High Speed Single Pixel Hyperspectral Spatial Frequency Domain Imaging

 描述(申请人提供)：空间频域成像(SFDI)是一种基于模型的、广域、非接触的方法，用于测量生物组织的吸收、散射和荧光特性。通过测量以不同空间频率和相位投射到样品上的正弦模式的光的衰减(或荧光)，同时确定每个像素的光学属性。在给定波长下的平均询问深度由投影的空间频率控制，并且路径长度的频率依赖差被用来使用计算模型来计算组织光学特性。为提取SFDI图像而处理的图像记录在传统的摄像系统上，该系统成本高昂，数据采集速度有限。SFDI恢复的组织特性随光波长的不同而变化。传统的摄像系统不能分辨光学波长，因此对组织特性的光谱变化的SFDI成像需要切换照明波长并顺序地获取SFDI数据。或者，也可以使用复杂而昂贵的高光谱相机，但对于一般的临床应用来说，成本太高了。为了克服SFDI成像速度的限制并实现高速高光谱SFDI成像，我们将开发用于宽光谱SFDI图像提取的空间频率调制成像(SPIFI)能力。SPIFI利用在空间和时间上调制的光将空间信息编码成调制频率，在照亮物体后，在单像素探测器上收集的光可以被解码形成图像。单像素探测器显示的带宽比相机系统大许多个数量级。大的探测器带宽开启了用单个光电探测器记录空间和光谱信息数据的能力，该探测器可以被处理成高光谱图像。将SPIFI、SFDI和高光谱成像相结合的战略将为定量光谱组织成像提供前所未有的成像速度。此外，应用于照明光的调制格式是自适应的-使单像素高光谱SFDI成为极其灵活的成像平台，可以动态调整成像、速度、分辨率和光谱分辨率，以针对特定目标应用优化成像条件。自适应地优化组织中吸收、散射或荧光的成像并建立深度分辨率的高光谱图像的能力将为组织成像开辟新的能力。

 DESCRIPTION (provided by applicant): Spatial Frequency Domain Imaging (SFDI) is a model-based, wide-field, non-contact method for measuring the absorption, scattering, and fluorescence properties of biological tissue. Optical properties are determined in each pixel simultaneously, by measuring the attenuation (or fluorescence) of sinusoidal patterns of light projected onto the sample at varying spatial frequencies and phases. The mean interrogation depth at a given wavelength is controlled by the spatial frequency of projection, and frequency-dependent differences in path length are used to calculate tissue optical properties using computational models. Images processed to extract SFDI images are recorded on a conventional camera system, which are costly and limited in data acquisition speed. The tissue properties recovered with SFDI vary with optical wavelength. Conventional camera systems are not able to resolve optical wavelengths, so SFDI imaging of the spectral variation in tissue properties would require switching illumination wavelengths and sequentially acquiring SFDI data. Alternatively, complex and expensive hyperspectral cameras can be used, but the cost is prohibitive for general clinical use. To overcome the limitations on SFDI image speed and to enable high speed hyperspectral SFDI imaging, we will develop the capability of SPatIal Frequency modulation for Imaging (SPIFI) to be used for SFDI image extraction with a broad optical spectrum. SPIFI exploits light modulated in space and time to encode spatial information into modulation frequency to that after illuminating an object, light collected on a single pixel detector can be decoded to form images. Single pixel detectors exhibit bandwidths many orders of magnitude larger than camera systems. The large detector bandwidth opens the capability for recording data for spatial and spectral information with a single photodetector that can be processed into hyperspectral images. The strategy of merging SPIFI, SFDI, and hyperspectral imaging will open up capabilities for unprecedented imaging rates of quantitative spectroscopic tissue imaging. In addition, modulation formats applied to the illumination light are adaptable - making single pixel hyperspectral SFDI an extremely agile imaging platform that can adapt the imaging, speed, resolution, and spectral resolution dynamically to optimize imaging conditions for specific target applications. The ability to adaptively optimize imaging of absorption, scattering, or florescence in tissues and build up depth-resolved hyperspectral images will open new capabilities for tissue imaging.