Fidelity of Repair within Repetitive DNA

这项建议的重点是几个突变的变化伴随着DNA修复芽殖酵母。DNA 修复是由半乳糖调节的位点特异性HO内切酶的表达诱导的，产生一个单一的 双链断裂（DSB）。一个主要的目标是了解与模板相关的复杂突变 在基因转换过程中发生的转换。将研究两种类型的修复：1。拟回文 基因转换过程中的突变形成和2.染色体间微同源介导模板 在基因转换过程中的转换。将筛选解旋酶和其他修复因子的遗传分析， 找到控制这两个事件水平的蛋白质，沿着探索染色质的作用。一 第二个目标是了解基因转换过程中重复拷贝数的变化，这是由我们的研究动机所激发的。 最近发现DSB断裂修复和缺口修复之间的重要差异。协同 Mitch McVey，这个项目的另一个成员，他专注于果蝇的DSB修复，我们将 评估导致重复序列之间缺失的失败缺口修复的频率， 复制区域

This proposal focuses on several mutational changes accompanying DNA repair in budding yeast. DNA repair is induced by galactose-regulated expression of the site-specific HO endonuclease, creating a single double-strand break (DSB). One major goal is to understand complex mutations associated by template switching that occur during gene conversion. Two types of repair will be studied: 1. Quasipalindrome mutation formation during gene conversion and 2. Interchromosomal microhomology-mediated template switching during gene conversion. Genetic analysis of helicases and other repair factors will be screened to find proteins that control the level of these two events, along with an exploration of the role of chromatin. A second goal is to understand changes in repeat copy number during gene conversion, motivated by our recent discovery of important differences between DSB break repair and gap repair. In collaboration with Mitch McVey, another member of this Program Project who focuses on DSB repair in fruit flies, we will assess the frequency of abortive gap repair leading to deletions between repeated sequences within the copied region.