CELL CYCLE REGULATION OF GOLGI MEMBRANE DYNAMICS
高尔基膜动力学的细胞周期调节
基本信息
- 批准号:9106578
- 负责人:
- 金额:$ 34.34万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-01-01 至 2020-08-31
- 项目状态:已结题
- 来源:
- 关键词:BindingBiochemicalBiological AssayBundlingCell Cycle RegulationCell ProliferationCell SurvivalCell divisionCell physiologyCellsCentrosomeChromatinChromosome SegregationChromosomesDefectDependencyDevelopmentEnsureFundingGenerationsGeneticGenomeGenomic InstabilityGoalsGolgi ApparatusInheritedInterphaseLeadLinkMalignant NeoplasmsMapsMediatingMembraneMembrane ProteinsMetaphaseMicrotubule BundleMicrotubule PolymerizationMicrotubulesMitosisMitoticMitotic CheckpointMitotic spindleMolecularN-terminalNuclearOrganellesPathway interactionsPhenotypePhosphorylationPolymersProcessProphaseProteinsRegulationRoleRunningStructureVesicleWorkalpha Karyopherinsbasecell growthdaughter celldriving forceinsightinterestmutantnext generationnovelnucleocytoplasmic transportpreventpublic health relevancesegregationtraffickingtumorigenesis
项目摘要
DESCRIPTION (provided by applicant): Cell proliferation critically depends on the duplication organelles in interphase and the segregation between the two daughter cells during mitosis. Accurate partitioning of chromosomes and intracellular organelles is crucial to sustain cellular functions over generations. Defects in mitosis can lead to genomic instability and loss of vital organelles, which is commonly associated with the development of cancer. While much progress has been made towards understanding the segregation of chromosomes, the mechanisms that govern the partitioning of vital organelles, in particular of the Golgi, remain largely unknown. The mammalian Golgi is essential for secretion and post-mitotic cell survival depends on the partitioning of a functional Golgi into progeny. Our aim is to define the underlying mechanisms that ensure the faithful partitioning of the single mammalian Golgi during mitosis. At the onset of mitosis, the highly organized Golgi structure vesiculates and reforms after equal partitioning in the two daughter cells. We previously showed that the spindle actively partitions the mammalian Golgi. This process is initiated by the Golgi membrane protein GM130, which locally activates the spindle assembly factor TPX2 to initiate microtubule polymerization. Forming microtubules are further captured and bundled by GM130, thereby linking Golgi membranes to the spindle to ensure the Golgi segregation into the daughter cells. In this proposal we plan to determine the biochemical and mechanistic basis for this process. Our aims are to determine the molecular basis for binding partner and functional switching of GM130 in mitosis; to dissect GM130 functions in microtubule nucleation, bundling and Ran dependency in Golgi-derived spindle assembly; and to elucidate the role of Golgi vesiculation in mitotic progression. Together these studies will provide new molecular mechanistic insights into the regulation of mitosis and the division process of the Golgi apparatus.
描述(由申请方提供):细胞增殖主要依赖于间期细胞器的复制和有丝分裂期间两个子细胞之间的分离。染色体和细胞内细胞器的精确划分对于维持细胞功能的世代至关重要。有丝分裂缺陷可导致基因组不稳定和重要细胞器的丢失,这通常与癌症的发展有关。虽然已经取得了很大的进展,了解染色体的分离,管理重要的细胞器,特别是高尔基体的分区的机制,仍然在很大程度上是未知的。哺乳动物的高尔基体是分泌和有丝分裂后的细胞生存所必需的,取决于功能的高尔基体到后代的分配。我们的目的是确定潜在的机制,确保忠实的分区的单个哺乳动物高尔基体在有丝分裂。在有丝分裂开始时,高度组织化的高尔基体结构囊泡化,并在两个子细胞中平均分配后重新形成。我们以前表明,纺锤体积极分区哺乳动物高尔基体。这一过程是由高尔基体膜蛋白GM 130启动的,它局部激活纺锤体组装因子TPX 2以启动微管聚合。形成的微管被GM 130进一步捕获和捆绑,从而将高尔基体膜连接到纺锤体,以确保高尔基体分离到子细胞中。在这个建议中,我们计划确定这个过程的生物化学和机制基础。我们的目的是确定结合伙伴和功能开关的GM 130在有丝分裂的分子基础;解剖GM 130的微管成核,捆绑和Ran依赖性在高尔基体衍生的纺锤体组装的功能;并阐明高尔基体囊泡化在有丝分裂进程中的作用。这些研究将为有丝分裂和高尔基体分裂过程的调控提供新的分子机制见解。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
JOACHIM SEEMANN其他文献
JOACHIM SEEMANN的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('JOACHIM SEEMANN', 18)}}的其他基金
Cell cycle regulation of Golgi membrane dynamics
高尔基体膜动力学的细胞周期调节
- 批准号:
8026349 - 财政年份:2011
- 资助金额:
$ 34.34万 - 项目类别:
Cell cycle regulation of Golgi membrane dynamics
高尔基体膜动力学的细胞周期调节
- 批准号:
8598095 - 财政年份:2011
- 资助金额:
$ 34.34万 - 项目类别:
Cell cycle regulation of Golgi membrane dynamics
高尔基体膜动力学的细胞周期调节
- 批准号:
8209003 - 财政年份:2011
- 资助金额:
$ 34.34万 - 项目类别:
Cell cycle regulation of Golgi membrane dynamics
高尔基体膜动力学的细胞周期调节
- 批准号:
8401889 - 财政年份:2011
- 资助金额:
$ 34.34万 - 项目类别:
相似海外基金
CAREER: Biochemical and Structural Mechanisms Controlling tRNA-Modifying Metalloenzymes
职业:控制 tRNA 修饰金属酶的生化和结构机制
- 批准号:
2339759 - 财政年份:2024
- 资助金额:
$ 34.34万 - 项目类别:
Continuing Grant
Systematic manipulation of tau protein aggregation: bridging biochemical and pathological properties
tau 蛋白聚集的系统操作:桥接生化和病理特性
- 批准号:
479334 - 财政年份:2023
- 资助金额:
$ 34.34万 - 项目类别:
Operating Grants
Diurnal environmental adaptation via circadian transcriptional control based on a biochemical oscillator
基于生化振荡器的昼夜节律转录控制的昼夜环境适应
- 批准号:
23H02481 - 财政年份:2023
- 资助金额:
$ 34.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Leveraging releasable aryl diazonium ions to probe biochemical systems
利用可释放的芳基重氮离子探测生化系统
- 批准号:
2320160 - 财政年份:2023
- 资助金额:
$ 34.34万 - 项目类别:
Standard Grant
Biochemical Mechanisms for Sustained Humoral Immunity
持续体液免疫的生化机制
- 批准号:
10637251 - 财政年份:2023
- 资助金额:
$ 34.34万 - 项目类别:
Structural and biochemical investigations into the mechanism and evolution of soluble guanylate cyclase regulation
可溶性鸟苷酸环化酶调节机制和进化的结构和生化研究
- 批准号:
10604822 - 财政年份:2023
- 资助金额:
$ 34.34万 - 项目类别:
Enhanced Biochemical Monitoring for Aortic Aneurysm Disease
加强主动脉瘤疾病的生化监测
- 批准号:
10716621 - 财政年份:2023
- 资助金额:
$ 34.34万 - 项目类别:
Converting cytoskeletal forces into biochemical signals
将细胞骨架力转化为生化信号
- 批准号:
10655891 - 财政年份:2023
- 资助金额:
$ 34.34万 - 项目类别:
Chemical strategies to investigate biochemical crosstalk in human chromatin
研究人类染色质生化串扰的化学策略
- 批准号:
10621634 - 财政年份:2023
- 资助金额:
$ 34.34万 - 项目类别:
EAGER: Elastic Electronics for Sensing Gut Luminal and Serosal Biochemical Release
EAGER:用于感测肠腔和浆膜生化释放的弹性电子器件
- 批准号:
2334134 - 财政年份:2023
- 资助金额:
$ 34.34万 - 项目类别:
Standard Grant