Excitatory and Inhibitory Activity Contributions to Hemodynamic Signals
兴奋性和抑制性活动对血流动力学信号的贡献
基本信息
- 批准号:9534898
- 负责人:
- 金额:$ 4.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-09-01 至 2018-06-30
- 项目状态:已结题
- 来源:
- 关键词:AnimalsAutomobile DrivingBasic ScienceBloodBlood VesselsBlood VolumeBlood flowBrainCalciumCellsCerebrovascular CirculationClinical ResearchCognitiveDataDevelopmentDiseaseElectrophysiology (science)EnsureExcitatory Amino Acid AntagonistsForelimbFoundationsFunctional Magnetic Resonance ImagingFunctional disorderGlutamatesGoalsHealthHumanImageImpairmentKnowledgeLaboratoriesLaser-Doppler FlowmetryMapsMeasurementMeasuresMethodologyMethodsModelingNeuronsPharmaceutical PreparationsPharmacologyPopulationPositioning AttributeProcessPropertyRegulationRoleSensorySignal TransductionSynapsesSynaptic TransmissionTestingWorkblood oxygen level dependentbrain behaviorcerebral blood volumedesignexcitatory neuronexperimental studygamma-Aminobutyric Acidhemodynamicsimaging studyin vivoinhibitory neuroninnovationinterestmouse modelneurovascularnoveloptical imagingoptogeneticsrelating to nervous systemresponsesomatosensorytooltwo-photon
项目摘要
PROJECT SUMMARY/ABSTRACT
Most approaches to assess brain function and connectivity, including functional magnetic resonance imaging
(fMRI) and optical imaging of intrinsic signals (OIS), rely on activity-evoked changes in cerebral blood flow,
blood volume and/or blood oxygenation as indirect measures of neural activity. While these hemodynamic
methods are immensely valuable to map brain function and connectivity in humans and animals, fundamental
gaps remain in our understanding of neuro-vascular mechanisms due to its complexity and methodological
difficulties. This gap in knowledge greatly impedes our understanding of the driving neuronal processes
behind BOLD fMRI findings of brain function, dysfunction and development. The goal of this proposal is to
determine the role of excitatory and inhibitory neuronal activity, including glutamatergic and GABAergic
synaptic activity, in vascular regulation and their representation in hemodynamic signals. To activate specific
neuronal sub-populations in vivo, novel optogenetic mouse models expressing Channelrhodopsin-2 (ChR2) in
cortical excitatory or inhibitory neurons will be used. These optogenetic models will be combined with an
innovative approach to concurrently record neural and vascular signals under pharmacological conditions
designed to modulate glutamatergic and GABAergic synaptic transmission. Neural activity will be measured by
electrophysiology and two-photon calcium imaging. Vascular signals of blood flow will be obtained by laser
Doppler flowmetry, while OIS will be used to image changes in cerebral blood volume and blood oxygenation
(directly analogous to fMRI). These data will then be used to model and integrate excitatory and inhibitory
activity contributions to hemodynamic signals. This work will have a tremendous impact by advancing our
understanding of relevant neuro-vascular mechanisms as well as establish a more concrete framework that
can allow for a deeper understanding of the neuronal processes behind hemodynamic alterations in brain
development and dysfunction.
项目总结/摘要
大多数评估大脑功能和连通性的方法,包括功能性磁共振成像
(fMRI)和内在信号的光学成像(OIS),依赖于脑血流中的活动诱发的变化,
血容量和/或血氧作为神经活动的间接测量。虽然这些血流动力学
方法对于绘制人类和动物的大脑功能和连接非常有价值,
由于神经血管机制的复杂性和方法论上的差异,
困难这种知识上的差距极大地阻碍了我们对驱动神经元过程的理解
脑功能、功能障碍和发育的功能磁共振成像研究结果。本提案的目的是
确定兴奋性和抑制性神经元活动的作用,包括谷氨酸能和GABA能
突触活动,在血管调节和血流动力学信号的代表。激活特定的
在体内神经元亚群中表达视紫红质-2(ChR 2)的新型光遗传学小鼠模型,
将使用皮质兴奋性或抑制性神经元。这些光遗传学模型将与
在药理学条件下同时记录神经和血管信号创新方法
设计用于调节谷氨酸能和GABA能突触传递。神经活动将通过
电生理学和双光子钙成像。利用激光获得血流信号
多普勒血流仪,而OIS将用于成像脑血容量和血氧的变化
(类似于fMRI)。这些数据将被用于建模和整合兴奋性和抑制性
活动对血液动力学信号的贡献。这项工作将产生巨大的影响,
了解相关的神经血管机制,并建立一个更具体的框架,
可以更深入地了解脑血流动力学改变背后的神经过程
发育和功能障碍。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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ALBERTO L VAZQUEZ其他文献
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{{ truncateString('ALBERTO L VAZQUEZ', 18)}}的其他基金
Inhibitory Neuron Sub-populations and Their Influence on Hemodynamic Imaging
抑制性神经元亚群及其对血流动力学成像的影响
- 批准号:
10553650 - 财政年份:2021
- 资助金额:
$ 4.5万 - 项目类别:
Inhibitory Neuron Sub-populations and Their Influence on Hemodynamic Imaging
抑制性神经元亚群及其对血流动力学成像的影响
- 批准号:
10210750 - 财政年份:2021
- 资助金额:
$ 4.5万 - 项目类别:
Inhibitory Neuron Sub-populations and Their Influence on Hemodynamic Imaging
抑制性神经元亚群及其对血流动力学成像的影响
- 批准号:
10372224 - 财政年份:2021
- 资助金额:
$ 4.5万 - 项目类别:
Excitatory and Inhibitory Activity Contributions to Hemodynamic Signals
兴奋性和抑制性活动对血流动力学信号的贡献
- 批准号:
9312330 - 财政年份:2016
- 资助金额:
$ 4.5万 - 项目类别:
Excitatory and Inhibitory Activity Contributions to Hemodynamic Signals
兴奋性和抑制性活动对血流动力学信号的贡献
- 批准号:
9506875 - 财政年份:2016
- 资助金额:
$ 4.5万 - 项目类别:
Functional relationship between neural, metabolic and hemodynamic responses
神经、代谢和血流动力学反应之间的功能关系
- 批准号:
8094405 - 财政年份:2010
- 资助金额:
$ 4.5万 - 项目类别:
Functional relationship between neural, metabolic and hemodynamic responses
神经、代谢和血流动力学反应之间的功能关系
- 批准号:
8467764 - 财政年份:2010
- 资助金额:
$ 4.5万 - 项目类别:
Functional relationship between neural, metabolic and hemodynamic responses
神经、代谢和血流动力学反应之间的功能关系
- 批准号:
8261713 - 财政年份:2010
- 资助金额:
$ 4.5万 - 项目类别:
Functional relationship between neural, metabolic and hemodynamic responses
神经、代谢和血流动力学反应之间的功能关系
- 批准号:
7989208 - 财政年份:2010
- 资助金额:
$ 4.5万 - 项目类别:
Functional relationship between neural, metabolic and hemodynamic responses
神经、代谢和血流动力学反应之间的功能关系
- 批准号:
8662324 - 财政年份:2010
- 资助金额:
$ 4.5万 - 项目类别:
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