Phosphoproteomics Core

摘要 本计划项目组的总体目标是了解潜在的机制 肾液、电解质和大分子转运。激酶和磷酸酶是必不可少的 这一过程的调节者，因此，延伸到它们的目标磷酸化位点是 对于机械论的洞察很重要。此核心将通过为项目组带来新的 在确定和表征磷酸化位点方面的能力和专业知识 蛋白质和蛋白质组。杰西·莱因哈特博士将担任磷酸蛋白质组学 核心总监，将带来磷酸化图谱、定量蛋白质组学方面的专业知识，以及 计划项目组的磷蛋白合成。莱因哈特博士最近开发了一种新的 能够将磷酸丝氨酸特定地结合到蛋白质中的技术。这 技术使在大肠杆菌菌株中合成生理相关的磷蛋白成为可能 具有扩展的遗传密码。这项独特的技术和最近发布的下一项- 该计划项目组将独家提供生成磷化丝氨酸技术。 成熟的磷蛋白组学技术与新的磷蛋白的结合 合成技术将实现一种独特的方法来验证和进一步探索 蛋白质磷酸化的机制。核心将是良好的学习环境和 让来自计划项目组的受训人员与 莱因哈特实验室。这一培训环境将提供接触最先进技术的途径 项目组的个别成员。莱因哈特博士的研究兴趣密切一致 这个项目组的目标是，除了技术之外，还有独特的实验室环境 是否为计划项目组增加了价值。

Abstract The overall goal of this Program Project Group is to understand the mechanisms underlying renal fluid, electrolyte and macromolecule transport. Kinase and phosphatases are essential regulators of this process and thus, by extension, their target phosphorylation sites are important for mechanistic insight. This core will add value to the project group by bringing new capabilities and expertise in the identification and characterization of phosphorylation sites in proteins and proteomes. Dr. Jesse Rinehart will assume the role as the Phosphoproteomics Core Director and will bring expertise in phosphorylation mapping, quantitative proteomics, and phosphoprotein synthesis to the Program Project Group. Dr. Rinehart recently developed a new technology which enables site-specific incorporation of phosphoserine into proteins. This technology enables the synthesis of physiologically relevant phosphoproteins in an E. coli strain with an expanded genetic code. This unique technology and a recently published next- generation phosphoserine technology will be uniquely available to this Program Project Group. The combinations of established phosphoproteomics technologies and novel phosphoprotein synthesis technologies will enable a unique approach to validation and further exploration of the mechanisms of protein phosphorylation. The core will be an excellent learning environment and bring trainees from the Program Project Groups in close contact with proteomics experts in the Rinehart lab. This training environment will provide access to state-of-the-art technology to the individual members of the Project Group. Dr. Rinehart's research interests are closely aligned with the aims of this project group and, in addition to the technology, the unique lab environment is value added to the Program Project Group.