Fully-Integrated, Non-Instrumented Device for Molecular Diagnostics

用于分子诊断的完全集成的非仪器设备

• 批准号: 9055633
• 负责人: Changchun Liu
• 金额: $ 12.84万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-25 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9055633
• 关键词: Address; Adolescent; Adult; Award; Bedside Testings; Biological Assay; Biometry; Blood specimen; Cause of Death; Cellular Phone; Chemistry; Child; Clinical; Clinical Microbiology; Clinical Treatment; Communicable Diseases; Complement; Cytolysis; Data; Dengue; Detection; Developing Countries; Development; Devices; Diagnostic; Disease; Disease Progression; Doctor of Philosophy; Drug resistance; Educational workshop; Electronics; Engineering; Epidemic; Equipment; Fostering; Funding; Generations; Goals; HIV; Healthcare; Heating; Hepatitis C virus; Home environment; Human Resources; Immunoassay; International; K-Series Research Career Programs; Knowledge; Laboratories; Lateral; Lead; Life; Liquid substance; Malaria; Measures; Mechanics; Mediating; Medical Device; Mentors; Microfluidic Microchips; Microfluidics; Molecular; Molecular Biology; Monitor; Nucleic Acids; Patient Monitoring; Patients; Performance; Pharmacotherapy; Plasma; Postdoctoral Fellow; Process; Research; Research Personnel; Research Training; Resources; Sampling; Sensitivity and Specificity; Technology; Test Result; Testing; Time; Training; United States National Institutes of Health; Viral; Viral Load result; Viral load measurement; Virus; Whole Blood; Work; antiretroviral therapy; authority; base; career; chemical reaction; clinical application; clinical practice; communicable disease diagnosis; cost; experience; genetic epidemiology; global health; health care quality; improved; improved outcome; individualized medicine; innovation; instrument; laboratory facility; molecular diagnostics; multidisciplinary; nanofluidic; nanomedicine; nucleic acid detection; pandemic disease; pathogen; point of care; point-of-care diagnostics; programs; resistant strain; screening; skills; skills training; stem; success

DESCRIPTION (provided by applicant): The training and research experience provided by this Mentored Career Development Award (K25) will enable me to successfully transition from a background in chemistry (B.S. and M.S.), electronic engineering (Ph.D.) and mechanical engineering (Postdoc) to become an independent biomedical researcher. To achieve this, I will work closely with my mentors, Dr. Haim H. Bau (an expert in microfluidics and nanofluidics), Dr. Robert Gross (an international authority in infectious disease, with a focus on HIV), Dr. Frederic Bushman (a Molecular Virologist) and Dr. Paul H. Edelstein (a Clinical Microbiology expert). The training includes 1) coursework in genetics, epidemiology, biostatistics, molecular biology and nanomedicine, 2) experimental-skill training in infectious disease assays, molecular biology, and clinical treatment 3) professional development activities through workshops and networking opportunities. The goal of this training is to allow me to gain the knowledge and skills necessary to apply for independent NIH funding (e.g., R01 awards). To complement the training, I propose a research plan aimed at researching and developing a fully-integrated, low-cost, non-instrumented, POC device for HIV viral load measurement using blood samples. This device will be nearly as simple as a 'dipstick' immunoassay with performance that is comparable to that of benchtop PCR. HIV is a significant global health problem. Recent estimates suggest ~33 million people are living with HIV, with 96% residing in the developing world. While antiretroviral therapy is effective and increasingly available, quantitative monitoring of patients is necessary t individualize drug treatment, and to control the emergence and spread of drug-resistant strains of HIV. The level of circulating virus in the blood plasma, or "viral load," is a critical measure used to monitor disease progression and make treatment decisions. Typically, quantitative PCR-based assays, which require expensive equipment, skilled technicians, and laboratory facilities and that are not widely available for point-of-care testing in resource-limited settings are needed to assess viral load. The innovative POC test devices proposed in this K25 will address this need. My career goal is to become an independent, multidisciplinary and well established biomedical researcher with expertise in developing low-cost diagnostic technology for global health care and for individualized medicine.

描述（由申请人提供）：该导师职业发展奖（K25）提供的培训和研究经验将使我能够成功地从化学（学士和硕士）、电子工程（博士）和机械工程（博士后）背景转变为一名独立的生物医学研究员。为了实现这一目标，我将与我的导师 Haim H. Bau 博士（微流控和纳米流控专家）、Robert Gross 博士（传染病领域国际权威，重点研究艾滋病毒）、Frederic Bushman 博士（分子病毒学家）和 Paul H. Edelstein 博士（临床微生物学专家）密切合作。培训包括 1) 遗传学、流行病学、生物统计学、分子生物学和纳米医学课程，2) 传染病检测、分子生物学和临床治疗方面的实验技能培训，3) 通过研讨会和社交机会进行的专业发展活动。这次培训的目的是让我获得申请独立 NIH 资助（例如 R01 奖项）所需的知识和技能。为了补充培训，我提出了一项研究计划，旨在研究和开发一种完全集成、低成本、非仪器化的 POC 设备，用于使用血液样本测量 HIV 病毒载量。该装置几乎与“试纸”免疫测定一样简单，其性能可与台式 PCR 相媲美。艾滋病毒是一个重大的全球健康问题。最近的估计表明，约有 3300 万人感染艾滋病毒，其中 96% 居住在发展中国家。虽然抗逆转录病毒治疗是有效的并且越来越容易获得，但对患者进行定量监测对于个体化药物治疗以及控制艾滋病毒耐药株的出现和传播是必要的。血浆中循环病毒的水平或“病毒载量”是用于监测疾病进展和做出治疗决策的关键指标。通常，需要基于定量 PCR 的检测来评估病毒载量，这种检测需要昂贵的设备、熟练的技术人员和实验室设施，并且在资源有限的环境中无法广泛用于即时检测。 K25 中提出的创新 POC 测试设备将满足这一需求。我的职业目标是成为一名独立的、多学科的、成熟的生物医学研究员，拥有为全球医疗保健和个体化医疗开发低成本诊断技术的专业知识。

项目成果

Nuclemeter: a reaction-diffusion based method for quantifying nucleic acids undergoing enzymatic amplification.

• DOI: 10.1038/srep07335
• 发表时间: 2014-12-05
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Liu C;Sadik MM;Mauk MG;Edelstein PH;Bushman FD;Gross R;Bau HH
• 通讯作者: Bau HH

Molecular Detection of Schistosome Infections with a Disposable Microfluidic Cassette.

• DOI: 10.1371/journal.pntd.0004318
• 发表时间: 2015-12
• 期刊: PLoS neglected tropical diseases
• 影响因子: 3.8
• 作者: Song J;Liu C;Bais S;Mauk MG;Bau HH;Greenberg RM
• 通讯作者: Greenberg RM

Membrane-based, sedimentation-assisted plasma separator for point-of-care applications.

• DOI: 10.1021/ac402459h
• 发表时间: 2013-11-05
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Liu, Changchun;Mauk, Michael;Gross, Robert;Bushman, Frederic D.;Edelstein, Paul H.;Collman, Ronald G.;Bau, Haim H.
• 通讯作者: Bau, Haim H.