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Characterizing the ion-pair dynamics and their roles in protein-DNA association

表征离子对动力学及其在蛋白质-DNA 关联中的作用

基本信息

批准号：
9253410
负责人：
Junji Iwahara
金额：
$ 24万
依托单位：
UNIVERSITY OF TEXAS MED BR GALVESTON
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-04-01 至 2018-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9253410
关键词：
Address Affinity Behavior Biological Biological Models Biological Process Biophysics Catalysis Cations Chemicals Chemistry Collaborations Color Complex Computer Simulation DNA DNA Binding DNA Binding Domain DNA Structure DNA-Protein Interaction Drosophila genus Drug Design Entropy Evolution Fluorescence Future Goals Guanine Human Human Engineering Hydrogen Bonding Ions Knowledge Lead Life Macromolecular Complexes Methods Molecular Molecular Conformation Motion Nucleic Acids Oxygen Pharmaceutical Preparations Play Preclinical Drug Evaluation Process Protein Engineering Proteins Protons RNA Research Research Project Grants Role Side Site Sodium Chloride Solvents Sulfur System TFF1 gene Testing Therapeutic Validation Zinc Fingers atomic interactions base biophysical techniques design driving force engineering design experimental study homeodomain human data improved inorganic phosphate ion dynamics macromolecule molecular dynamics molecular recognition phosphorodithioic acid protein function protein structure systems research three dimensional structure transcription factor

项目摘要

DESCRIPTION (provided by applicant): Ion pairing is one of the most fundamental atomic interactions for biological macromolecules to execute their functions. Numerous three-dimensional structures of macromolecular complexes show the presence of ion pairs (also known as salt bridges) at functionally important sites, suggesting that ion pairs play significant roles in molecular association, recognition and catalysis. Crucial intermolecular ion pairs are also found in many protein-drug complexes. Thus, deeper knowledge of ion pairs can enable more successful macromolecular engineering and drug design for future human therapeutics. Toward this end, the current project brings together three research groups with complementary expertise to understand ion-pair dynamics at protein-DNA interfaces and their roles in protein-DNA association. Formation of ion pairs between protein and DNA along with the release of counterions is the major driving force for many protein-DNA association processes. The PI's group recently developed NMR methods for characterizing side-chain dynamics involving hydrogen bonds and ion pairs. The research in this project is designed to test our central hypothesis that the ion-pair dynamics is entropically important for protein-DNA association. Using NMR and other solution-biophysical methods together with computation and nucleic acid chemistry, the research team will study the dynamics of natural and unnatural ion pairs at molecular interfaces and their impact on protein-DNA association. The specific aims in this project are 1) to characterize the dynamics of ion pairs between protein and DNA; 2) to delineate motional changes of ionized groups in molecular recognition of DNA; and 3) to elucidate the mechanism by which oxygen-to-sulfur substitution in DNA phosphate enhances protein-DNA affinity. Using the DNA-binding domains of Egr-1, HoxD9, and Antp proteins as model systems, the research team will study the ion-pair dynamics and their roles in protein-DNA association for two major classes of eukaryotic transcription factors: zinc-finger (Egr-1) and homeodomain (HoxD9 and Antp) proteins. Comparison of data for human HoxD9 and fruit fly Antp homeodomains will also allow us to examine to what extent ion pair dynamics are conserved though evolution. The research team will also validate molecular dynamics force-field parameter sets by comparing the experimental and computational results on the ion-pair dynamics. This project will substantially advance knowledge of ion pairs in biological macromolecular systems. The new knowledge will facilitate engineering of proteins and nucleic acids for human therapeutics. Experiment-based validation of the force-field parameters relevant to ion pairs can lead to improvement of in silico screening of drugs involving ion pairs. Thus, a broad range of biomedical fields will benefit from this project.

描述(申请人提供)：离子配对是生物大分子执行其功能的最基本的原子相互作用之一。许多大分子络合物的三维结构表明，离子对(也称为盐桥)存在于功能重要的位置，这表明离子对在分子缔合、识别和催化中发挥着重要作用。在许多蛋白质-药物复合体中也发现了关键的分子间离子对。因此，对离子对更深入的了解可以为未来的人类治疗提供更成功的大分子工程和药物设计。为此，目前的项目汇集了三个具有互补专业知识的研究小组，以了解蛋白质-DNA界面上的离子对动力学及其在蛋白质-DNA结合中的作用。蛋白质和DNA之间离子对的形成以及反离子的释放是许多蛋白质-DNA结合过程的主要驱动力。Pi‘s小组最近开发了核磁共振方法来表征涉及氢键和离子对的侧链动力学。这个项目的研究旨在检验我们的中心假设，即离子对动力学对蛋白质-DNA结合具有极大的重要性。研究小组将利用核磁共振和其他溶液生物物理方法，结合计算和核酸化学，研究分子界面上自然和非自然离子对的动力学及其对蛋白质-DNA结合的影响。本项目的具体目标是1)表征蛋白质与DNA之间的离子对动力学；2)描述DNA分子识别中电离基团的运动变化；3)阐明DNA磷酸中氧硫取代增强蛋白质-DNA亲和力的机制。研究小组将以Egr-1、HoxD9和ANTP蛋白的DNA结合域为模型系统，研究两类主要真核转录因子：锌指蛋白(Egr-1)和同源结构域蛋白(HoxD9和ANTP)的离子对动力学及其在蛋白质-DNA结合中的作用。对人类HoxD9和果蝇ANTP同源结构域的数据比较也将使我们能够检查离子对动力学在进化过程中保守到什么程度。研究小组还将通过比较离子对动力学的实验和计算结果来验证分子动力学力场参数集。该项目将极大地提高对生物大分子系统中离子对的认识。这一新知识将促进用于人类治疗的蛋白质和核酸工程。基于实验的与离子对相关的力场参数的验证可以改善涉及离子对的药物的电子筛选。因此，广泛的生物医学领域将从该项目中受益。