Core D - Protein Production and Interaction

核心 D - 蛋白质生产和相互作用

• 批准号: 9073919
• 负责人: W Sean Davidson
• 金额: $ 11.91万
• 依托单位: VANDERBILT UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-15 至 2021-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9073919
• 关键词: ATP binding cassette transporter 1; ATP-Binding Cassette Transporters; Abbreviations; Apolipoprotein A-I; Apolipoprotein A-II; Apolipoproteins; Arteriosclerosis; Bacteria; Biological; Biotin; CETP gene; Cardiovascular Diseases; Chemicals; Complex; Electrospray Ionization; Enzymes; Eukaryotic Cell; Evaluation; Goals; Green Fluorescent Proteins; High Density Lipoproteins; High Pressure Liquid Chromatography; Human; Individual; Institutes; Knowledge; Laboratories; Lipids; Lipoproteins; Malondialdehyde; Mass Spectrum Analysis; Metabolic Diseases; Mission; Phosphatidylcholine-Sterol O-Acyltransferase; Plasma; Prevention; Production; Program Research Project Grants; Proteins; Public Health; Reagent; Recombinants; Research; Research Personnel; Resolution; Sampling; Science; Services; Site; Structure; Succinimides; Techniques; Universities; abstracting; cost; cost effective; crosslink; design; experience; human disease; improved; innovation; interdisciplinary approach; molecular dynamics; mutant; protein protein interaction; protein structure; reconstitution; technology development; therapeutic development

ABSTRACT: PROTEIN PRODUCTION AND INTERACTION CORE (CORE D) The Protein Production and Interaction Core (Core D) will be led by W. Sean Davidson and will be located in his laboratory at the University of Cincinnati. His lab has extensive and documented experience in all proposed Core services. The Core is designed to provide a consistent and cost-effective set of high quality protein and lipoprotein reagents to PPG investigators in furtherance of the goals of each Project. Core D also provides evaluations of protein:protein interactions by developing and implementing state-of-the-art cross-linking approaches for application across the PPG projects as well as continued technology development with the aim of improving the resolution of structural determinations. The Core will be heavily used by each of the Projects. The goals of Core D are: 1. To express, purify and distribute recombinant apolipoproteins and their mutants produced in bacteria. 2. To express and distribute PON1, CETP, apoA-II and ABCA1 and their mutants produced in eukaryotic cells. 3. To isolate, characterize and distribute lipoproteins and apolipoproteins isolated from human plasma. 4. To generate, characterize and distribute reconstituted HDL lipoproteins. 5. To perform chemical cross-linking analyses on individual proteins, enzymes, transfer proteins and lipoprotein complexes in synergy with the HDL Quantitation Core (Core C). 6. To develop innovative new cross-linking approaches aimed at increasing protein structure resolution.

摘要：蛋白质生产和相互作用核心（核心D） 蛋白质生产和相互作用核心（核心D）将由W。肖恩·戴维森和将位于 他在辛辛那提大学的实验室。他的实验室在所有提议的领域都拥有广泛且有记录的经验 核心服务。核心的目的是提供一个一致的和具有成本效益的一套高品质的蛋白质和 脂蛋白试剂提供给PPG研究者，以促进每个项目的目标。核心D还提供 通过开发和实施最先进的交联来评价蛋白质：蛋白质相互作用 在PPG项目中应用的方法以及持续的技术开发， 提高结构测定的分辨率。每个项目都将大量使用核心。 核心D的目标是： 1.表达、纯化和分配细菌中产生的重组载脂蛋白及其突变体。 2.在真核细胞中表达和分布PON 1、CETP、apoA-II和ABCA 1及其突变体 细胞 3.分离、表征和分配从人血浆中分离的脂蛋白和载脂蛋白。 4.生成、表征和分配重组HDL脂蛋白。 5.对单个蛋白质、酶、转移蛋白和 脂蛋白复合物与HDL定量核心（核心C）协同作用。 6.开发创新的新交联方法，旨在提高蛋白质结构分辨率。