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Genetically encoded live cell sensors of chromatin state

染色质状态的基因编码活细胞传感器

基本信息

批准号：
9225678
负责人：
Albert Keung
金额：
$ 17.47万
依托单位：
NORTH CAROLINA STATE UNIVERSITY RALEIGH
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-30 至 2018-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9225678
关键词：
Antibodies Archaeal Proteins Area Binding Binding Proteins Biochemical Biological Assay Biology Biomedical Research Cells Cellular biology Chemicals Chromatin Collection Complex DNA Development Devices Dimensions Disease Event Future Gene Expression Gene Expression Regulation Genes Genome Goals Heterochromatin Histones In Vitro Knowledge Libraries Life Maps Measurement Medicine Modification Molecular Molecular Conformation Monitor Neurons Optics Play Process Property Protein Biochemistry Proteins RNA RNA-Binding Proteins Reading Reagent Regulation Regulator Genes Research Role Signaling Protein System Testing Time Ubiquitin Work Yeasts cell fixing cell type cellular engineering chromatin modification design frontier gene repression inorganic phosphate light microscopy live cell imaging live cell microscopy novel novel strategies novel therapeutic intervention response screening sensor synthetic biology tool yeast two hybrid system

项目摘要

Project Summary Over the past half-century, biologists have generated a critical mass of knowledge and many compelling hypotheses about how chromatin, a collection of proteins and RNA scaffolded to DNA, regulates gene expression. This knowledge has the potential to transform our understanding of development and disease and spur new therapeutic strategies. To realize this potential, new experimental tools are needed to directly test existing hypotheses and reveal the functions of chromatin components. Of particular use would be approaches that directly and specifically track the biochemical and biophysical states of chromatin in live cells. This would unlock our ability to study dynamic changes in chromatin state in response to cellular perturbations. Observing intracellular events in temporal sequence would also enable functional studies demonstrating changes in gene regulation in direct response to changes in chromatin state. Towards this goal, we will develop small proteins that bind specific biochemical chromatin modifications. These binders will be genetically encoded, enabling their use in live cell microscopy. The development of these chromatin binders will support a new frontier in chromatin research. They will build upon the information garnered from static maps of chromatin state and expand our understanding of chromatin into both the temporal and spatial dimensions.

项目总结