Advanced Imaging Core

先进的成像核心

• 批准号: 9550611
• 负责人: Ronald Petralia
• 金额: $ 72.25万
• 依托单位: NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9550611
• 关键词: Affect; Amygdaloid structure; Biological; Brain; Cell Adhesion Molecules; Cells; Collaborations; Core Facility; Data; Development; Diamond; Educational workshop; Electron Microscope; Enzymes; Farming environment; Freeze Substitution; Future; Gap Junctions; Genes; Genetic; Goals; Grant Review; Hair Cells; Heat-Shock Response; Hippocampus (Brain); Image; Institutes; Intramural Research; Japan; Journals; Label; Laboratories; Left; Letters; Light Microscope; Manuscripts; Mitochondria; Molecular; Mutant Strains Mice; National Human Genome Research Institute; National Institute of Child Health and Human Development; National Institute of Neurological Disorders and Stroke; National Institute on Deafness and Other Communication Disorders; Nature; Neurons; Neurosciences; Paper; Performance; Physiology; Potassium Channel; Presynaptic Terminals; Principal Investigator; Proteins; Publications; Publishing; Purines; Recommendation; Reporting; Research; Research Personnel; Research Support; Resources; Retina; Running; SHH gene; Safety; Sampling; Scanning Electron Microscopy; Schizophrenia; Scientist; Services; Specialist; Spectrin; Structure; Supporting Cell; Synapses; Techniques; The Sun; Three-dimensional analysis; Training; Transmission Electron Microscopy; United States National Institutes of Health; Utricle structure; Work; Writing; XLRS1 protein; Yang; Zebrafish; animal facility; base; cannabinoid receptor; exosome; experimental study; immunocytochemistry; inhibitory neuron; interest; medical schools; mutant; neuromast; new technology; programs; ribbon synapse; social anxiety; tissue preparation; tomography; transmission process; uptake

Ronald S. Petralia and Ya-Xian Wang have run the Advanced Imaging Core facility of NIDCD since October 2011. This core is dedicated primarily to collaborating with and training other scientists in NIDCD in studies utilizing transmission electron microscopy (TEM). And we present an annual workshop on TEM as part of the EARssentials program. Some work also is done with other NIH institutes or outside NIH. In addition, Ronald Petralia regularly reviews papers for professional journals and reviews many various documents for NIDCD PIs, reviews grants, writes review papers and letters of recommendation, serves on several NIH committees and helps coordinate lab+office safety for NIDCD (including annual inspections and reports), and performs animal facility inspections as part of his duties in the ACUC. Lisa Cunningham lab: We are looking at effects of heat shock on the release/uptake of exosomes in the utricle. We are doing these studies in collaboration with the postBac IRTA fellow, Nora Welsh, and Andrew Breglio from the lab. Katie Kindt lab: The Core continues periodically to use TEM to study the hair cells and associated supporting cells of the zebrafish neuromasts and utricle. Recently, we published one study on a mutant affecting synaptic ribbon structure in neuromasts. We have another paper involving gap junctions in neuromasts, submitted to Nature Neuroscience. Paul Sieving/ Hongman Song/Ron Bush NIDCD/NEI: Our paper on Retinoschisin was published in Oct. 2016. We continue to perform new TEM studies of the retina associated with various mutant mice. Tom Friedman/Inna Belyantseva lab: We have a collaboration that will be submitted soon; we provided a detailed 3D analysis of stereocilia and rootlets associated with TrioBP mutants; and we performed or assisted with other TEM related to these studies. The Core continues to advise Inna Belyantseva on TEM techniques including tomography, to optimize ultrastructural studies of hair cells and stereocilia. We also assist Inna with tissue preparation, e.g., freeze substitution. Others: Last year, we had worked extensively on studies with Doris Wu, Sharda Yadav, and Kazuya Ono, to look at changes in TEM and SEM structure of stereocilia and kinocilia and the cuticular plate in the utricle of mutant mice. This year, we gave some limited assistance to Kazuya Ono; this may continue in the near future. Also, the Core continued to assist Robstein Chidavaenzi, a fellow in the lab of Bechara Kachar, until he left in June of this year, examining spectrins and associated proteins in stereocilia rootlets. We also provide some assistance to Willy Sun and Evan Krystofiak related to TEM and SEM. And our final study with Matt Kelley and Helen May-Simera was published in Oct. 2016. Other NIH institutes: NIA /Mark Mattson/Pamela Yao lab: We published a paper on effects of sonic hedgehog on mitochondria (with Jennifer Lippincott-Schwartz-now in Janelia Farms). In addition, we published review papers on sonic hedgehog and invaginating presynaptic terminals. We also have a research paper in review on neuronal purine enzymes. We continue to work on various studies of neuronal function. NIHMS Zheng Li/Kaizheng Duan: We recently began a study on mitophagy in the amygdala associated with social anxiety. NICHD Dax Hoffman/Lin Lin: We have done immunogold labeling for localization of DPP6, a potassium channel auxiliary protein with EM immunogold; we will submit a manuscript soon. NINDS Wei Lu/Wenyan Han/Jun Li: We participated in a study on two synaptic cell adhesion molecules associated with GABAergic synapse development in the hippocampus: manuscript in review. NINDS Katherine Roche/Richa Lomash lab: We have been using TEM to study neurolastin function. We are looking at structural changes in the neurons in the brain of the mutant. Others: We have continued to some extent our involvement with studies of Nahid Tayebi/Ellen Sidransky (NHGRI) and Jeff Diamond/Jun Zhang (NINDS). The Core also may work with Gail Seabold (formerly NIDCD/currently OITE), on SALM1 in the retina in collaboration with Florentina Soto (Wash. Univ. School of Medicine). Outside NIH: This Spring, we began a collaboration with Feng Yang and Zhonghua Hu (Lieber Inst. for Brain Devel./Johns Hopkins Med Campus) on genes associated with schizophrenia, examining effects on cultured hippocampal neurons and HEK293 cells. Gavin Rumbaugh/Massimiliano Aceti (Scripps Institute): We submitted a paper on function of Syngap1 for review, based on work that we did with them previously. We train/advise/prepare samples for TEM for Kazuyo Takeda (FDA/CBER/Micr. & Imag. Core), and we expect that this work will lead to collaborations/publications in the future. We also have been planning studies with Jaroslav Blahos (Inst. Exp. Med. ASCR/Czech Rep.) on distribution of a protein associated with synaptic cannabinoid receptors, and with Megan Wyeth (Stanford, CA) on studies of inhibitory neurons in the hippocampus. In addition to collaborations, Ron Petralia serves as a consultant on studies of T. Fujikawa (Japan)-GluRs in hair cell synapses.

自2011年10月以来，Ronald S. Petralia和Ya-xian Wang已运行NIDCD的先进成像核心设施。该核心主要致力于与NIDCD中的其他科学家合作，并在利用传输电子显微镜（TEM）的研究中培训其他科学家。作为Earssentials计划的一部分，我们介绍了TEM的年度研讨会。一些工作也与其他NIH机构或NIH外部进行。此外，罗纳德·佩特里亚（Ronald Petralia）定期审查专业期刊的论文，并审查了许多有关NIDCD PIS的文档，审查赠款，撰写审查论文和推荐信，在几个NIH委员会上任职，并帮助协调NIDCD的LAB+办公室安全（包括年度检查和报告）（包括年度检查和报告），并执行动物设施的检查，并在他的Accuc中执行动物设施的一部分。 丽莎·坎宁安（Lisa Cunningham）实验室：我们正在关注热休克对乌尔特利（Utricle）外泌体释放/摄取的影响。我们正在与实验室的Bacac Irta研究员Nora Welsh和Andrew Breglio合作进行这些研究。 凯蒂·金特（Katie Kindt）实验室：核心继续定期使用TEM研究斑马神经瘤和乌尔特里菌的毛细胞和相关的支撑细胞。最近，我们发表了一项关于影响神经瘤突触色带结构的突变体的研究。我们还有另一篇论文涉及神经神经科学中的差距连接，并提交了自然神经科学。 Paul Sieving/Hongman Song/Ron Bush Nidcd/Nei：我们关于视网膜感染的论文于2016年10月发表。我们继续对与各种突变小鼠相关的视网膜进行新的TEM研究。 汤姆·弗里德曼（Tom Friedman）/inna belyantseva实验室：我们有一项合作，即将提交；我们提供了与三位一体突变体相关的立体胶质和根的详细3D分析。我们对与这些研究相关的其他TEM进行了或协助。核心继续向inna belyantseva提供有关TEM技术在内的TEM技术的建议，以优化毛细胞和立体胶质的超微结构研究。我们还协助INNA进行组织制备，例如冻结取代。 其他：去年，我们与Doris Wu，Sharda Yadav和Kazuya Ono进行了广泛的研究，以研究Stereocilia和Kinocilia的TEM和SEM结构的变化以及突变小鼠的胸膜中的表皮板。今年，我们向Kazuya ono提供了一些有限的帮助。这可能会在不久的将来继续。此外，核心继续协助Bechara Kachar实验室的家伙Robstein Chidavaenzi，直到他于今年6月离开，检查了立体胶根中的谱和相关蛋白质。我们还为Willy Sun和Evan Krystofiak提供了一些帮助。我们与马特·凯利（Matt Kelley）和海伦·梅·塞米拉（Helen May-Simera）的最终研究于2016年10月出版。 其他NIH机构： Nia /Mark Mattson /Pamela Yao实验室：我们发表了一篇关于Sonic Hedgehog对线粒体影响的论文（与Janelia Farms的Jennifer Lippincott-Schwartz-Now一起）。此外，我们发表了有关声波刺猬和突触前终端的评论论文。我们还收到了有关神经嘌呤酶的综述研究论文。我们继续研究神经元功能的各种研究。 Nihms Zheng Li/Kaizheng Duan：我们最近开始研究与社交焦虑相关的杏仁核中的线粒体。 NICHD DAX HOFFMAN/LIN LIN：我们已经进行了免疫金标记，用于DPP6的定位，DPP6是一种具有EM免疫金的钾通道辅助蛋白；我们将尽快提交手稿。 Ninds Wei Lu/Wenyan Han/Jun Li：我们参与了一项研究与Hippocampus中与GABA能突触发展相关的两个突触细胞粘附分子的研究：综述中的手稿。 Ninds Katherine Roche/Richa Lomash Lab：我们一直在使用TEM来研究神经素功能。我们正在研究突变体大脑中神经元的结构变化。 其他：我们在某种程度上继续参与对Nahid Tayebi/Ellen Sidransky（NHGRI）和Jeff Diamond/Jun Zhang（Ninds）的研究。该核心还可以与佛罗伦萨·索托（Florentina Soto）（华盛顿大学医学院）合作，与Retina的Salm1一起使用盖尔·西伯德（Gail Seabold）（以前是NIDCD/目前OETE）。 NIH外面： 今年春天，我们与冯阳和宗教胡（lieber Inst。for Brain Devel./johns Hopkins Med Campus）进行了与精神分裂症相关的基因的合作，检查对培养的海马神经元和HEK293细胞的影响。 Gavin Rumbaugh/Massimiliano Aceti（Scripps Institute）：根据我们之前与他们所做的工作，我们提交了有关Syngap1功能进行审查功能的论文。 我们培训/建议/准备样品为TEM for Kazuyo takeda（FDA/CBER/MICR。＆IMAG。CORE），我们希望这项工作将来会导致合作/出版物。 我们还一直在计划使用Jaroslav Blahos（Inst。Exp。Ascr/Czech Rep。）研究与突触大麻素受体相关的蛋白质的分布，以及Hippocampus中抑制性神经元的研究。除合作外，罗恩·彼得拉里亚（Ron Petralia）还担任毛细细胞突触中的藤川（Japan）glus lurs研究的顾问。