Integrated photoacoustic and fluorescence imaging system for anatomical, functional, and molecular characterization of murine models
Integrated photoacoustic and fluorescence imaging system for anatomical, functional, and molecular characterization of murine models
批准号:
9201342
负责人:
Sergey A Ermilov
金额:
$21.15万
依托单位国家:
美国
项目类别:
财政年份:
2017
资助国家:
美国
项目状态:
已结题
起止时间:
2017-09-15 至 2019-09-16
关键词:
AddressAnatomyAnimal ExperimentationAnimal ModelAreaBiologicalBiologyBioluminescenceBiomedical ResearchBlood VesselsBreast Cancer ModelCarbon nanoparticleCardiovascular systemCase StudyCodeDataDetectionDevelopmentDevelopmental BiologyDevicesDimensionsEngineeringFluorescenceFunctional ImagingGenerationsGoldHemoglobinImageImageryImaging DeviceImaging TechniquesImaging technologyIndividualInferiorKidneyLabelLightLipidsLongitudinal StudiesMalignant NeoplasmsMapsMeasurementMedicineMelaninsMetastatic breast cancerMethodologyModalityModelingMolecularMusNeoplasm MetastasisOptical MethodsOrganOutcomePeripheralPhasePhotosensitivityPopulation ResearchProceduresProcessQuantum DotsReporter GenesResearchResearch PersonnelResolutionRodent ModelScanningSentinel Lymph NodeSkinSmall Business Innovation Research GrantSpleenStructureSystemTechniquesTechnologyTestingThree-Dimensional ImageThree-Dimensional ImagingTissue EngineeringToxicologyTreesUltrasonographyValidationWateranatomic imaginganimal model developmentbasebioimagingcancer cellchromophoreclinical developmentcommercializationcostdata acquisitiondesignexperiencefluorescence imagingfluorophoregraphical user interfacehigh resolution imaginghuman diseaseimaging approachimaging modalityimaging systemin vivoin vivo imagingin vivo optical imaginginnovationinstrumentinstrumentationmolecular imagingmouse modelnanoparticlenoveloptical imagingoptoacoustic tomographyphotoacoustic imagingplasmonicspre-clinicalpre-clinical researchpreclinical studyprototypereconstructionresearch and developmenttissue regenerationtwo-dimensionalwhole body imaging
中文摘要
总结
PhotoSound Technologies公司建议开发一种新型成像模式来进行表征和预处理
小鼠模型的临床研究。该技术将能够实现三维功能,
荧光标记和报告基因的分子成像,在强大的解剖结构上以高保真度映射
组织,例如皮肤、中央和外周脉管系统和内部器官。开发的仪器
可用于广泛的临床前研究,包括癌症,毒理学,组织工程和
再生、心血管和发育生物学。
光学体内成像方法(荧光和生物发光)在生物医学领域非常受欢迎。
研究人员将其作为经济实惠、方便且非常灵敏的分子成像工具用于临床前研究,
建立动物模型。然而,它们的独立应用受到空间分辨率差的阻碍
以及图像的二维性所施加的限制。一种高分辨率活体3D成像方法,
它可以很容易地与光学成像集成在一个单一的仪器,将有很大的影响,
整个小动物研究领域。
光声层析成像是一种新兴的生物医学成像模式,具有以下所有要求:(1)
3D全身图像的分辨率为150-500 µm;(2)能够使用相同的仪器激发
荧光和光声效应的产生;(3)在不到1分钟内进行3D扫描。然而,在体内
与常规荧光技术相比,检测荧光团的灵敏度较差。我们的建议
是基于开创性的共同注册的整合荧光和光声模态在一个单一的
紧凑的3D配置(PAFT-3D)克服了每种技术的缺点。
第一阶段项目分为三个具体目标,评估以下方面的可行性:(1)技术
用于在单个紧凑型中共同配准3D光声层析成像和荧光的实现
(2)对小鼠的荧光团和解剖结构进行成像,
分辨率超过已建立的荧光成像的分辨率;(3)提供有价值的成像信息,
临床前动物模型的开发和表征,病例研究重点是小鼠模型,
转移性乳腺癌
第二阶段将集中于开发和体内验证一种具有灵敏度和
针对动物模型开发和临床前研究的各个领域进行了优化的成像程序。
最终的商业系统将能够在体内可视化和分析天然血红蛋白,荧光团,
纳米颗粒和其他用于跟踪、绘图和纵向研究的光敏结构。的
PAFT-3D的价值主张将不仅建立在独特性和技术优势的基础上,
产品,但也通过使其负担得起的广泛人口的研究小组和中心。
英文摘要
SUMMARY
PhotoSound Technologies, Inc. proposes to develop a novel imaging modality for characterization and pre-
clinical research of murine models. The technology will be capable of three-dimensional functional and
molecular imaging of fluorescent labels and reporter genes mapped with high fidelity over robust anatomical
structures, such as skin, central and peripheral vasculature, and internal organs. The developed instrument
could be used in broad spectrum of pre-clinical research including cancer, toxicology, tissue engineering and
regeneration, cardiovascular and developmental biology.
Optical in vivo imaging methods (fluorescence and bioluminescence) found great popularity among
researchers as affordable, convenient, and very sensitive molecular imaging tools for pre-clinical studies and
development of animal models. However, their stand-alone application is impeded by poor spatial resolution
and limitations imposed by two-dimensionality of the images. A high-resolution in vivo 3D imaging method,
which could be easily integrated with optical imaging in a single instrument, would have a great impact on the
entire field of small animal research.
Photoacoustic tomography is an emerging biomedical imaging modality that has all those requirements: (1)
150-500 µm resolution of 3D whole body images; (2) Ability to use the same instrumentation for excitation of
fluorescence and generation of photoacoustic effect; (3) 3D scans in less than 1 minute. However, its in vivo
sensitivity to detection of fluorophores is inferior as compared to regular fluorescence techniques. Our proposal
is based on pioneering co-registered integration of fluorescence and photoacoustic modalities in a single
compact 3D configuration (PAFT-3D) defeating shortcomings of each individual technology.
The Phase I project is organized in three specific aims assessing feasibility of: (1) Technological
implementation for co-registered 3D photoacoustic tomography and fluorescence in a single compact
instrument for imaging live anesthetized mice; (2) Imaging fluorophores and anatomical structures of mice with
resolution exceeding that of established fluorescence imaging; (3) Providing imaging information valuable for
development and characterization of pre-clinical animal models with case study focused on murine models of
metastatic breast cancer.
Phase II will be focused on development and in vivo validation of a commercial instrument with sensitivity and
imaging procedures optimized for various areas of animal model development and preclinical research.
Ultimate commercial system will enable in vivo visualization and analysis of native hemoglobin, fluorophores,
nanoparticles, and other photosensitive constructs used for tracking, mapping, and longitudinal studies. The
value proposition of the PAFT-3D will be built not only around uniqueness and technological superiority of the
product, but also by making it affordable for a broad population of research groups and centers.
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会议论文
Integrated photoacoustic and fluorescence imaging system for anatomical, functional, and molecular characterization of murine models
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批准号:10680593
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项目类别:
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资助金额:$92.68万
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财政年份:2017
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负责人:Sergey A Ermilov
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依托单位:
海外基金