamir在阻止角质形成细胞的功能。具体而言，我们注意到hypoxamir miR-210沉默了角质形成细胞中会阻止生长/迁移以及抑制线粒体代谢的靶基因。在小鼠和慢性伤口患者中，缺血性伤口显示miR-210水平升高。接下来，我们筛选了会损害伤口上皮再生的miR-210依赖性途径。我们研究了从小鼠研究中出现的候选途径与人类慢性伤口的相关性。在本提案中，选择从小鼠研究中出现并使用人类慢性伤口边缘组织成功验证的那些特定途径进行研究。microRNA（miR或miRNA）代表了一类最近发现的非编码（nc）基因，其在人类健康和疾病中具有非凡的意义。一组编码基因是功能性的还是沉默的，取决于靶向该组基因的特异性miR。当我们寻求将组织氧合和伤口愈合的研究推向未来时，我们选择专注于揭示hypoxamir miR-210在调节伤口闭合中的重要性。提出了三个目的：目的1：表征缺血性伤口的miR-210依赖性受损伤口再上皮化的潜在机制。<$1.1缺血性伤口相关的miR-210沉默ISCU 1/2的升高抑制线粒体代谢;<$1.2升高的miR-210沉默E2 F3导致p53激活和抑制角质形成细胞增殖;<$1.3 miR-210上调沉默ACVR 1B阻碍角质形成细胞迁移。目标二：确定局部缺血诱导miR-210的分子机制;中心假设：伤口局部缺血通过HIF 1 α和乳酸诱导角质形成细胞miR-210。<$2.1 HIF-1 <$/ARNT和新型共激活剂驱动缺氧诱导的miR-210表达;<$2.2乳酸盐驱动miR-210表达。目标3：在标准临床护理环境中，缺血性人类伤口边缘组织的表皮中升高的miR-210与不良愈合结果相关。我们提出了一项前瞻性纵向研究，其中将招募在OSU综合伤口中心就诊的慢性腿部溃疡成人非糖尿病患者。