Device for rapid extraction of rare pathogens from raw samples

从原始样品中快速提取稀有病原体的装置

• 批准号: 9202652
• 负责人: Jay Kenneth Fisher
• 金额: $ 29.71万
• 依托单位: REDBUD LABS, INC.
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9202652
• 关键词: Affinity; Antibodies; Area; Avidin; Bacteria; Bacterial Drug Resistance; Binding; Binding Sites; Biological Assay; Blood; Blood Volume; Cells; Chemistry; Communicable Diseases; Detection; Devices; Diagnosis; Diagnostic; Electrons; Engineering; Figs - dietary; Geometry; Goals; Gold; Human; Leadership; Letters; Liquid substance; Mannose Binding Lectin; Mannose-Binding Lectins; Measures; Methods; Microfabrication; Modeling; Motion; Nosocomial Infections; Performance; Process; Property; Protocols documentation; Research; Sampling; Scientist; Sepsis; Silicones; Small Business Innovation Research Grant; Surface; System; Techniques; Technology; Time; Whole Blood; Work; antimicrobial; base; clinically relevant; cost; design; improved; improved outcome; magnetic field; molecular diagnostics; novel; novel diagnostics; pathogen; professor; rapid diagnosis

1 of 2 Hospital acquired infection that leads to sepsis is deadly, expensive, and increasingly common. Rapid diagnosis, anti-microbial treatment, and identification of the pathogen strain is key to improving outcomes and reducing costs. Diagnosis and strain identification must improve. Novel techniques for blood culture (BC) and molecular diagnostics (MDx) are promising, but better technology for pathogen purification and concentration from blood will be essential for the next generation of diagnostics. We will develop a device to purify and concentrate pathogens directly from a large volume (≥10 mL) of whole blood. Unlike existing techniques, our technology will achieve the high binding efficiency of a filter with the non- clogging properties of an empty flow cell.

月2日第1 导致败血症的医院获得性感染是致命的，昂贵的，并且越来越多地 共同快速诊断，抗菌治疗和病原体菌株的鉴定是 改善结果和降低成本的关键。诊断和菌株鉴定必须 持续向好血液培养（BC）和分子诊断（MDx）的新技术 有希望，但更好的技术，病原体纯化和浓缩从血液中将是 对下一代诊断至关重要。我们将开发一种设备， 直接从大量（≥10 mL）全血中浓缩病原体。不同于现有 技术，我们的技术将实现过滤器的高结合效率， 空流动池的堵塞特性。