Phosphoproteomics Core

磷酸化蛋白质组学核心

• 批准号: 9319033
• 负责人: Jesse Rinehart
• 金额: $ 15.63万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9319033
• 关键词: Blood Pressure; Cell Cycle; Cells; Collaborations; Detection; Diabetes Mellitus; Electrolytes; Emerging Technologies; Environment; Equipment; Escherichia coli; Experimental Designs; Genetic Code; Goals; Health; Homeostasis; Human; Hypertension; Individual; Ion Transport; Kidney; Knowledge; Liquid substance; Malignant Neoplasms; Mediating; Methodology; Methods; Molecular; Phosphoproteins; Phosphoric Monoester Hydrolases; Phosphorylation; Phosphorylation Site; Phosphoserine; Phosphotransferases; Physiological; Preparation; Process; Proteins; Proteome; Proteomics; Protocols documentation; Publishing; Renal function; Research; Research Personnel; Role; Sampling; Services; Signal Transduction; Site; Source; Stimulus; System; Technology; Training; Validation; Work; educational atmosphere; experience; information processing; insight; interest; macromolecule; member; neuronal excitability; new technology; next generation; novel; phosphoproteomics; programs

Abstract The overall goal of this Program Project Group is to understand the mechanisms underlying renal fluid, electrolyte and macromolecule transport. Kinase and phosphatases are essential regulators of this process and thus, by extension, their target phosphorylation sites are important for mechanistic insight. This core will add value to the project group by bringing new capabilities and expertise in the identification and characterization of phosphorylation sites in proteins and proteomes. Dr. Jesse Rinehart will assume the role as the Phosphoproteomics Core Director and will bring expertise in phosphorylation mapping, quantitative proteomics, and phosphoprotein synthesis to the Program Project Group. Dr. Rinehart recently developed a new technology which enables site-specific incorporation of phosphoserine into proteins. This technology enables the synthesis of physiologically relevant phosphoproteins in an E. coli strain with an expanded genetic code. This unique technology and a recently published next- generation phosphoserine technology will be uniquely available to this Program Project Group. The combinations of established phosphoproteomics technologies and novel phosphoprotein synthesis technologies will enable a unique approach to validation and further exploration of the mechanisms of protein phosphorylation. The core will be an excellent learning environment and bring trainees from the Program Project Groups in close contact with proteomics experts in the Rinehart lab. This training environment will provide access to state-of-the-art technology to the individual members of the Project Group. Dr. Rinehart's research interests are closely aligned with the aims of this project group and, in addition to the technology, the unique lab environment is value added to the Program Project Group.

摘要 本计划项目组的总体目标是了解 肾液、电解质和大分子转运。激酶和磷酸酶是必需的 因此，通过延伸，它们的靶磷酸化位点是 对机械洞察力很重要。这个核心将通过带来新的 磷酸化位点的鉴定和表征的能力和专业知识， 蛋白质和蛋白质组。杰西·莱因哈特博士将担任磷酸蛋白组学 核心总监，并将带来磷酸化图谱，定量蛋白质组学， 磷蛋白合成项目组。莱因哈特博士最近开发了一种新的 该技术能够将磷酸丝氨酸位点特异性地掺入到蛋白质中。这 该技术使得能够在大肠杆菌中合成生理相关的磷蛋白。杆菌菌株 拥有扩展的遗传密码这项独特的技术和最近发表的下一个- 第二代磷酸丝氨酸技术将是该计划项目组唯一可用的技术。 已建立的磷蛋白质组学技术和新的磷蛋白的组合 合成技术将使一种独特的方法来验证和进一步探索的 蛋白质磷酸化的机制。核心将是一个良好的学习环境， 使来自计划项目组的学员与蛋白质组学专家密切接触， 莱因哈特实验室。这一培训环境将为培训人员提供最先进的技术， 项目组的个别成员。莱因哈特博士的研究兴趣 这个项目组的目标，除了技术，独特的实验室环境， 为项目组增值。