Host-derived biomarker signatures to differentiate acute viral, bacterial, and fungal infection

工作说明书 目前迫切需要改进的诊断试验，以评估发热的病因。 特别是在免疫功能低下的宿主中。该项目将开发基于主机的 生物标记物签名能够识别感染的存在和病原体类别， 免疫功能低下的脆弱人类受试者，从而可能允许更早， 定向（因此更有效）治疗以及减少潜在有害的需求 经验性抗感染治疗。在这项研究中，我们将a）进行高维基因表达 分析已证实存在念珠菌血症、病毒感染、细菌感染的高危宿主的现有样本 感染或非感染性疾病，B）发展基于外周血基因表达 以及c）生成能够 区分四种临床状态：病毒感染、细菌感染、真菌感染（念珠菌血症） 非传染性疾病。所附的工作提供了一个真正的范式转变的方式发烧 这些高危人群的疾病得到诊断和管理。

Statement of Work There is an urgent need for improved diagnostic tests for the evaluation of the etiology of febrile states, particularly in immunocompromised hosts. This project will develop host-based biomarker signatures capable of identifying the presence and pathogen class of infections in vulnerable immunocompromised human subjects, thus potentially allowing for earlier, more directed (and thus more effective) therapy as well as reducing the need for potentially harmful empiric anti-infective therapy. In this study we will a) perform high dimensional gene expression analysis on existing samples of at-risk hosts with proven candidemia, viral infection, bacterial infection, or noninfectious illness, b) develop peripheral blood-based gene expression signatures of candidemia and c) generate a combined multinomial classifier capable of differentiating four clinical states: viral infection, bacterial infection, fungal infection (candidemia) and noninfectious illness. The enclosed work offers a true paradigm shift in the way febrile illnesses in this high-risk population are diagnosed and managed.