Core D - Protein Production and Interaction

摘要：蛋白质生产与相互作用核心(核心D) 蛋白质生产和相互作用核心(核心D)将由W.肖恩·戴维森领导，将设在 他在辛辛那提大学的实验室。他的实验室在所有建议项目中都有丰富的记录经验 核心服务。核心的设计是为了提供一套一致和具有成本效益的高质量蛋白质和 向PPG研究人员提供脂蛋白试剂，以促进每个项目的目标。核心D还提供 蛋白质的评价：通过开发和实施最先进的交联来实现蛋白质的相互作用 在PPG项目中应用的方法以及持续的技术开发，目的是 提高结构确定的分辨率。核心将被每个项目大量使用。 核心D的目标是： 1.在细菌中表达、纯化和分布重组载脂蛋白及其突变体。 2.在真核细胞中表达和分布PON1、CETP、apoA-II和ABCA1及其突变体 细胞。 3.分离、鉴定和分布人血浆脂蛋白和载脂蛋白。 4.制备、鉴定和分布重组高密度脂蛋白。 5.对单个蛋白质、酶、转移蛋白和 与高密度脂蛋白定量核心(核心C)协同的脂蛋白复合体。 6.开发旨在提高蛋白质结构分辨率的创新的新的交联方法。

ABSTRACT: PROTEIN PRODUCTION AND INTERACTION CORE (CORE D) The Protein Production and Interaction Core (Core D) will be led by W. Sean Davidson and will be located in his laboratory at the University of Cincinnati. His lab has extensive and documented experience in all proposed Core services. The Core is designed to provide a consistent and cost-effective set of high quality protein and lipoprotein reagents to PPG investigators in furtherance of the goals of each Project. Core D also provides evaluations of protein:protein interactions by developing and implementing state-of-the-art cross-linking approaches for application across the PPG projects as well as continued technology development with the aim of improving the resolution of structural determinations. The Core will be heavily used by each of the Projects. The goals of Core D are: 1. To express, purify and distribute recombinant apolipoproteins and their mutants produced in bacteria. 2. To express and distribute PON1, CETP, apoA-II and ABCA1 and their mutants produced in eukaryotic cells. 3. To isolate, characterize and distribute lipoproteins and apolipoproteins isolated from human plasma. 4. To generate, characterize and distribute reconstituted HDL lipoproteins. 5. To perform chemical cross-linking analyses on individual proteins, enzymes, transfer proteins and lipoprotein complexes in synergy with the HDL Quantitation Core (Core C). 6. To develop innovative new cross-linking approaches aimed at increasing protein structure resolution.