Exploring the diversity of iridoid compounds in blueberry for human health benefits

探索蓝莓中环烯醚萜化合物的多样性对人类健康的益处

• 批准号: 9319210
• 负责人: Courtney Price Leisner
• 金额: $ 6.37万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2018-08-03
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9319210
• 关键词: Anabolism; Anti-Bacterial Agents; Anti-Inflammatory Agents; Anti-inflammatory; Antioxidants; Berry; Biochemical; Bioinformatics; Biological; Blueberries; Chronic Disease; Clinical Research; Cranberries; Economics; Enzymes; Ericaceae; Family; Food; Fruit; Future; Genes; Genetic; Genomics; Health Benefit; Healthcare; Heart Diseases; Human; Improve Access; Inflammation; Lingonberry; Link; Metabolism; Molecular Genetics; Natural Products; North America; Pathway interactions; Pharmaceutical Cares; Pharmacology; Plants; Prize; Production; Reporting; Research; Resveratrol; Surveys; United States; Vaccinium; Vitis; Work; cancer risk; improved; insight; member; neglect; public health relevance; transcriptomics

 DESCRIPTION (provided by applicant): Blueberry (Vaccinium corymbosum) is an economically important fruit crop that is native to North America. Other members of the Vaccinium genus, including cranberry (V. macrocarpon) and lingonberry (V. vitis-idaea), also have significant economic importance due to the use of their fruits in food production, pharmaceuticals and health care products. Fresh market production of blueberries in the United States was valued at $781 million in 2011 and was planted over 77,000 acres, and has significantly grown in the last 20 years. In addition to its commercial value, blueberries are prized for their positive health benefits. Blueberries contain high levels of antioxidants, which can reduce inflammation caused by chronic diseases such as the antioxidant resveratrol, which has been linked to a decreased risk of cancer and heart disease. Iridoids are another class of known pharmacologically important compounds that have recently been found in blueberries and cranberries. Iridoids are present in over 15 plant families and are potent natural products with a wide range of biological activities in humans including, anticancer, antibacterial and anti-inflammatory. Despite their known human health benefits, iridoids are a neglected component of blueberry research. Therefore, the broad, long-term objective of the proposed work is to improve the biochemical, molecular, and genetic understanding of iridoid production in blueberry to enable improved access to these compounds for future clinical research. To reach these objectives, this proposal has three specific aims: 1) survey iridoid compounds in a diversity panel of Vaccinium spp. using biochemical analyses to characterize the range of iridoid compounds, 2) identify the genes underlying iridoid biosynthesis in blueberry through genomic, transcriptomic and bioinformatics analyses, and 3) perform functional characterization of the tailoring enzymes involved in the specific iridoid biosynthetic pathway in blueberry. Overall, this project will provide new insights into iridoid production in blueberries and related Vaccinium species, and provide an understanding of the genetic basis for iridoid metabolism in an economically important fruit crop.

 描述（由申请人提供）：蓝莓（Vaccinium corymbosum）是一种经济上重要的水果作物，原产于北美。越橘属的其他成员，包括蔓越莓 (V. macrocarpon) 和越橘 (V. vitis-idaea)，由于其果实可用于食品生产、药品和保健品，因此也具有重要的经济重要性。 2011 年，美国蓝莓的新鲜市场产量价值 7.81 亿美元，种植面积超过 77,000 英亩，在过去 20 年中显着增长。除了其商业价值外，蓝莓还因其积极的健康益处而受到重视。蓝莓含有高水平的抗氧化剂，可以减少慢性疾病引起的炎症，例如抗氧化剂白藜芦醇，它与降低癌症和心脏病的风险有关。环烯醚萜类化合物是另一类已知的具有重要药理作用的化合物，最近在蓝莓和蔓越莓中发现。环烯醚萜存在于超过 15 个植物科中，是有效的天然产物，对人类具有广泛的生物活性，包括抗癌、抗菌和抗炎。尽管环烯醚萜类化合物已知对人类健康有益，但它却是蓝莓研究中被忽视的一个组成部分。因此，拟议工作的广泛、长期目标是提高对蓝莓环烯醚萜生产的生化、分子和遗传学认识，以便更好地获取这些化合物用于未来的临床研究。为了实现这些目标，该提案具有三个具体目标：1）调查越橘属多样性组中的环烯醚萜化合物。使用生化分析来表征环烯醚萜化合物的范围，2) 通过基因组、转录组和生物信息学分析识别蓝莓中环烯醚萜生物合成的基因，以及 3) 对参与蓝莓中特定环烯醚萜生物合成途径的剪裁酶进行功能表征。总体而言，这 该项目将为蓝莓和相关越橘品种的环烯醚萜生产提供新的见解，并提供对经济上重要的水果作物中环烯醚萜代谢的遗传基础的理解。